Expression and characterization of the key enzymes involved in 2-benzoxazolinone degradation by Pigmentiphaga sp. DL-8

Dong, Weiliang; Liu, Kuan; Liu, Jiawei; Shi, Zhoukun; Xin, Fengxue; Zhang, Wenming; Ma, Jiangfeng; Wu, Hao; Wang, Fei; Jiang, Min

doi:10.1016/j.biortech.2017.06.113

Cited by 6 publications

(2 citation statements)

References 36 publications

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“…Besides MBL-1, a recombinant bacterial BOA hydrolase (CbaA) from Pigmentiphaga sp. strain DL-8 is able to hydrolyse BOA into 2-AP ( Figure 1B) [23].…”

Section: Synthesis and Breakdown Of Bxs Are Divided Across Kingdomsmentioning

confidence: 99%

Allelopathic Plants: Models for Studying Plant–Interkingdom Interactions

Schandry

Becker

2020

Trends in Plant Science

107

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Allelopathy is a biochemical interaction between plants in which a donor plant releases secondary metabolites, allelochemicals, that are detrimental to the growth of its neighbours. Traditionally considered as bilateral interactions between two plants, allelopathy has recently emerged as a cross-kingdom process that can influence and be modulated by the other organisms in the plant's environment. Here, we review the current knowledge on plant-interkingdom interactions, with a particular focus on benzoxazinoids. We highlight how allelochemical-producing plants influence not only their plant neighbours but also insects, fungi, and bacteria that live on or around them. We discuss challenges that need to be overcome to study chemical plantinterkingdom interactions, and we propose experimental approaches to address how biotic and chemical processes impact plant health.

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“…Besides MBL-1, a recombinant bacterial BOA hydrolase (CbaA) from Pigmentiphaga sp. strain DL-8 is able to hydrolyse BOA into 2-AP ( Figure 1B) [23].…”

Section: Synthesis and Breakdown Of Bxs Are Divided Across Kingdomsmentioning

confidence: 99%

Allelopathic Plants: Models for Studying Plant–Interkingdom Interactions

Schandry

Becker

2020

Trends in Plant Science

107

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“…Pigmentiphaga strains are common inhabitants of polluted soil, activated sludge, and wastewater, and possess versatile metabolic capabilities for environmental pollutants, such as phenanthrene, aniline, acetamiprid, benzoxazolinone, and fenoxaprop‐ethyl (Blümel & Stolz, 2003; Dong et al, 2015; Dong et al, 2016; Dong et al, 2018; Huang et al, 2018; Li et al, 2018; Wang et al, 2013; Yang et al, 2020). In our previous study, a 3,5‐dibromo‐4‐hydroxybenzoate (DBHB)‐degrading strain of Pigmentiphaga sp.…”

Section: Introductionmentioning

confidence: 99%

Genetic redundancy of 4‐hydroxybenzoate 3‐hydroxylase genes ensures the catabolic safety of Pigmentiphaga sp. H8 in 3‐bromo‐4‐hydroxybenzoate‐contaminated habitats

Chen

Yang

et al. 2022

Environmental Microbiology

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Genetic redundancy is prevalent in organisms and plays important roles in the evolution of biodiversity and adaptation to environmental perturbation. However, selective advantages of genetic redundancy in overcoming metabolic disturbance due to structural analogues have received little attention. Here, functional divergence of the three 4-hydroxybenzoate 3-hydroxylase (PHBH) genes (phbh1~3) was found in Pigmentiphaga sp. strain H8. The genes phbh1/phbh2 were responsible for 3-bromo-4-hydroxybenzoate (3-Br-4-HB, an anthropogenic pollutant) catabolism, whereas phbh3 was primarily responsible for 4-hydroxybenzoate (4-HB, a natural intermediate of lignin) catabolism. 3-Br-4-HB inhibited 4-HB catabolism by competitively binding PHBH3 and was toxic to strain H8 cells especially at high concentrations. The existence of phbh1/phbh2 not only enabled strain H8 to utilize 3-Br-4-HB but also ensured the catabolic safety of 4-HB. Molecular docking and site-directed mutagenesis analyses revealed that Val199 and Phe384 of PHBH1/PHBH2 were required for the hydroxylation activity towards 3-Br-4-HB. Phylogenetic analysis indicated that phbh1 and phbh2 originated from a common ancestor and evolved specifically in strain H8 to adapt to 3-Br-4-HB-contaminated habitats, whereas phbh3 evolved independently. This study deepens our understanding of selective advantages of genetic redundancy in prokaryote's metabolic robustness and reveals the factors driving the divergent evolution of redundant genes in adaptation to environmental perturbation.

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Mass transfer and reaction simultaneously enhanced airlift microbial electrolytic cell system with high gaseous o-xylene removal capacity

Feng

et al. 2022

Chemosphere

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Expression and characterization of the key enzymes involved in 2-benzoxazolinone degradation by Pigmentiphaga sp. DL-8

Cited by 6 publications

References 36 publications

Allelopathic Plants: Models for Studying Plant–Interkingdom Interactions

Allelopathic Plants: Models for Studying Plant–Interkingdom Interactions

Genetic redundancy of 4‐hydroxybenzoate 3‐hydroxylase genes ensures the catabolic safety of Pigmentiphaga sp. H8 in 3‐bromo‐4‐hydroxybenzoate‐contaminated habitats

Mass transfer and reaction simultaneously enhanced airlift microbial electrolytic cell system with high gaseous o-xylene removal capacity

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