Expression and characterization of Nile tilapia (Oreochromis niloticus) secretory and membrane-bound IgM in response to bacterial infection

Yin, Xiaoxue; Mu, Liangliang; Fu, Shengli; Wu, Liting; Han, Kailiang; Wu, Haiyan; Bian, Xia; Wei, Xin; Guo, Zheng; Wang, An‐Li; Ye, Jianmin

doi:10.1016/j.aquaculture.2019.03.058

Cited by 32 publications

(21 citation statements)

References 75 publications

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“…Most of the literature in that area for teleost fish predates the discovery of IgT as the key mucosal immunoglobulin in these species (10). In that regard, some studies in several teleosts have reported low levels of pathogen-specific IgM titers upon bacterial infection (43,44). Moreover, whether the skin mucosa behaves as an inductive or effector site of mucosal Ig responses has never been investigated.…”

Section: Discussionmentioning

confidence: 99%

Prevailing Role of Mucosal Igs and B Cells in Teleost Skin Immune Responses to Bacterial Infection

Zhang

et al. 2021

The Journal of Immunology

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The skin of vertebrates is the outermost organ of the body and serves as the first line of defense against external aggressions. In contrast to mammalian skin, that of teleost fish lacks keratinization and has evolved to operate as a mucosal surface containing a skin-associated lymphoid tissue (SALT). Thus far, IgT representing the prevalent immunoglobulin (Ig) in SALT have only been reported upon infection with a parasite. However, very little is known about the types of B cells and Igs responding to bacterial infection in the teleost skin mucosa, as well as the inductive or effector role of the SALT in such responses. To address these questions, here we analyzed the immune response of trout skin upon infection with one of the most widespread fish skin bacterial pathogens, Flavobacterium columnare. This pathogen induced strong skin innate immune and inflammatory responses at the initial phases of infection. More critically, we found that the skin mucus of fish having survived the infection contained significant IgT-but not IgM-or IgD-specific titers against the bacteria. Moreover, we demonstrate the local proliferation and production of IgT + B-cells and specific IgT titers respectively within the SALT upon bacterial infection. Thus, our findings represent the first demonstration that IgT is the main Ig isotype induced by the skin mucosa upon bacterial infection, and that because of the large surface of the skin, its SALT probably represents a prominent IgT inductive site in fish..

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Section: Discussionmentioning

confidence: 99%

Prevailing Role of Mucosal Igs and B Cells in Teleost Skin Immune Responses to Bacterial Infection

Zhang

et al. 2021

The Journal of Immunology

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“…Complement 3 and 4 are the primary components of the complement system. IgM is the most important immunoglobulin in fish and is the first antibody to be produced when the body is stimulated by antigens (Yin et al, 2019). Studies have shown that exogenous induces an increase in complement content (Funk et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Effects of andrographolide on lipopolysaccharide‐induced serum biochemical indices, immune responses and intestinal inflammation related to gene expression of Monopterus albus

Shi

Zhong

et al. 2021

Aquaculture Research

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With the rapid expansion and intensification of the aquaculture industry, bacteria and viruses have become the most common stressors in aquatic animals. When fish are subjected to adverse stressors (e.g. crowding and invasion of bacteria and viruses), certain endocrine and physiological alterations occur, often resulting in changes in disease resistance and anti-inflammatory abilities (Chen, Chuang, et al., 2016;Chen, Guo, et al., 2016).Bacteria and viruses are among the most harmful pathogens to fish. Lipopolysaccharide (LPS), a gram-negative bacterial antigen, is often associated with immune responses and disease processes (Jiang et al., 2017). When LPS interacts with a corresponding receptor on the cell membrane, it activates an intracellular signalling chain, causing the activation of nuclear factor kappa-b (nfκb). This action initiates the gene transcription, expression

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“…Up-regulation of IgM mRNA in different organs post infection has been reported in different fish species like sea bass (Dicentrarchus labrax) 35 , turbot (Scophthalmus maximus) 36 , and rainbow trout (Oncorhynchus mykiss) 37 . In tilapia, Yin et al 38 showed IgM expression in various organs such as headkidney, spleen, thymus, liver, muscle, intestine, gill and skin post S. agalactiae infection, as early as 12 h post infection to highest levels by 5 dpc in the intestine and headkidney. We find that IgM levels were higher in target organs (liver and brain) than in lymphoid organs (headkidney and spleen).…”

Section: Discussionmentioning

confidence: 99%

Tilapia lake virus downplays innate immune responses during early stage of infection in Nile tilapia (Oreochromis niloticus)

Mugimba

Lamkhannat

Dubey

et al. 2020

Sci Rep

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Tilapia lake virus (TiLV) causes high mortality and high economic losses in tilapines. We describe an experimental challenge study focusing on early post challenge innate immune responses. Nile tilapia (Oreochromis niloticus) were infected with 105 TCID50/mL TiLV intraperitoneally, followed by virus quantification, histopathology and gene expression analysis in target (brain/liver) and lymphoid (spleen/headkidney) organs at 3, 7, 12, 17, and 34 days post challenge (dpc). Onset of mortality was from 21 dpc, and cumulative mortality was 38.5% by 34 dpc. Liver and kidney histopathology developed over the period 3–17 dpc, characterized by anisocytosis, anisokaryocytosis, and formation of multinucleated hepatocytes. Viral loads were highest at early time (3 dpc) in liver, spleen and kidney, declining towards 34 dpc. In brain, viral titer peaked 17 dpc. Innate sensors, TLRs 3/7 were inversely correlated with virus titer in brain and headkidney, and IFN-ß and Mx showed a similar pattern. All organs showed increased mRNA IgM expression over the course of infection. Overall, high virus titers downplay innate responses, and an increase is seen when viral titers decline. In silico modeling found that TiLV segments 4, 5 and 10 carry nucleolar localization signals. Anti-viral effects of TiLV facilitate production of virus at early stage of infection.

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Expression and characterization of Nile tilapia (Oreochromis niloticus) secretory and membrane-bound IgM in response to bacterial infection

Cited by 32 publications

References 75 publications

Prevailing Role of Mucosal Igs and B Cells in Teleost Skin Immune Responses to Bacterial Infection

Prevailing Role of Mucosal Igs and B Cells in Teleost Skin Immune Responses to Bacterial Infection

Effects of andrographolide on lipopolysaccharide‐induced serum biochemical indices, immune responses and intestinal inflammation related to gene expression of Monopterus albus

Tilapia lake virus downplays innate immune responses during early stage of infection in Nile tilapia (Oreochromis niloticus)

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