Expression and cellular localization of insulin-like growth factor 3 in gonads of the seasonal breeding teleost silver pomfret (Pampus argenteus)

Li, Yaya; Yang, Yang; Zhang, Youyi; Hu, Jiabao; Zhang, Man; Sun, Jiachu; Tian, Xinyue; Jin, Yuxuan; Zhang, Dingyuan; Wang, Yajun; Xu, Shanliang

doi:10.1007/s10695-022-01122-z

Cited by 3 publications

(7 citation statements)

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“…Currently, two types of Igfs (Igf1 and Igf2) have been identified in vertebrates [6, 7], and in teleost fish, gonad-specific Igf3 was recently found [8]. In our study, we also found that Igf3 was highly expressed during the gonadal development of silver pomfret [9], suggesting a unique role for Igfs in fish reproduction.…”

Section: Introductionsupporting

confidence: 76%

Insulin-like growth factor 1 promotes the gonadal development of Pampus argenteus by regulating energy metabolism

et al. 2023

Biology of Reproduction

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Insulin-like growth factor 1 (Igf1) is known to promote ovarian maturation by interacting with other hormones. However, the limited research on the role of Igf1 in the energy metabolism supply of gonads has hindered further exploration. To explore the role of Igf1 in gonadal development of silver pomfret, we analyzed the expression levels and the localization of igf1 mRNA and protein during testicular and ovarian development of silver pomfret. The results of the study showed upregulation of Igf1 in the critical period of vitellogenesis and sperm meiosis, which was found to be mainly expressed in the somatic cells of the gonads. Upon adding E2 and Igf1 to cultured gonadal tissues, expression of energy-related genes was significantly increased, along with the E2 enhanced effect of Igf1 in the testis. Importantly, stimulation of both ovaries and testes with E2 and Igf1 led to a remarkable increase in the expression of vitellogenesis and meiosis-related genes. Therefore, we conclude that Igf1 promotes vitellogenesis and sperm meiosis by regulating gonadal energy production. Moreover, the expression of Igf1 in gonads is significantly regulated by E2. These findings provide new insights for the research of Igf1 in fish breeding, thus allowing the regulation of energy metabolism between growth and reproduction for successful reproductive outcomes.

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Section: Introductionsupporting

confidence: 76%

Insulin-like growth factor 1 promotes the gonadal development of Pampus argenteus by regulating energy metabolism

et al. 2023

Biology of Reproduction

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“…Specific changes in ovary and germ cell development of P. argenteus in different photoperiod groups were observed under a light microscope (OLYMPUS‐BX60, Japan), and digital images were obtained using an imaging system (Olympus, DP72). The ovarian stage was determined by the germ cell phase with the highest average area ratio in the section (Y. Li et al ., 2022); then the ovarian stage of each sample was counted, and the percentage of each stage in each group was calculated.…”

Section: Methodsmentioning

confidence: 99%

“…Dissociation curve analysis was performed to determine target specificity, and the amplification efficiency was analysed. β‐actin, examined to be suitable for this study, was used as the internal control (Y. Li et al ., 2022). The relative expression was calculated by formula 2 −ΔΔCT .…”

Section: Methodsmentioning

confidence: 99%

“…The ovarian stage was determined by the germ cell phase with the highest average area ratio in the section (Y. Li et al, 2022); then the ovarian stage of each sample was counted, and the percentage of each stage in each group was calculated.…”

Section: Ovarian Histologymentioning

confidence: 99%

“…β-actin, examined to be suitable for this study, was used as the internal control (Y. Li et al, 2022). The relative expression was calculated by formula 2 ÀΔΔCT .…”

Section: Rna Isolation and Relative Gene Expression Analysismentioning

confidence: 99%

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Effects of different photoperiods on growth and ovarian development and maturation of silver pomfret Pampus argenteus

Wang

et al. 2023

Journal of Fish Biology

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Photoperiod has a great influence on the growth and ovarian development and maturation of fishes. To analyse the effects of photoperiod on growth and ovarian development of an important marine economic fish, silver pomfret Pampus argenteus, short photoperiod group (L:D = 8:16), control group (L:D = 12:12) and long photoperiod group (L:D = 18:6) were set up for 60 days. The growth performance, ovarian development, changes in hormones and key enzyme activities in the hypothalamic-pitui-taryÀgonadal (HPG) axis and expressions of key regulatory genes in the HPG axis were studied under different photoperiod conditions. The results showed that the final weight gain, body weight index, specific growth rate for weight, specific growth rate for length and average daily growth were the highest in the long photoperiod group, and the feed conversion rate was the lowest. Under long photoperiod condition, gonado-somatic index and hepato-somatic index were higher, ovarian maturity was better and expressions of HPG axisÀrelated regulatory genes foxl2a, foxl2b, cyp19a1a, cyp19a1b, kiss, gpr54-2, gnrh2, fsh and lh were higher. When compared with the other two groups, in the long photoperiod group, the change trend of estradiol (E2) was consistent with those of luteinizing hormone, melatonin (MT) and kisspeptin, and the levels were higher on the 20th and 50th days. These results indicate that prolongation of the photoperiod can improve the growth performance of P. argenteus and promote ovary development and maturation. The authors speculate that photoperiod may regulate the ovarian activity of P. argenteus through MT and kisspeptin/gpr54 signalling pathways. The results show that photoperiod can regulate the ovarian development of P. argenteus, which would help in breaking the seasonal restrictions of animals and regulating an animal's reproductive cycle.

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The Effect of Schizochytrium sp. on Growth, Fatty Acid Profile and Gut Microbiota of Silver Pomfret (Pampus argenteus)

Zhang

et al. 2023

JMSE

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Schizochytrium sp. is a kind of heterotrophic protist, rich in docosahexaenoic acid (DHA). To study the application value of Schizochytrium sp. in fish diet, a 20-day feeding experiment was initiated to evaluate its effect on growth, fatty acid contents, and the gut microbiota of the important economic fish, silver pomfret. In this study, the diets of the treatment group were added with 2% Schizochytrium sp. The fish in the treatment group gained more weight and had a higher growth rate than the control group. The levels of DHA, eicosapentaenoic acid, and polyunsaturated fatty acids in the edible tissue of fish were highly increased in the treatment group after 20 days. After feeding Schizochytrium sp., high-throughput 16S rRNA sequencing showed that the Proteobacteria, Firmicutes and Bacteroidetes were the phyla with the highest abundance, and at the genus and species level, we observed an increased abundance of probiotics. The results indicated that ingestion of Schizochytrium sp. could change the dominant microbiota population, which might lead to accelerated growth and improved unsaturated fatty acid content and fish health. This study provides a reference for Schizochytrium sp. supplementation in fish diets and could aid in the industrialization of silver pomfret.

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Expression and cellular localization of insulin-like growth factor 3 in gonads of the seasonal breeding teleost silver pomfret (Pampus argenteus)

Cited by 3 publications

References 56 publications

Insulin-like growth factor 1 promotes the gonadal development of Pampus argenteus by regulating energy metabolism

Insulin-like growth factor 1 promotes the gonadal development of Pampus argenteus by regulating energy metabolism

Effects of different photoperiods on growth and ovarian development and maturation of silver pomfret Pampus argenteus

The Effect of Schizochytrium sp. on Growth, Fatty Acid Profile and Gut Microbiota of Silver Pomfret (Pampus argenteus)

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