Expression and biochemical characterization of a Bacillus subtilis catalase in Pichia pastoris X-33

Jiang, Mengtong; Liu, Yuxin; Hou, Xue; Wang, Yiqi; Wang, Conggang; Yang, Fan; Li, Xianzhen

doi:10.1016/j.pep.2023.106277

Cited by 2 publications

(2 citation statements)

References 42 publications

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“…RCAT protein concentrations were measured by BCA protein assay kit, and its activity was determined by the hydrogen peroxide method [ 13 ]. Specific steps are enumerated in S1 (Supplementary file) .…”

Section: Methodsmentioning

confidence: 99%

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Degradation Mechanism of Aflatoxin M1 by Recombinant Catalase from Bacillus pumilusE-1-1-1: Food Applications in Milk and Beer

Liu,

Zhao,

Wang

et al. 2024

Foods

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A bacteria capable of degrading aflatoxin M1 (AFM1) was isolated from African elephant manure. It was identified as Bacillus pumilus by 16s rDNA sequencing and named B. pumilusE-1-1-1. Compared with physical and chemical methods, biological methods have attracted much attention due to their advantages, such as thorough detoxification, high specificity, and environmental friendliness. This work aimed to study the effects of a recombinant catalase (rCAT) from B. pumilusE-1-1-1 on the degradation of AFM1 in pattern solution. The degradation mechanism was further explored and applied to milk and beer. Kinetic Momentum and Virtual Machine Maximum values for rCAT toward AFM1 were 4.1 μg/mL and 2.5 μg/mL/min, respectively. The rCAT-mediated AFM1 degradation product was identified as C15H14O3. Molecular docking simulations suggested that hydrogen and pi bonds played major roles in the steadiness of AFM1–rCAT. In other work, compared with identical density of AFM1, survival rates of Hep-G2 cells incubated with catalase-produced AFM1 degradation products increased by about 3 times. In addition, degradation rates in lager beer and milk were 31.3% and 47.2%, respectively. Therefore, CAT may be a prospective substitute to decrease AFM1 contamination in pattern solution, milk, and beer, thereby minimizing its influence on human health.

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Section: Methodsmentioning

confidence: 99%

“…It has been reported that a rCAT from Corynebacterium glutamicum exhibited its highest activity at 30 °C and represented good thermal stability from 25 °C to 50 °C [21]. Philibert et al reported that a new recombinant heme-catalase from B. subtilis 168, with an optimum temperature of 40 °C, improved oxidative stress in B. subtilis [22].…”

Section: Temperaturementioning

confidence: 99%

Degradation Mechanism of Aflatoxin M1 by Recombinant Catalase from Bacillus pumilusE-1-1-1: Food Applications in Milk and Beer

Liu,

Zhao,

Wang

et al. 2024

Foods

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Antimicrobial Efficacy of Photocaged β‐Lapachone in Bacillus subtilis Biofilms

Hudson,

Faylinn,

Lopez‐Miranda

et al. 2024

ChemPhotoChem

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With the rise of antibiotic resistance within clinical settings, combating the growth of microbial biofilms presents a unique challenge. Biofilm‐inhabiting bacteria are embedded within a self‐produced, protective matrix, which can reduce the efficacy of treatment. The naturally derived product β‐lapachone is an appealing therapeutic agent that has been reported to inhibit biofilm growth. However, its off‐target toxicity and poor metabolic stability pose a significant hurdle for its application in vivo. Using a photo‐pharmacological approach via a coumarin‐based photocage, the reactivity of β‐lapachone can be tuned so it only becomes active once the photocage is removed. Here we report both the photo‐uncaging efficiency and the effective inhibition concentration of photocaged β‐lapachone within model Bacillus subtilis biofilms. Additionally, the mechanism of action is analyzed with results supporting catalase inhibition. This novel light‐activatable anti‐microbial has potential applications in medical settings to inhibit biofilm growth and provide synergistic treatment with traditional antibiotics.

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Expression and biochemical characterization of a Bacillus subtilis catalase in Pichia pastoris X-33

Cited by 2 publications

References 42 publications

Degradation Mechanism of Aflatoxin M1 by Recombinant Catalase from Bacillus pumilusE-1-1-1: Food Applications in Milk and Beer

Degradation Mechanism of Aflatoxin M1 by Recombinant Catalase from Bacillus pumilusE-1-1-1: Food Applications in Milk and Beer

Antimicrobial Efficacy of Photocaged β‐Lapachone in Bacillus subtilis Biofilms

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