Expression and Biochemical Characterization of a Novel Fucoidanase from Flavobacteriumalgicola with the Principal Product of Fucoidan-Derived Disaccharide

Abstract

Fucoidan is one of the main polysaccharides of brown algae and echinoderm, which has nutritional and pharmacological functions. Due to the low molecular weight and exposure of more sulfate groups, oligo-fucoidan or fucoidan oligosaccharides have potential for broader applications. In this research, a novel endo-α-1,4-L-fucoidanase OUC-FaFcn1 which can degrade fucoidan into oligo-fucoidan was discovered from the fucoidan-digesting strain Flavobacterium algicola 12,076. OUC-FaFcn1 belongs to glycoside hydrolases (GH) family 107 and shows highest activity at 40 °C and pH 9.0. It can degrade the α-1,4 glycosidic bond, instead of α-1,3 glycosidic bond, of the fucoidan with a random tangent way to generate the principal product of disaccharide, which accounts for 49.4% of the total products. Therefore, OUC-FaFcn1 is a promising bio-catalyst for the preparation of fucoidan-derived disaccharide. These results further enrich the resource library of fucoidanase and provide the basis for the directional preparation of fucoidan-derived oligosaccharide with specific polymerization.

Purification and Characterization of the Enzyme Fucoidanase from Cobetia amphilecti Utilizing Fucoidan from Undaria pinnatifida

“…Several studies have suggested that LMWF exhibits biological activities such as anti-inflammatory [ 12 ], antiangiogenic [ 13 ], a hypoglycemic effect [ 14 ] and antioxidant activity [ 15 , 16 ]. In addition, LMWF has also been shown to have a protective effect against hypertension, hyperlipidemia and the hyper-responsiveness of aortic smooth muscle in type I diabetic rats [ 17 ]. LMWF has great potential as an adjuvant treatment for cardiovascular complications in type I diabetes mellitus [ 18 ].…”

“…[ 36 ], Patinopecten (Mizuhopecten) yessoensis [ 37 ] and marine bacteria such as Fucobacter marina SI-0098 ( Flavobacterium sp. SA-0082) [ 38 ], Flavobacterium algicola [ 17 ] and Vibrio sp. Other groups of marine organisms in which fucoidanase occurs are N-5 [ 39 ], marine proteobacteria such as Pseudoaltero monascitrea KMM 3296, KMM 3297 and KMM 3298 [ 28 ], Alteromonas sp.…”

Fucanases Related to the GH107 Family from Members of the PVC Superphylum

“…In particular, the GH107 family contains only 38 members from bacterial isolates of the Bacteroidota and Pseudomonadota phyla, from a candidate genome of a Gammaproteobacterium [22], and one member from the diatom Thalassiosira oceanica CCMP1005, according to the CAZy database (November 2023). Most of the characterized enzymes present endo-α-(1,4)-L-fucanase activity (EC 3.2.1.212) [17,[22][23][24][25][26][27], while Mef2 from Allomuricauda eckloniae [28] and the enzymes Fda1 and Fda2 from Alteromonas sp. SN-1009 were reported as presenting endo-α-(1,3)-L-fucanase activity (EC 3.2.1.211) [29,30].…”

The Discovery of the Fucoidan-Active Endo-1→4-α-L-Fucanase of the GH168 Family, Which Produces Fucoidan Derivatives with Regular Sulfation and Anticoagulant Activity

“…Fucoidanase solutions (10 µL with concentration of 0.01 mg/mL) in 0.04 M Tris-HCl buffer (pH 6.8) with 100 mM NaCl were incubated with 10 µL of the fucoidan solution (10 mg/mL) in water at different temperatures (25,30,35,40,45,50,55,60,65, and 70 • C) for 1 h. Enzyme activity was monitored as described above.…”

“…At present, several fucoidan-active α-L-fucosidases (cleavage of terminal L-fucose residues in fucoidans and/or fucooligosaccharides) [ 14 , 15 ], sulfatases (elimination of sulfate groups from sulfated L-fucose residues in fucoidans and/or fucooligosaccharides) [ 16 , 17 , 18 ], and endo-fucanases (syn. fucoidanases or fucoidan hydrolases) have been described [ 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ].…”