Expression and association of carbonic anhydrase IX and cyclooxygenase-2 in colorectal cancer

Tupá, Veronika; Drahošová, Slávka; Grendár, Marian; Adamkov, Marián

doi:10.1016/j.prp.2019.01.012

Cited by 10 publications

(6 citation statements)

References 48 publications

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“…Furthermore, the rate of absent CAIX staining was comparable to other gastrointestinal adenocarcinomas. 27,64,65 Total negativity of staining was not related to stage or prognosis (data not shown).…”

Section: Discussionmentioning

confidence: 93%

“…Regarding colorectal cancer, several groups have found variable levels of CAIX expression, but no significant association with clinicopathological variables or survival. 42,60,64,69 However, many investigators still maintain that pharmacological inhibition of CAIX or knockout of CA9 reduce colorectal cancer cell growth, at least in hypoxic conditions. [70][71][72] The oncological treatment protocols for colorectal cancer are currently adopted for the treatment of DAC.…”

Section: Discussionmentioning

confidence: 99%

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Carbonic Anhydrases II and IX in Non-ampullary Duodenal Adenomas and Adenocarcinoma

Nortunen

Parkkila

Saarnio

et al. 2021

J Histochem Cytochem.

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Non-ampullary duodenal adenocarcinoma (DAC) is a rare malignancy. Little information is available concerning the histopathological prognostic factors associated with DAC. Carbonic anhydrases (CAs) are metalloenzymes catalyzing the universal reaction of CO2 hydration. Isozymes CAII, CAIX, and CAXII are associated with prognosis in various cancers. Our aim was to analyze the immunohistochemical expressions of CAII, CAIX, and CAXII in normal duodenal epithelium, duodenal adenomas, and adenocarcinoma and their associations with clinicopathological variables and survival. Our retrospective study included all 27 DACs treated in Oulu University Hospital during years 2000–2020. For comparison, samples of 42 non-ampullary adenomas were collected. CAII expression was low in duodenal adenomas and adenocarcinoma. CAIX expression in adenomas and adenocarcinoma was comparable with the high expression of normal duodenal crypts. Expression patterns in carcinomas were largely not related to clinicopathological features. However, low expression of CAII associated with poorer differentiation of the tumor ( p=0.049) and low expression of CAIX showed a trend for association with nodal spread, although statistical significance was not reached ( p=0.091). CAII and CAIX lost their epithelial polarization and staining intensity in adenomas. CAXII expression was not detected in the studied samples. CAs were not associated with survival. The prognostic value of CAII and CAIX downregulation should be further investigated. Both isozymes may serve as biomarkers of epithelial dysplasia in the duodenum. (J Histochem Cytochem XX: XXX–XXX, XXXX)

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Carbonic Anhydrases II and IX in Non-ampullary Duodenal Adenomas and Adenocarcinoma

Nortunen

Parkkila

Saarnio

et al. 2021

J Histochem Cytochem.

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“…CAIX correlated with CD163+ tumor-associated macrophages in cervical cancer 31 . Previous studies confirmed association of CAIX and pro-inflammatory enzyme cyclooxygenase-2 (COX-2) in patients with colorectal cancer 32 . High-risk HPV and CAIX were independent risk factors for head and neck neoplasia 33 .…”

Section: Introductionmentioning

confidence: 72%

Aerobic vaginitis is associated with carbonic anhydrase IX in cervical intraepithelial neoplasia

Grincevičienė,

Vaitkienė,

Kanopienė

et al. 2024

Sci Rep

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The aim of this study was to analyze the association between vaginal microbiota, carbonic anhydrase IX (CAIX) and histological findings of cervical intraepithelial neoplasia (CIN). The study included 132 females, among them 66 were diagnosed with high-grade intraepithelial lesion (CIN2, CIN3, and cancer), 14 with low-grade disease, and 52 assigned to the control group. An interview focused on the behavior risk factors, together with vaginal fluid pH measurement, wet mount microscopy, detection of Chlamydia trachomatis, and Trichomonas vaginalis were performed. After colposcopy, high-grade abnormalities were detected via direct biopsies and treated with conization procedure. Conuses were immuno-stained with CAIX antibody. The histological findings were CIN1 (n = 14), and CIN2+ (included CIN2 (n = 10), CIN3 (n = 49), and cancer (n = 7; squamous cell carcinomas)). Prevalence of bacterial vaginosis (BV) was similar between the groups. Moderate or severe aerobic vaginitis (msAV) was diagnosed more often among CIN2+ (53.0%) than CIN1 (21.4%). Moderate or strong immunostaining of CAIX (msCAIX) was not detected among CIN1 cases. Thus, msAV was prevalent in CAIX non-stained group (p = 0.049) among CIN2 patients. Co-location of msAV and msCAIX was found in CIN3. Regression model revealed that msAV associated with high-grade cervical intraepithelial neoplasia independently from smoking and the number of partners.

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“…The human (h)CA IX is an example of a tumour-associated CA isoform, with several groups associating it with tumour activity, cell proliferation, cell adhesion and malignant cell invasion [ 16 , 17 ], being thus also commonly considered as a potential biomarker for tumour diagnosis [ 18 ]. More specifically, hCA IX’s overexpression levels and increased activity have been recently associated with higher rates of hypoxia survival and cell invasion in tumours [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

“…Associated with CA IX action and overexpression in hypoxic and invasive tumours, such as CRC [ 18 , 19 ], is also the commonly overexpressed epithelial growth factor receptor (EGFR), which mediates cancer initiation, progression and proliferation [ 29 , 30 ]. This association makes anti-EGFR agents also good targeting probes, with several works reporting the conjugated use of several anti-EGFR antibodies, such as the commercial Cetuximab (CTX), with therapeutic molecules (drugs, pro-drugs, etc.)…”

Section: Introductionmentioning

confidence: 99%

Development of New Targeted Nanotherapy Combined with Magneto-Fluorescent Nanoparticles against Colorectal Cancer

Marcelo

Montpeyó

Galhano

et al. 2023

IJMS

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The need for non-invasive therapies capable of conserving drug efficiency and stability while having specific targetability against colorectal cancer (CRC), has made nanoparticles preferable vehicles and principal building blocks for the development of complex and multi-action anti-tumoral approaches. For that purpose, we herein report the production of a combinatory anti-tumoral nanotherapy using the production of a new targeting towards CRC lines. To do so, Magneto-fluorescent NANO3 nanoparticles were used as nanocarriers for a combination of the drugs doxorubicin (DOX) and ofloxacin (OFLO). NANO3 nanoparticles’ surface was modified with two different targeting agents, a newly synthesized (anti-CA IX acetazolamide derivative (AZM-SH)) and a commercially available (anti-epidermal growth factor receptor (EGFR), Cetuximab). The cytotoxicity revealed that only DOX-containing nanosystems showed significant and even competitive cytotoxicity when compared to that of free DOX. Interestingly, surface modification with AZM-SH promoted an increased cellular uptake in the HCT116 cell line, surpassing even those functionalized with Cetuximab. The results show that the new target has high potential to be used as a nanotherapy agent for CRC cells, surpassing commercial targets. As a proof-of-concept, an oral administration form of NANO3 systems was successfully combined with Eudragit® enteric coating and studied under extreme conditions.

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Expression and association of carbonic anhydrase IX and cyclooxygenase-2 in colorectal cancer

Cited by 10 publications

References 48 publications

Carbonic Anhydrases II and IX in Non-ampullary Duodenal Adenomas and Adenocarcinoma

Carbonic Anhydrases II and IX in Non-ampullary Duodenal Adenomas and Adenocarcinoma

Aerobic vaginitis is associated with carbonic anhydrase IX in cervical intraepithelial neoplasia

Development of New Targeted Nanotherapy Combined with Magneto-Fluorescent Nanoparticles against Colorectal Cancer

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