Expression and Analysis of Green Fluorescent Proteins in Human Embryonic Kidney Cells by Capillary Electrophoresis

Malek, Amir; Khaledi, Morteza G.

doi:10.1006/abio.1998.2975

Cited by 28 publications

(25 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present paper we chose to use the classical Ca-phosphate method as a transfection procedure because of its universality and simplicity. In agreement with others (29), when HEK cells were transfected with undamaged pEGFP or pEYFP, the fluorescent signal gradually increased in intensity during the first 24 h after transfection and then remained constant up to 72 h (not shown). The ratio of the fluorescence of the EGFP to that of EYFP (R) also remained constant during this period (Fig.…”

Section: Expression Of Egfp In Hek293 Cellssupporting

confidence: 89%

Two-Wavelength Fluorescence Assay for DNA Repair

Roguev

Russev

2000

Analytical Biochemistry

View full text Add to dashboard Cite

Section: Expression Of Egfp In Hek293 Cellssupporting

confidence: 89%

Two-Wavelength Fluorescence Assay for DNA Repair

Roguev

Russev

2000

Analytical Biochemistry

View full text Add to dashboard Cite

“…The small volume of the analyte needed in CE experiments has made possible the measurement of the cellular contents (native proteins [357], specific fluorescent-labelled or-induced proteins [358], metabolites), the monitoring of single cells [359,360] or bacteria communications [361]. Different CE-techniques like CGE or CIEF were developed over the last years and are currently used intensively in a similar manner to classical gel electrophoresis [362] in the fields of human genomics and proteomics [363,364].…”

Section: Discussionmentioning

confidence: 99%

Capillary electrophoresis – mass spectrometry: 15 years of developments and applications

Schmitt‐Kopplin¹,

Frommberger²

2003

Electrophoresis

282

232

View full text Add to dashboard Cite

Capillary electrophoresis -mass spectrometry: 15 years of developments and applicationsSince its introduction in 1987, capillary electrophoresis-mass spectrometry (CE-MS) has developed to a well accepted multidimensional analytical approach complementary and/or competitive to classical MS-hyphenated separation techniques. The threefold combination of rapid developments of an exceptional separation technique, of selective mass detection possibilities, and of very mild ionization modes first allowed these progresses. This article shows the CE specificities that need to be well controlled/known, compared to classical and more routinely used liquid chromatography in the light of its coupling to MS. The major trends and developments over the last 15 years and most of the reviews and applications found in ISI Web of science and publisher databases are presented in a tabulated way. The reader can thus rapidly find existing CE-MS analysis techniques in his field of research and application (forensics, environment, bioanalytics, pharmaceutics, and metabolites).

show abstract

“…For example, Fig. 7A is an electropherogram showing the separation of S65T GFP from autofluorescent media impurities, in the analysis of an extract of human embryonic kidney (HEK293) cells, from the work of Malek and Khaledi (54). These workers have also reported the quantification of GFP in whole HEK293 cells using CE, without the need for cell lysis, as shown in Fig.…”

Section: Capillary Electrophoresismentioning

confidence: 94%