Expression and activity of AIM2-inflammasome in rheumatoid arthritis patients

Méndez-Frausto, Gwendolyne; Medina-Rosales, Marina Nayeli; Uresti-Rivera, Edith Elena; Baranda-Cándido, Lourdes; Zapata-Zúñiga, Martín; Bastián, Yadira; Amaro, Roberto González; Enciso-Moreno, José Antonio; García-Hernández, Mariana Haydee

doi:10.1016/j.imbio.2019.11.015

Cited by 11 publications

(9 citation statements)

References 33 publications

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“…However, the AIM2 expression levels were lower in the group of patients with DAS28 > 4 than in the control group, especially in late-stage disease (>2 years). These results showed that monocytes from patients with RA were prone to release IL-1β in the absence of the AIM2 inflammasome signal [87]. Chen et al also reported that AIM2 expression in RA sera was lower than that in HC sera.…”

Section: Aim2mentioning

confidence: 88%

Cell-Free DNA in Rheumatoid Arthritis

Hashimoto

Yoshida

Hashiramoto

2021

IJMS

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Endogenous DNA derived from the nuclei or mitochondria is released into the bloodstream following cell damage or death. Extracellular DNA, called cell-free DNA (cfDNA), is associated with various pathological conditions. Recently, multiple aspects of cfDNA have been assessed, including cfDNA levels, integrity, methylation, and mutations. Rheumatoid arthritis (RA) is the most common form of autoimmune arthritis, and treatment of RA has highly varied outcomes. cfDNA in patients with RA is elevated in peripheral blood and synovial fluid and is associated with disease activity. Profiling of cfDNA in patients with RA may then be utilized in various aspects of clinical practice, such as the prediction of prognosis and treatment responses; monitoring disease state; and as a diagnostic marker. In this review, we discuss cfDNA in patients with RA, particularly the sources of cfDNA and the correlation of cfDNA with RA pathogenesis. We also highlight the potential of analyzing cfDNA profiles to guide individualized treatment approaches for RA.

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Section: Aim2mentioning

confidence: 88%

Cell-Free DNA in Rheumatoid Arthritis

Hashimoto

Yoshida

Hashiramoto

2021

IJMS

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“…Furthermore, AIM2 expression is upregulated in oral, cervical, and lung cancer and downregulated in colorectal and small bowel cancer (166). AIM2 expression is protective in rheumatoid arthritis patients, leading to higher IL-1b release in the absence of AIM2 (185).…”

Section: Yes (163)mentioning

confidence: 99%

Cannabinoids as Key Regulators of Inflammasome Signaling: A Current Perspective

Suryavanshi

Kovalchuk

2021

Front. Immunol.

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Inflammasomes are cytoplasmic inflammatory signaling protein complexes that detect microbial materials, sterile inflammatory insults, and certain host-derived elements. Inflammasomes, once activated, promote caspase-1–mediated maturation and secretion of pro-inflammatory cytokines, interleukin (IL)-1β and IL-18, leading to pyroptosis. Current advances in inflammasome research support their involvement in the development of chronic inflammatory disorders in contrast to their role in regulating innate immunity. Cannabis (marijuana) is a natural product obtained from the Cannabis sativa plant, and pharmacologically active ingredients of the plant are referred to as cannabinoids. Cannabinoids and cannabis extracts have recently emerged as promising novel drugs for chronic medical conditions. Growing evidence indicates the potent anti-inflammatory potential of cannabinoids, especially Δ9-tetrahydrocannabinol (Δ9-THC), cannabidiol (CBD), and synthetic cannabinoids; however, the mechanisms remain unclear. Several attempts have been made to decipher the role of cannabinoids in modulating inflammasome signaling in the etiology of chronic inflammatory diseases. In this review, we discuss recently published evidence on the effect of cannabinoids on inflammasome signaling. We also discuss the contribution of various cannabinoids in human diseases concerning inflammasome regulation. Lastly, in the milieu of coronavirus disease-2019 (COVID-19) pandemic, we confer available evidence linking inflammasome activation to the pathophysiology of COVID-19 suggesting overall, the importance of cannabinoids as possible drugs to target inflammasome activation in or to support the treatment of a variety of human disorders including COVID-19.

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“…It is surprisingly to observe the decreased levels of AIM2 in RA than HC in our study. Recently, Mendez-Frausto et al [ 26 ] observed that AIM2 was reduced at a systemic level in patients with RA, and the monocytes of RA patients were found to be prone to releasing IL-1 β in the absence of AIM2 inflammasome signals. These findings were in accordance with our results of ELISA; however, reasons regarding the decreased levels of AIM2 according to disease activity in serum remain unclear.…”

Section: Discussionmentioning

confidence: 99%

Expression of AIM2 in Rheumatoid Arthritis and Its Role on Fibroblast-Like Synoviocytes

Chen

Qiu

Chen

et al. 2020

Mediators of Inflammation

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Objectives. To determine differences in AIM2 inflammasome expression levels between rheumatoid arthritis (RA) and osteoarthritis (OA) and to investigate the role of AIM2 in RA fibroblast-like synoviocytes (RA-FLS). Methods. Serum AIM2 levels among health controls (HC, n = 20 ), OA ( n = 25 ), and RA (n =49) patients were compared via ELISA. The different expression levels of AIM2, ASC, caspase-1, and IL-1β between RA and OA synovium were semiquantified by qRT-PCR and immunohistochemical (IHC) staining. IHC staining was recorded by H scores, and its correlation with the ESR and CRP levels of RA patients was determined. SiRNA AIM2 was transferred to RA-FLS and its effects on the proliferation and migration via CCK-8 assay and Transwell test, respectively. Results. In RA sera, the HC expressed higher level of AIM2 than OA and RA patients, and ASC, caspase-1, and IL-1β expressed higher in RA patients than HC; no significant differences were observed between sera of OA and RA patients. However, in affected knee synovium, AIM2, ASC, caspase-1, and IL-1β were expressed higher in RA than that of OA. Moreover, the H scores of AIM2, ASC, and IL-1β were positively correlated with the ESR and CRP levels in RA patients. The proliferation of FLS was significantly inhibited after transferring with AIM2 siRNA to FLS. There were no differences in apoptosis and migration assay between the si-AIM2 group and the control group. Conclusion. AIM2 inflammasome pathway involves in the pathogenesis of RA. si-AIM2 inhibits the proliferation of RA-FLS, which may be a promising therapeutic strategy for the treatment of RA.

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Expression and activity of AIM2-inflammasome in rheumatoid arthritis patients

Cited by 11 publications

References 33 publications

Cell-Free DNA in Rheumatoid Arthritis

Cell-Free DNA in Rheumatoid Arthritis

Cannabinoids as Key Regulators of Inflammasome Signaling: A Current Perspective

Expression of AIM2 in Rheumatoid Arthritis and Its Role on Fibroblast-Like Synoviocytes

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