Expression analysis of two rice pollen-specific promoters using homologous and heterologous systems

Nguyễn, Tiến Dũng; Oo, Moe Moe; Moon, Sunok; Bae, Hyun-Kyung; Oh, Sung Aeong; Soh, Moon-Soo; Song, Jong Tae; Kim, Jeong Hoe; Jung, Ki‐Hong; Park, Soon Ki

doi:10.1007/s11816-015-0365-4

Cited by 5 publications

(5 citation statements)

References 45 publications

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“…In the process of constructing synthetic promoters, these factors should be concerned with different degrees to meet individual demands. Consequently, we selected ten expression regulatory sequences based on a statistical results of relevant existing reports (Cai et al ., ; Carlos et al ., ; Gianì et al ., ; Huang, ; Jeong and Jung, ; Kuwano et al ., ; Lin et al ., ; Lu et al ., ; Manikandan et al ., ; Molla et al ., ; Nguyen et al ., ; Park et al ., ; Wang et al ., ; Xu et al ., ; Yang et al ., ; Zhang et al ., ). The four features of each expression regulatory sequence (tissue specificity, expression efficiency, sequence length and universality) were, respectively, scored based on our scoring standard, as shown in Table S1 in detail.…”

Section: Methodsmentioning

confidence: 98%

Comprehensive construction strategy of bidirectional green tissue‐specific synthetic promoters

Bai

Wang

et al. 2019

Plant Biotechnology Journal

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Summary Bidirectional green tissue‐specific promoters have important application prospects in genetic engineering and crop genetic improvement. However, there is no report on the application of them, mainly due to undiscovered natural bidirectional green tissue‐specific promoters and the lack of a comprehensive approach for the synthesis of these promoters. In order to compensate for this vacancy, the present study reports a novel strategy for the expression regulatory sequence selection and the bidirectional green tissue‐specific synthetic promoter construction. Based on this strategy, seven promoters were synthesized and introduced into rice by agrobacterium‐mediated transformation. The functional identification of these synthetic promoters was performed by the expression pattern of GFP and GUS reporter genes in two reverse directions in transgenic rice. The results indicated that all the synthetic promoters possessed bidirectional expression activities in transgenic rice, and four synthetic promoters (BiGSSP2, BiGSSP3, BiGSSP6, BiGSSP7) showed highly bidirectional expression efficiencies specifically in green tissues (leaf, sheath, panicle, stem), which could be widely applied to agricultural biotechnology. Our study provided a feasible strategy for the construction of synthetic promoters, and we successfully created four bidirectional green tissue‐specific synthetic promoters. It is the first report on bidirectional green tissue‐specific promoters that could be efficiently applied in genetic engineering.

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Section: Methodsmentioning

confidence: 98%

Comprehensive construction strategy of bidirectional green tissue‐specific synthetic promoters

Bai

Wang

et al. 2019

Plant Biotechnology Journal

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“…Recently, we reported the activity of the promoters of 11 mature pollen-specific genes, OsLPS1 to OsLPS11, in rice. Seven of the these promoters were also operated in a heterologous system using Arabidopsis (Nguyen et al 2015;Oo et al 2014). One promoter, OsLPS10 which was showed expression at bi-nucleated microspore stage, was successfully used for the complementation of Arabidopsis gametophytic mutant which was defective in microspore development prior to PMI (Nguyen et al 2015).…”

Section: Meta-expression Analysis For Genome-wide Identification Of Rmentioning

confidence: 99%

“…As shown in Fig. 4a, eight vector constructs were prepared and then transformed into rice callus mediated by Agrobacterium tumefaciens LBA4404 strains (Nguyen et al 2015). As a result, in total, 316 putative transgenic lines were generated with eight constructs.…”

Section: Expression Patterns Of Microspore-preferred Genes Were Confimentioning

confidence: 99%

“…To date, many genes that are expressed in anther or pollen tissues have been identified in rice. Based on expression patterns, they were grouped as pollen mother cell or tapetum-expressed genes including TDR, Osg6B, RA8, RTS, UDT1, carbon starved anther (CSA), OsLTP 6, MTR1, MEL1 and ZIP4 (Yokoi et al 1997;Jeon et al 1999;Jung et al 2005;Luo et al 2006;Li et al 2006;Zhang et al 2010a;Shen et al 2012;Liu et al 2013;Niu et al 2013;Komiya et al 2014) and late pollen-expressed genes including OsSCP1, OsSCP2, OsSCP3, RIP1, OSIPA, OSIPK, OsLPS1-9, OsLSP10 and OsLPS11 (Park et al 2006;Han et al 2006;Gupta et al 2007;Swapna et al 2011;Khurana et al 2013;Oo et al 2014;Nguyen et al 2015). However, a few rice microspore-specific or preferred genes such as OsCP1 and OsPH1;2 have been functionally studied (Lee et al 2004;Secco et al 2010).…”

Section: Role Of Pollen-specific Cres Identified In Promoters Of Rmp mentioning

confidence: 99%

“…Pre-cultured seeds were transformed via Agrobacterium tumefaciens (LBA4404) as described by Nguyen et al (2015). Pre-cultured seeds were inoculated with Agrobacteria suspension harboring proRMP::GFP-GUS for 2 min with gentle inversion.…”

Section: Plant Transformation and Selection Of Transgenic Plantsmentioning

confidence: 99%

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Genome-wide identification and analysis of rice genes preferentially expressed in pollen at an early developmental stage

et al. 2016

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Microspore production using endogenous developmental programs has not been well studied. The main limitation is the difficulty in identifying genes preferentially expressed in pollen grains at early stages. To overcome this limitation, we collected transcriptome data from anthers and microspore/pollen and performed meta-expression analysis. Subsequently, we identified 410 genes showing preferential expression patterns in early developing pollen samples of both japonica and indica cultivars. The expression patterns of these genes are distinguishable from genes showing pollen mother cell or tapetum-preferred expression patterns. Gene Ontology enrichment and MapMan analyses indicated that microspores in rice are closely linked with protein degradation, nucleotide metabolism, and DNA biosynthesis and regulation, while the pollen mother cell or tapetum are strongly associated with cell wall metabolism, lipid metabolism, secondary metabolism, and RNA biosynthesis and regulation. We also generated transgenic lines under the control of the promoters of eight microspore-preferred genes and confirmed the preferred expression patterns in plants using the GUS reporting system. Furthermore, cis-regulatory element analysis revealed that pollen specific elements such as POLLEN1LELAT52, and 5659BOXLELAT5659 were commonly identified in the promoter regions of eight rice genes with more frequency than estimation. Our study will provide new sights on early pollen development in rice, a model crop plant.

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Application of rice microspore-preferred promoters to manipulate early pollen development in Arabidopsis: a heterologous system

Nguyễn

Moon

et al. 2016

Plant Reprod

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Rice microspore-promoters. Based on microarray data analyzed for developing anthers and pollen grains, we identified nine rice microspore-preferred (RMP) genes, designated RMP1 through RMP9. To extend their biotechnological applicability, we then investigated the activity of RMP promoters originating from monocotyledonous rice in a heterologous system of dicotyledonous Arabidopsis. Expression of GUS was significantly induced in transgenic plants from the microspore to the mature pollen stages and was driven by the RMP1, RMP3, RMP4, RMP5, and RMP9 promoters. We found it interesting that, whereas RMP2 and RMP6 directed GUS expression in microspore at the early unicellular and bicellular stages, RMP7 and RMP8 seemed to be expressed at the late tricellular and mature pollen stages. Moreover, GUS was expressed in seven promoters, RMP3 through RMP9, during the seedling stage, in immature leaves, cotyledons, and roots. To confirm microspore-specific expression, we used complementation analysis with an Arabidopsis male-specific gametophytic mutant, sidecar pollen-2 (scp-2), to verify the activity of three promoters. That mutant shows defects in microspore development prior to pollen mitosis I. These results provide strong evidence that the SIDECAR POLLEN gene, driven by RMP promoters, successfully complements the scp-2 mutation, and they strongly suggest that these promoters can potentially be applied for manipulating the expression of target genes at the microspore stage in various species.

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Expression analysis of two rice pollen-specific promoters using homologous and heterologous systems

Cited by 5 publications

References 45 publications

Comprehensive construction strategy of bidirectional green tissue‐specific synthetic promoters

Comprehensive construction strategy of bidirectional green tissue‐specific synthetic promoters

Genome-wide identification and analysis of rice genes preferentially expressed in pollen at an early developmental stage

Application of rice microspore-preferred promoters to manipulate early pollen development in Arabidopsis: a heterologous system

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