Expression analysis of the Toll-like receptor and TIR domain adaptor families of zebrafish

Meijer, Annemarie H.; Krens, S.F Gabby; Rodriguez, Indira A Medina; He, Shuning; Bitter, Wilbert; Snaar-Jagalska, B. Ewa; Spaink, Herman P.

doi:10.1016/j.molimm.2003.10.003

Cited by 482 publications

(336 citation statements)

References 44 publications

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“…Some researchers have speculated that most fish lost tlr4 genes during evolution (Oshiumi et al, 2003;Baoprasertkul et al, 2007), and to date, tlr4 has only been found in cyprinids (Meijer et al, 2004;Jault et al, 2004;Su et al, 2009). The zebrafish tlr4 cluster contains three genes, while the grass carp tlr4 cluster contains four genes, which suggests that fish tlr4 genes have actively evolved.…”

Section: Discussionmentioning

confidence: 99%

“…To date, fish tlr4 genes have only been identified in cyprinids (Meijer et al, 2004;Jault et al, 2004;Su et al, 2009). After searching the Zebrafish Information Network (ZFIN), we surprisingly found that zebrafish tlr4 exists in the form of a cluster containing three tlr4 tandem repeat genes which are closely arranged in the same direction within the chromosome (Supplementary data, Fig.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation and analysis of a novel grass carp toll-like receptor 4 (tlr4) gene cluster involved in the response to grass carp reovirus

Huang

Dong

Jang

et al. 2012

Developmental & Comparative Immunology

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation and analysis of a novel grass carp toll-like receptor 4 (tlr4) gene cluster involved in the response to grass carp reovirus

Huang

Dong

Jang

et al. 2012

Developmental & Comparative Immunology

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“…In fish, zebrafish genome contains at least 17 expressed TLR genes [12]. A prediction of the TLR family in the genome of the pufferfish Fugu rubripes was reported, indicating that this teleost contains counterparts of most human TLRs, with the exception of the LPS-specific TLR4 gene [13].…”

Section: Introductionmentioning

confidence: 99%

Genomic organization and expression analysis of Toll-like receptor 3 in grass carp (Ctenopharyngodon idella)

Jang

Yang

et al. 2009

Fish & Shellfish Immunology

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“…In fish, the zebrafish genome contains at least 17 expressed TLR genes (Meijer et al 2004). A prediction of the TLR family in the genome of the pufferfish Fugu rubripes was reported, indicating that this teleost contains counterparts of most human TLRs, with the exception of the LPS-specific TLR4 gene (Oshiumi et al 2003a).…”

Section: Introductionmentioning

confidence: 99%

Toll-like receptor 3 regulates Mx expression in rare minnow Gobiocypris rarus after viral infection

Zhang

Wang

et al. 2008

Immunogenetics

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Toll-like receptor 3 (TLR3) plays a key role in activating immune responses during viral infection. To study the genes involved in the regulatory function of TLR3 in the rare minnow Gobiocypris rarus after viral infection, a full-length cDNA of TLR3 (GrTLR3) with a splice variant (GrTLR3s) was identified by homologous cloning and RACE techniques. The antiviral effector molecule Mx gene was cloned and partially sequenced. The mRNA expression levels of GrTLR3, GrTLR3s, and Mx were studied in different tissues before and after virus infection by real-time quantitative RT-PCR. The transcripts of all three genes in liver were significantly increased following GCRV infection (P<0.05). The mRNA levels in liver were upregulated at 24 h post-injection for GrTLR3 and GrTLR3s, and at 12 h for Mx. The upregulated expression levels were several folds for GrTLR3s, tens of folds for GrTLR3, and hundreds of folds for Mx. By semiquantitative RT-PCR, GrTLR3 and Mx expressed at all the developmental stages, whereas GrTLR3s could only be detected at later developmental stages. Using RNAi and transgenic techniques, GrTLR3 mediated Mx expression but GrTLR3s did not. The time-dependent upregulation of receptor and effector, and the Mx over-expression dependent on TLR3, indicated that GrTLR3 regulated Mx expression in viral infection through a configuration change in rare minnow, and its splice variant did not contribute to the process.

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Expression analysis of the Toll-like receptor and TIR domain adaptor families of zebrafish

Cited by 482 publications

References 44 publications

Isolation and analysis of a novel grass carp toll-like receptor 4 (tlr4) gene cluster involved in the response to grass carp reovirus

Isolation and analysis of a novel grass carp toll-like receptor 4 (tlr4) gene cluster involved in the response to grass carp reovirus

Genomic organization and expression analysis of Toll-like receptor 3 in grass carp (Ctenopharyngodon idella)

Toll-like receptor 3 regulates Mx expression in rare minnow Gobiocypris rarus after viral infection

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