Expression Analysis of Six Paralogous Human Hyaluronidase Genes Clustered on Chromosomes 3p21 and 7q31

Csóka, Antonei B.; Scherer, Stephen W.; Stern, Robert

doi:10.1006/geno.1999.5876

Cited by 232 publications

(227 citation statements)

References 26 publications

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“…These data suggest that Hyal5 is expressed exclusively in the testis, and the expression may be specific for pachytene spermatocytes and round spermatids. It should be noted that no DNA fragment corresponding to Hyal4 was amplified by RT-PCR (data not shown), consistent with the fact that Hyal4 is expressed specifically in the placenta and skeletal muscle (5).…”

Section: Resultssupporting

confidence: 75%

Identification of a hyaluronidase, Hyal5, involved in penetration of mouse sperm through cumulus mass

Kim

Baba

Kimura

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

121

171

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A glycosylphosphatidylinositol (GPI)-anchored hyaluronidase, PH-20, on the sperm surface has long been believed to assist sperm penetration through the cumulus mass surrounding the eggs. However, mouse sperm lacking PH-20 were still capable of penetrating the cumulus mass despite a delayed dispersal of cumulus cells. Intriguingly, a 55-kDa hyaluronan-hydrolyzing protein was abundantly present in wild-type and PH-20-deficient mouse sperm. In this study, we purified the 55-kDa mouse protein from soluble protein extracts released from epididymal sperm by acrosome reaction and identified as a hyaluronidase, Hyal5.

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Section: Resultssupporting

confidence: 75%

Identification of a hyaluronidase, Hyal5, involved in penetration of mouse sperm through cumulus mass

Kim

Baba

Kimura

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

121

171

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“…However, none of the human hyaluronidase family members besides HYAL2 mediated JSRV transduction (Table 1). HYAL2 is widely expressed in most tissues, with the notable exception of brain (7,17,18), and closely related orthologs of the HYAL2 gene are found in rodents. Thus, the resistance of rodent cells to JSRV vector transduction suggests that these cells express a nonfunctional receptor.…”

Section: Resultsmentioning

confidence: 99%

Candidate tumor suppressor HYAL2 is a glycosylphosphatidylinositol (GPI)-anchored cell-surface receptor for jaagsiekte sheep retrovirus, the envelope protein of which mediates oncogenic transformation

Sharath

Duh

Вигдорович

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

305

342

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Jaagsiekte sheep retrovirus (JSRV) can induce rapid, multifocal lung cancer, but JSRV is a simple retrovirus having no known oncogenes. Here we show that the envelope (env) gene of JSRV has the unusual property that it can induce transformation in rat fibroblasts, and thus is likely to be responsible for oncogenesis in animals. Retrovirus entry into cells is mediated by Env interaction with particular cell-surface receptors, and we have used phenotypic screening of radiation hybrid cell lines to identify the candidate lung cancer tumor suppressor HYAL2͞LUCA2 as the receptor for JSRV. HYAL2 was previously described as a lysosomal hyaluronidase, but we show that HYAL2 is actually a glycosylphosphatidylinositol (GPI)-anchored cell-surface protein. Furthermore, we could not detect hyaluronidase activity associated with or secreted by cells expressing HYAL2, whereas we could easily detect such activity from cells expressing the related serum hyaluronidase HYAL1. Although the function of HYAL2 is currently unknown, other GPI-anchored proteins are involved in signal transduction, and some mediate mitogenic responses, suggesting a potential role of HYAL2 in JSRV Env-mediated oncogenesis. Lung cancer induced by JSRV closely resembles human bronchiolo-alveolar carcinoma, a disease that is increasing in frequency and now accounts for Ϸ25% of all lung cancer. The finding that JSRV env is oncogenic and the identification of HYAL2 as the JSRV receptor provide tools for further investigation of the mechanism of JSRV oncogenesis and its relationship to human bronchiolo-alveolar carcinoma.J aagsiekte sheep retrovirus (JSRV) is the causative agent of a contagious lung cancer of sheep called ovine pulmonary carcinoma or sheep pulmonary adenomatosis (1). Tumors originate from type II secretory alveolar and nonciliated bronchiolar epithelial cells, and late stages of the disease are accompanied by the secretion of copious lung fluid containing the virus. Purified virus induces multifocal tumors in as little as 10 days (2), suggesting the role of a viral oncogene. However, JSRV is a simple retrovirus with typical gag, pol, and env genes and no known oncogenes. The viral structural (Gag) and enzymatic (Pol) proteins are unlikely to be responsible, because they interact primarily with viral components, but there is precedent for alteration of cellular functions by the envelope (Env) protein of retroviruses, which interacts with cellular components to mediate virus entry. For example, the deleted Env protein of the Friend spleen focus-forming virus causes erythroleukemia by binding to and activating the erythropoietin receptor (3). If the JSRV Env protein was indeed oncogenic, identification of the host cell receptor for JSRV would provide key insights into the oncogenic mechanism of this highly pathogenic retrovirus. Furthermore, the contagious nature of JSRV and its ability to survive exposure to proteases and surfactants present in lung fluid suggest that vectors based on JSRV might be useful for gene therapy targeted to the lung, pr...

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“…HYAL2 (hyaluronidase 2, also known as LUCA2) is expressed at high levels in most tissues (including heart, placenta, lung, liver, skeletal muscle and kidney) but absent or poorly expressed in brain (Lepperdinger et al, 1998;Csoka et al, 1999;Lerman and Minna, 2000). Loss of expression in adult brain is reported to be due to promoter methylation (Lepperdinger et al, 2001).…”

Section: Hyal2/luca2mentioning

confidence: 99%

Evaluation of the 3p21.3 tumour-suppressor gene cluster

2007

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Expression Analysis of Six Paralogous Human Hyaluronidase Genes Clustered on Chromosomes 3p21 and 7q31

Cited by 232 publications

References 26 publications

Identification of a hyaluronidase, Hyal5, involved in penetration of mouse sperm through cumulus mass

Identification of a hyaluronidase, Hyal5, involved in penetration of mouse sperm through cumulus mass

Candidate tumor suppressor HYAL2 is a glycosylphosphatidylinositol (GPI)-anchored cell-surface receptor for jaagsiekte sheep retrovirus, the envelope protein of which mediates oncogenic transformation

Evaluation of the 3p21.3 tumour-suppressor gene cluster

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