2017
DOI: 10.1080/09168451.2017.1336921
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Expression analysis of polyphenol oxidase isozymes by active staining method and tissue browning of head lettuce (Lactuca sativa L.)

Abstract: Browning of plant tissue is generally considered attributable to enzymatic oxidation by polyphenol oxidase (PPO). Electrophoresis followed by activity staining has been used as an effective procedure to visually detect and isolate isozymes; however, it has not been applied for examination of various PPO isozymes in lettuce. Our study demonstrated that different lettuce PPO isozymes could be detected at different pH in active staining, and multiple isozymes were detected only under alkaline conditions. As a res… Show more

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Cited by 5 publications
(2 citation statements)
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“…The increase in the total polyphenol contents of young barley leaves, identified by the assays, resulted from the activation of phenylalanine ammonia lyase (PAL) ( Figure 4 A). This enzyme is involved in the biosynthesis of polyphenol precursors, mainly cinnamic acid [ 39 ]. A high level of polyphenols maintained in the plant material promotes a reduction in the activity of polyphenol oxidase (PPO) ( Figure 4 B).…”
Section: Resultsmentioning
confidence: 99%
“…The increase in the total polyphenol contents of young barley leaves, identified by the assays, resulted from the activation of phenylalanine ammonia lyase (PAL) ( Figure 4 A). This enzyme is involved in the biosynthesis of polyphenol precursors, mainly cinnamic acid [ 39 ]. A high level of polyphenols maintained in the plant material promotes a reduction in the activity of polyphenol oxidase (PPO) ( Figure 4 B).…”
Section: Resultsmentioning
confidence: 99%
“…In order to measure the performance of the filtering algorithm, the signal-to-noise ratio (SNR) [ 24 , 25 ] is introduced to evaluate the filtering effect of the algorithm. The lower the signal-to-noise ratio, the worse the signal quality; the higher the signal-to-noise ratio, the better the signal quality [ 26 ].…”
Section: Methodsmentioning
confidence: 99%