Expression Analysis of PKS13, FG08079.1 and PKS10 Genes in Fusarium graminearum and Fusarium culmorum

Yörük, Emre; Karlık, Elif; Gazdağlı, Aylin; Kayis, Müyesser; Kaya, Funda; Albayrak, Gülruh

doi:10.15171/ijb.1035

Cited by 2 publications

(1 citation statement)

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“…Therefore, the protein product of FG08079 is a cytochrome P450 and has low identity with P450, which is involved in trichothecene biosynthesis. 8 This is consistent with the results of Yörük et al, 30 showing that FG08079 transcripts were present in five F. graminearum, five F. culmorum, and 10 Fusarium isolates that produced butenolide under culture conditions.…”

Section: Discussionsupporting

confidence: 90%

Effect of Hibiscus Sabdariffa Extract on Growth of Fusarium graminearum and the Expression of TRI4 and FG08079 Genes of the Fungus

Narouei

Pirnia

Sarani

et al. 2021

Int J Basic Sci Med

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Introduction: Fusarium graminearum produces trichothecenes, such as deoxynivalenol and secondary metabolite butenolide, which cause profound health problems in humans. In this research, the effect of acetone extract of Hibiscus sabdariffa is evaluated on growth of F. graminearum and expression of TRI4 and FG08079 genes, which are involved in deoxynivalenol and butenolide biosynthetic pathways, respectively. Methods: Minimal inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were determined. The expression of TRI4 and FG08079 genes were evaluated by teal-time PCR technique.Results: The MIC and MFC for acetone extract of Hibiscus sabdariffa against F. graminearum were 200 mg/mL and 400 mg/mL, respectively. Expression of TRI4 and FG08079 genes were significantly decreased by the acetone extract of red tea. Conclusion: The results showed that acetone extract of Hibiscus sabdariffa has inhibitory and fungicidal effects on F. graminearum and is effective in reducing the expression of TRI4 and FG08079 genes, which play important roles in deoxynivalenol and butenolide production.

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Section: Discussionsupporting

confidence: 90%