Expression analysis of opaque2, crtRB1 and shrunken2 genes during different stages of kernel development in biofortified sweet corn

Baveja, Aanchal; Chhabra, Rashmi; Panda, Kusuma Kumari; Muthusamy, Vignesh; Mehta, Brijesh K.; Mishra, Subhra J.; Zunjare, Rajkumar U.; Hossain, Firoz

doi:10.1016/j.jcs.2022.103466

Cited by 6 publications

(4 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The highest mean expression of o2 gene was observed at 15 DAP, while the lowest mean expression was observed at 45 DAP. The decreasing pattern of amino acids (lysine and tryptophan) with kernel maturity indicates that delay would lead to the loss of nutritional quality [32,34]. As moisture content in maize kernels decreases, genes involved in the production of micronutrients are prevented from being transcribed [35].…”

Section: Expression Pattern Of O2 and Its Effect On Lysine And Trypto...mentioning

confidence: 99%

Expression Dynamics of lpa1 Gene and Accumulation Pattern of Phytate in Maize Genotypes Possessing opaque2 and crtRB1 Genes at Different Stages of Kernel Development

Bhatt

Muthusamy

Panda

et al. 2023

Plants

Self Cite

View full text Add to dashboard Cite

Phytic acid (PA) acts as a storehouse for the majority of the mineral phosphorous (P) in maize; ~80% of the total P stored as phytate P is not available to monogastric animals and thereby causes eutrophication. In addition, phytic acid chelates positively charged minerals making them unavailable in the diet. The mutant lpa1-1 allele reduces PA more than the wild-type LPA1 allele. Further, mutant gene opaque2 (o2) enhances lysine and tryptophan and crtRB1 enhances provitamin-A (proA) more than wild-type O2 and CRTRB1 alleles, respectively. So far, the expression pattern of the mutant lpa1-1 allele has not been analysed in maize genotypes rich in lysine, tryptophan and proA. Here, we analysed the expression pattern of wild and mutant alleles of LPA1, O2 and CRTRB1 genes in inbreds with (i) mutant lpa1-1, o2 and crtRB1 alleles, (ii) wild-type LPA1 allele and mutant o2 and crtRB1 alleles and (iii) wild-type LPA1, O2 and CRTRB1 alleles at 15, 30 and 45 days after pollination (DAP). The average reduction of PA/total phosphorous (TP) in lpa1-1 mutant inbreds was 29.30% over wild-type LPA1 allele. The o2 and crtRB1-based inbreds possessed ~two-fold higher amounts of lysine and tryptophan, and four-fold higher amounts of proA compared to wild-type alleles. The transcript levels of lpa1-1, o2 and crtRB1 genes in lpa1-1-based inbreds were significantly lower than their wild-type versions across kernel development. The lpa1-1, o2 and crtRB1 genes reached their highest peak at 15 DAP. The correlation of transcript levels of lpa1-1 was positive for PA/TP (r = 0.980), whereas it was negative with inorganic phosphorous (iP) (r = −0.950). The o2 and crtRB1 transcripts showed negative correlations with lysine (r = −0.887) and tryptophan (r = −0.893), and proA (r = −0.940), respectively. This is the first comprehensive study on lpa1-1 expression in the maize inbreds during different kernel development stages. The information generated here offers great potential for comprehending the dynamics of phytic acid regulation in maize.

show abstract

Section: Expression Pattern Of O2 and Its Effect On Lysine And Trypto...mentioning

confidence: 99%

Expression Dynamics of lpa1 Gene and Accumulation Pattern of Phytate in Maize Genotypes Possessing opaque2 and crtRB1 Genes at Different Stages of Kernel Development

Bhatt

Muthusamy

Panda

et al. 2023

Plants

Self Cite

View full text Add to dashboard Cite

show abstract

“…As a result, the introgressed lines retained their sweetness and were on par with the recurrent parent. The Sh2 gene is located in chromosome 3 and is responsible for coding a large subunit of ADPglucose pyrophosphorylase (AGPase), which synthesizes ADP-glucose from glucose-1-phosphate and adenosine triphosphate (Baveja et al, 2022). This conversion is blocked by the recessive sh2 allele, which restricts the starch biosynthesis of amylose and amylopectin.…”

Section: Kernel Sweetness and Nutritional Qualitymentioning

confidence: 99%

The resurrection of sweet corn inbred SC11-2 using marker aided breeding for β-carotene

Saha

Rathinavel

Manoharan

et al. 2022

Front. Sustain. Food Syst.

View full text Add to dashboard Cite

Sweet corn has dominated the urban market due to its sweetness, tenderness, and ease of digestibility. It's import and export values have dramatically increased during the past 10 years as a fresh, processed, and preserved commodity. However, the commercially available sweet corns are deficient in β-carotene. In our study, we introgressed the favorable allele of crtRB1 (responsible for high β-carotene) into the recurrent sweet corn inbred SC11-2 from maize donor parent UMI1230β1+ to develop the β-carotene-rich sweet corn genotype by marker aided breeding. The crtRB1 3′TE InDel marker was utilized for foreground selection of favorable genotype. A total of 103 polymorphic SSR markers were employed for background selection, resulting in a 96% recovery of recurrent parent genome (RPG). We recorded high β-carotene content (9.878–10.645 μg/g) in the introgressed lines compared to the recurrent parent, SC11-2 (0.989 μg/g). The sugar content ranged from 18 to 19.10% and was on par with the recurrent parent (20.40%). These biofortified inbreds can be used as a donor in maize breeding programs to develop sweet corn genotypes with high β-carotene content.

show abstract

“…Recently, sweet corn hybrids were biofortified with three nutritional genes ( sh2 , lcyE , crtRB1 , and o2 ) through the MABB approach with the help of trait-linked molecular markers. By using the MABB approach and sh2 , o2 , lcyE , and crtRB1 genes, Baveja et al created multinutrient-rich biofortified sweet corn hybrids . Recently, Chauhan et al pyramided the vte4 and crtRB1 genes, which are responsible for vitamin E and β-carotene …”

Section: Introductionmentioning

confidence: 99%

“…By using the MABB approach and sh2, o2, lcyE, and crtRB1 genes, Baveja et al created multinutrient-rich biofortified sweet corn hybrids. 16 Recently, Chauhan et al pyramided the vte4 and crtRB1 genes, which are responsible for vitamin E and β-carotene. 17 USC1-2-3-1, SC1107, and 12039-1 are popular inbreds that possess sweetness with desirable agronomic performance and are also parents for popular sweet corn hybrids.…”

Section: ■ Introductionmentioning

confidence: 99%

Marker-Assisted Genetic Enhancement of Provitamin A in Parental Lines of Sweet Corn Hybrids

Rathinavel,

Chandran,

Chellamuthu

et al. 2023

ACS Agric. Sci. Technol.

View full text Add to dashboard Cite

Sweet corn is cultivated worldwide in tropical and temperate regions, and it is consumed favorably due to its sweet taste, but it is poor in provitamin A carotenoids. For these reasons, by adopting a marker-assisted backcross breeding (MABB) approach, we aimed to enhance the β-carotene concentration in sweet corn inbreds (USC1-2-3-1, SC1107, and 12039-1), which are the parents of popular sweet corn hybrids. β-carotene-rich inbred lines UMI1230β + crossed with these inbreds and progenies are selected based on the gene-specific markers (foreground selection) and SSR markers (background selection). As a result, four improved lines from each cross, viz., USC1-2-3-1 × UMI1230β + , SC1107 × UMI1230β + , and 12039-1 × UMI1230β + with high βcarotene concentration and good agronomic performance (>80%) were obtained. These lines were used to produce hybrids with improved vitamin A content. Furthermore, the improved lines were used to develop the hybrids and tested along with the original hybrids. The hybrids produced by crossing improved lines were on par with the original hybrids regarding grain yield and sweetness with an added advantage of β-carotene. These improved β-carotene-rich sweet corn inbreds and hybrids have enormous potential to reduce malnutrition in a sustainable and economical way.

show abstract

Expression analysis of opaque2, crtRB1 and shrunken2 genes during different stages of kernel development in biofortified sweet corn

Cited by 6 publications

References 31 publications

Expression Dynamics of lpa1 Gene and Accumulation Pattern of Phytate in Maize Genotypes Possessing opaque2 and crtRB1 Genes at Different Stages of Kernel Development

Expression Dynamics of lpa1 Gene and Accumulation Pattern of Phytate in Maize Genotypes Possessing opaque2 and crtRB1 Genes at Different Stages of Kernel Development

The resurrection of sweet corn inbred SC11-2 using marker aided breeding for β-carotene

Marker-Assisted Genetic Enhancement of Provitamin A in Parental Lines of Sweet Corn Hybrids

Contact Info

Product

Resources

About