Expression Analysis of MaTGA8 Transcription Factor in Banana and Its Defence Functional Analysis by Overexpression in Arabidopsis

Abstract

TGA transcription factor is a member of the D subfamily of the basic region-leucine zippers (bZIP) family. It is a type of transcription factor that was first identified in plants and is the main regulator in plant development and physiological processes, including morphogenesis and seed formation in response to abiotic and biotic stress and maintaining plant growth. The present study examined the sequence of the MaTGA8 transcription factor, the sequence of which belonged to subfamily D of the bZIP and had multiple cis-acting elements such as the G-box, TCA-element, TGACG-element, and P-box. Quantitative real time polymerase chain reaction (qRT-PCR) analyses showed that MaTGA8 was significantly down-regulated by the soil-borne fungus Fusarium oxysporum f. sp. cubense race 4 (Foc TR4). Under the induction of salicylic acid (SA), MaTGA8 was down-regulated, while different members of the MaNPR1 family responded significantly differently. Among them, MaNPR11 and MaNPR3 showed an overall upward trend, and the expression level of MaNPR4, MaNPR8, and MaNPR13 was higher than other members. MaTGA8 is a nuclear-localized transcription factor through strong interaction with MaNPR11 or weaker interaction with MaNPR4, and it is implied that the MaPR gene can be activated. In addition, the MaTGA8 transgenic Arabidopsis has obvious disease resistance and higher chlorophyll content than the wild-type Arabidopsis with the infection of Foc TR4. These results indicate that MaTGA8 may enhance the resistance of bananas to Foc TR4 by interacting with MaNPR11 or MaNPR4. This study provides a basis for further research on the application of banana TGA transcription factors in Foc TR4 stress and disease resistance and molecular breeding programs.

Metabolic resistance to acetolactate synthase inhibitors in Beckmannia syzigachne: identification of CYP81Q32 and its transcription regulation

“…Effect of SA on BsTGAL6/BsCYP81Q expression and B. syzigachne resistance to mesosulfuron-methyl Because exogenous application with SA can modulate the expression of TGA genes (Lin et al, 2021;Moon et al, 2018), SA was used to examine the role of TGAL6 on BsCYP81Q expression and consequently on B. syzigachne resistance to mesosulfuronmethyl. SA was applied to the two-to three-leaf stage R and S B. syzigachne plants and expression of TGAL6 and BsCYP81Q was RT-qPCR measured at 0, 6, 12, and 24 h after treatment.…”

The Grapevine Transcription Factor VvTGA8 Enhances Resistance to White Rot via the Salicylic Acid Signaling Pathway in Tomato

“…VvTGA8 overexpression in S. lycopersicum significantly improved the resistance to C. vitis and activated the expression of SlPR1 and SlPR2, further supporting the role of VvTGA8 in resistance to C. vitis (Figure 7). A study has shown that the overexpression of MaTGA8 from banana significantly enhances resistance to Fusarium oxysporum TR4 in A. thaliana [38]. In addition, TGA8 promotes root growth by promoting stem cell regeneration, meristem activity, and cell elongation in A. thaliana [27].…”

Combined analysis of mRNA and miRNA reveals the banana potassium absorption regulatory network and validation of miRNA160a

“…Referred to the method of Lin et al(2020), pCAMBIA-1300 was selected as the vector and EcorV was selected as the restriction site [17]. The recombinant expression vector pBWA(V)HS-miR160a was transformed into Agrobacterium tumefaciens strain GV3101 (Wuhan Biorun Biotechnology Co., Ltd., Wuhan, China) and then was transformed into Arabidopsis thaliana (Col-0) by in orescence infection.…”