Expression analysis of imbalanced genes in prostate carcinoma using tissue microarrays

Prowatke, I.; Devens, Frauke; Benner, Axel; Gröne, Elisabeth F.; Mertens, Daniel; Gröne, Hermann–Josef; Lichter, Peter; Joos, Stefan

doi:10.1038/sj.bjc.6603490

Cited by 79 publications

(59 citation statements)

References 30 publications

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“…The correlation between reduced expression of the NM23-H1 gene and a higher metastatic potential and worse prognosis has been shown in carcinomas of the breast, 20,21 liver, 22 lung 23 and prostate. 24 On the contrary, the opposite trend has been reported in thyroid carcinomas, 25 neuroblastoma, 26 and head and neck carcinomas. 27 Obviously, the NM23-H1 protein has a different and possibly variable role in tumors of different origins.…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle delivery of anti-metastatic NM23-H1 gene improves chemotherapy in a mouse tumor model

Xiang

Zhang

et al. 2008

Cancer Gene Ther

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Gene therapy provides a promising approach for cancer treatment. Earlier studies suggested that poly-L-lysine-modified iron oxide nanoparticles (IONP-PLL) might be a promising gene delivery system that can transfect DNA efficiently in vitro and in vivo. In this study we used IONP-PLL as gene carriers to deliver the NM23-H1 gene, the first suppressor gene of cancer metastasis, to tumor cells in vivo. The intravenous injection of IONP-PLL carrying NM23-H1-GFP plasmid DNA significantly extended the survival time of an experimental pulmonary metastasis mouse model. In the IONP-PLL/NM23-H1-GFP-treated group, metastasis was clearly suppressed compared with the group treated with free NM23-H1-GFP plasmid. Furthermore, this gene therapy combined with cyclophosphamide treatment resulted in longer survival times and greater suppression of metastasis growth. In conclusion, treatment with IONP-PLL nanoparticles incorporating the NM23-H1gene is an efficient gene therapy method, and it is even more effective in combination with chemotherapy. This approach appears to be a promising strategy for treatment of metastatic tumors.

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Section: Introductionmentioning

confidence: 99%

Nanoparticle delivery of anti-metastatic NM23-H1 gene improves chemotherapy in a mouse tumor model

Xiang

Zhang

et al. 2008

Cancer Gene Ther

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“…There is also evidence that expression of βARK1, which desensitizes ADRB2, is lost in prostate tumors with high Gleason scores. This may lead to prolonged activation of adrenaline/ADRB2/PKA/BAD signaling (52).…”

Section: Figurementioning

confidence: 99%

Behavioral stress accelerates prostate cancer development in mice

Hassan

Karpova²,

Daniele³

et al. 2013

J. Clin. Invest.

170

211

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Prostate cancer patients have increased levels of stress and anxiety. Conversely, men who take beta blockers, which interfere with signaling from the stress hormones adrenaline and noradrenaline, have a lower incidence of prostate cancer; however, the mechanisms underlying stress-prostate cancer interactions are unknown. Here, we report that stress promotes prostate carcinogenesis in mice in an adrenaline-dependent manner. Behavioral stress inhibited apoptosis and delayed prostate tumor involution both in phosphatase and tensin homolog-deficient (PTEN-deficient) prostate cancer xenografts treated with PI3K inhibitor and in prostate tumors of mice with prostate-restricted expression of c-MYC (Hi-Myc mice) subjected to androgen ablation therapy with bicalutamide. Additionally, stress accelerated prostate cancer development in Hi-Myc mice. The effects of stress were prevented by treatment with the selective β 2 -adrenergic receptor (ADRB2) antagonist ICI118,551 or by inducible expression of PKA inhibitor (PKI) or of BCL2-associated death promoter (BAD) with a mutated PKA phosphorylation site (BAD S112A ) in xenograft tumors. Effects of stress were also blocked in Hi-Myc mice expressing phosphorylation-deficient BAD (BAD 3SA ). These results demonstrate interactions between prostate tumors and the psychosocial environment mediated by activation of an adrenaline/ADRB2/ PKA/BAD antiapoptotic signaling pathway. Our findings could be used to identify prostate cancer patients who could benefit from stress reduction or from pharmacological inhibition of stress-induced signaling.

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“…Intricate interplay of dephosphorylation and kinase activity governs the normal process of cell cycle. Genetic alterations in the catalytic and regulatory subunits of these protein phosphatases have been reported in various human cancers including oral squamous cell carcinoma and prostate cancer (41,42). In an extensive study of genetic alterations compiled using oligonucleotide-based array, comparative genomic hybridization showed that 11q13.4 is differentially deleted specifically in primary glioma as opposed to secondary GBM and has been classified as high-priority primary GBM subtype-unique minimal common regions.…”

Section: Discussionmentioning

confidence: 99%

Glioblastoma-Specific Protein Interaction Network Identifies PP1A and CSK21 as Connecting Molecules between Cell Cycle–Associated Genes

Ladha

Donakonda²,

Agrawal³

et al. 2010

Cancer Research

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Glioblastoma (GBM; grade IV astrocytoma) is a very aggressive form of brain cancer with a poor survival and few qualified predictive markers. This study integrates experimentally validated genes that showed specific upregulation in GBM along with their protein-protein interaction information. A system level analysis was used to construct GBM-specific network. Computation of topological parameters of networks showed scale-free pattern and hierarchical organization. From the large network involving 1,447 proteins, we synthesized subnetworks and annotated them with highly enriched biological processes. A careful dissection of the functional modules, important nodes, and their connections identified two novel intermediary molecules CSK21 and protein phosphatase 1 α (PP1A) connecting the two subnetworks CDC2-PTEN-TOP2A-CAV1-P53 and CDC2-CAV1-RB-P53-PTEN, respectively. Real-time quantitative reverse transcription-PCR analysis revealed CSK21 to be moderately upregulated and PP1A to be overexpressed by 20-fold in GBM tumor samples. Immunohistochemical staining revealed nuclear expression of PP1A only in GBM samples. Thus, CSK21 and PP1A, whose functions are intimately associated with cell cycle regulation, might play key role in gliomagenesis.

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Expression analysis of imbalanced genes in prostate carcinoma using tissue microarrays

Cited by 79 publications

References 30 publications

Nanoparticle delivery of anti-metastatic NM23-H1 gene improves chemotherapy in a mouse tumor model

Nanoparticle delivery of anti-metastatic NM23-H1 gene improves chemotherapy in a mouse tumor model

Behavioral stress accelerates prostate cancer development in mice

Glioblastoma-Specific Protein Interaction Network Identifies PP1A and CSK21 as Connecting Molecules between Cell Cycle–Associated Genes

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