Expression Analysis of Genes for Callose Synthases and Rho-Type Small GTP-Binding Proteins That Are Related to Callose Synthesis in Rice Anther

Yamaguchi, Tomoya; Hayashi, Takami; Nakayama, Katsuhiro; Koike, Setsuo

doi:10.1271/bbb.70.639

Cited by 30 publications

(28 citation statements)

References 23 publications

(25 reference statements)

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“…Aniline blue staining showed the irregular stacking of callose on the mutant meiocytes (Figure S2). We monitored the expression levels of OsGSL5 (putative glucan synthase gene, AP003454 ), which encodes an anther‐specific callose synthase (Yamaguchi et al. , 2006), and Osg1 (glucanase, AB070742 ), which is required for callose degradation (Wan et al.…”

Section: Resultsmentioning

confidence: 99%

The rice geneDEFECTIVE TAPETUM AND MEIOCYTES 1(DTM1) is required for early tapetum development and meiosis

Kim

Lee

et al. 2012

The Plant Journal

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SUMMARYTapetum development and meiosis play crucial roles in anther development. Here we identified a rice gene, DEFECTIVE TAPETUM AND MEIOCYTES 1 (DTM1), which controls the early stages of that development. This gene encodes for an endoplasmic reticulum (ER) membrane protein that is present only in cereals. Our T-DNA insertion mutations gave rise to abnormal tapetal formation. Cellular organelles, especially the ER, were underdeveloped, which led to hampered differentiation and degeneration of the tapetum. In addition, the development of pollen mother cells was arrested at the early stages of meiotic prophase I. RNA in-situ hybridization analyses showed that DTM1 transcripts were most abundant in tapetal cells at stages 6 and 7, and moderately in the pollen mother cells and meiocytes. Transcripts of UDT1, which functions in tapetum development during early meiosis, were reduced in dtm1 anthers, as were those of PAIR1, which is involved in chromosome pairing and synapsis during meiosis. However, expression of MSP1 and MEL1, which function in anther wall specification and germ cell division, respectively, was not altered in the dtm1 mutant. Moreover, transcripts of DTM1 were reduced in msp1 mutant anthers, but not in udt1 and pair1 mutants. These results, together with their mutant phenotypes, suggest that DTM1 plays important roles in the ER membrane during early tapetum development, functioning after MSP1 and before UDT1, and also in meiocyte development, after MEL1 and before PAIR1.

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Section: Resultsmentioning

confidence: 99%

The rice geneDEFECTIVE TAPETUM AND MEIOCYTES 1(DTM1) is required for early tapetum development and meiosis

Kim

Lee

et al. 2012

The Plant Journal

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“…It is located between the cellulose cell wall and plasma membrane, playing a key role in the process of fertile pollen formation (Worrall et al 1992). Abnormal regulation of callose synthesis and dissolution may affect proper pollen wall formation (Chen et al 2007;Dong et al 2005;Worrall et al 1992;Yamaguchi et al 2006). In the present research, four microspores without a callose wall were observed held together in a tetrad-like structure in the anthers of the male sterile plant from 01GAB.…”

Section: Discussionmentioning

confidence: 99%

Genetic and cytological analysis of a new spontaneous male sterility in radish (Raphanus sativus L.)

et al. 2011

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A male sterile plant appeared in the radish breeding program at the Hubei Academy of Agricultural Sciences, Hubei, China. In its progeny, a twotype (half of plants male sterile, the other half male fertile) line 01GAB was established. An F 2 population of 260 plants from a cross of male-sterile 01GAB and a male fertile line 9802H segregated for male fertility in a 3:1 ratio indicating that fertility was restored by a single dominant gene, here designated RsMs. A PCRbased DNA marker specific to the male fertility Rfob gene in 9802H was absent in 01GAB. Linkage analysis placed the RsMs locus 10.7 cM away from the Rfo locus. In an F 2 population of hybrids between 01GAB and male fertile 9802B, a co-dominant DNA marker for the RSultr3.2A (a radish sulfate transporter gene) locus was linked to the RsMs locus at 1.5 cM suggesting that fertility restoration in 01GAB was located in the region with known male sterility restorers in radish. However, no maintainer for the 01GAB source of male sterility has been identified so far. Cytological observations have shown that the abnormalities in male sterile anthers first appeared in tapetum at the tetrad stage, followed by a hypertrophy of the tapetal cells at the vacuolate microspore period. These results suggest that male sterility in 01GAB is likely to be genetic in nature, or it may represent a new type of the cytoplasmic male sterility.

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“…Among the 10 GSLs in rice, the expression of OsGSL5 was dramatically downregulated by cool treatment, it indicated that cool conditions affected GSL activity (especially GSL5) during male reproductive development. 41 Phytosphingosine, an inhibitor of yeast GSLs in vivo, was shown to be located at the endoplasmic reticulum. 42 In Neurospora crassa and higher plants, the FKS domain of GSLs binds directly to UDP-glucose.…”

Section: Callose Biosynthesis During Male Reproductive Development Inmentioning

confidence: 99%

Callose synthesis during reproductive development in monocotyledonous and dicotyledonous plants

Xiao

Han

2015

Plant Signaling & Behavior

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Callose, a linear b-1,3-glucan molecule, plays important roles in a variety of processes in angiosperms, including development and the response to biotic and abiotic stress. Despite the importance of callose deposition, our understanding of the roles of callose in rice reproductive development and the regulation of callose biosynthesis is limited. GLUCAN SYNTHASE-LIKE genes encode callose synthases (GSLs), which function in the production of callose at diverse sites in plants. Studies have shown that callose participated in plant reproductive development, and that the timely deposition and degradation of callose were essential for normal male gametophyte development. In this mini-review, we described conserved sequences found in GSL family proteins from monocotyledonous (Oryza sativa and Zea mays) and dicotyledonous (Arabidopsis thaliana and Glycine max) plants. We also describe the latest findings on callose biosynthesis and deposition during reproductive development and discuss future challenges in unraveling the mechanism of callose synthesis and deposition in higher plants.

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Expression Analysis of Genes for Callose Synthases and Rho-Type Small GTP-Binding Proteins That Are Related to Callose Synthesis in Rice Anther

Cited by 30 publications

References 23 publications

The rice geneDEFECTIVE TAPETUM AND MEIOCYTES 1(DTM1) is required for early tapetum development and meiosis

The rice geneDEFECTIVE TAPETUM AND MEIOCYTES 1(DTM1) is required for early tapetum development and meiosis

Genetic and cytological analysis of a new spontaneous male sterility in radish (Raphanus sativus L.)

Callose synthesis during reproductive development in monocotyledonous and dicotyledonous plants

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