Expression analysis of genes encoding plasma membrane aquaporins during seed and fruit development in tomato

Shiota, Hajime; Sudoh, Takehiro; Tanaka, Ichiro

doi:10.1016/j.plantsci.2006.03.021

Cited by 30 publications

(23 citation statements)

References 28 publications

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“…Therefore, the expression of aquaporin genes may be independently regulated in an organ-and stagespecific manner, while the amount of aquaporin proteins can be regulated at the translational level and by the rate of protein turnover [13,33,43,44]. Meanwhile, there are numerous reports providing clear evidence that the abundance of aquaporins can be regulated by developmental and environmental factors [6,13,32].…”

Section: Aquaporin Gene Expression and Diurnal Fluctuations In Plantsmentioning

confidence: 99%

Advances in functional regulation mechanisms of plant aquaporins: Their diversity, gene expression, localization, structure and roles in plant soil-water relations (Review)

Shao¹,

Chu

Shao

et al. 2008

Molecular Membrane Biology

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Aquaporins are important molecules that control the moisture level of cells and water flow in plants. Plant aquaporins are present in various tissues, and play roles in water transport, cell differentiation and cell enlargement involved in plant growth and water relations. The insights into aquaporins' diversity, structure, expression, post-translational modification, permeability properties, subcellular location, etc., from considerable studies, can lead to an understanding of basic features of the water transport mechanism and increased illumination into plant water relations. Recent important advances in determining the structure and activity of different aquaporins give further details on the mechanism of functional regulation. Therefore, the current paper mainly focuses on aquaporin structure-function relationships, in order to understand the function and regulation of aquaporins at the cellular level and in the whole plant subjected to various environmental conditions. As a result, the straightforward view is that most aquaporins in plants are to regulate water flow mainly at cellular scale, which is the most widespread general interpretation of the physiological and functional assays in plants.

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Section: Aquaporin Gene Expression and Diurnal Fluctuations In Plantsmentioning

confidence: 99%

Advances in functional regulation mechanisms of plant aquaporins: Their diversity, gene expression, localization, structure and roles in plant soil-water relations (Review)

Shao¹,

Chu

Shao

et al. 2008

Molecular Membrane Biology

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“…For instance, it was described that the inhibition of a membrane protein related to MIP family (LePIP1;5) increases organic acids and reduces sugar levels during tomato fruit ripening (Chen et al 2001). In apple, two putative plasma membrane intrinsic protein subtype 1 (PIP1) aquaporin genes were related to fruit development and osmotic stress by Hu et al (2003), and recently, Shiota et al (2006) reported that the expression of genes encoding PIPs in tomato changes during seed and fruit development. However, in all these cases, the authors have not performed functional characterization of the reported aquaporins.…”

Section: Introductionmentioning

confidence: 99%

A fruit‐specific plasma membrane aquaporin subtype PIP1;1 is regulated during strawberry (Fragaria × ananassa) fruit ripening

Mut

Bustamante

Martínez

et al. 2008

Physiologia Plantarum

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Despite the advances in the physiology of fruit ripening, the role and contribution of water pathways are still barely considered. Our aim was therefore to characterize aquaporins, proteins that render the molecular basis for putative regulatory mechanisms in water transport. We focused our work on strawberry (Fragaria xananassa) fruit, a non-climacteric fruit of special interest because of its forced brief commercial shelf life. A full-length cDNA was isolated with high homology with plasma membrane (PM) intrinsic proteins (named FaPIP1;1), showing a profile with high expression in fruit, less in ovaries and no detection at all in other parts. Its cellular localization was confirmed at the PM. As reported in other plasma membrane intrinsic proteins subtype 1 (PIP1s), when expressing the protein in Xenopus leavis oocytes, FaPIP1;1 shows low water permeability values that only increased when it is coexpressed with a plasma membrane intrinsic protein subtype 2. Northern blotting using total RNA shows that its expression increases during fruit ripening. Moreover, functional characterization of isolated PM vesicles from red stage fruit unequivocally demonstrates the presence of active water channels, i.e. high water permeability values and a low Arrhenius activation energy, both evidences of water transport mediated by proteins. Interestingly, as many ripening-related strawberry genes, the expression pattern of FaPIP1;1 was also repressed by the presence of auxins. We therefore report a fruit specific PIP1 aquaporin with an accumulation pattern tightly associated to auxins and to the ripening process that might be responsible for increasing water permeability at the level of the PM in ripe fruit.

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“…The regulation of R h by aquaporins is now well documented in roots (Martre et al, 2001;McElrone et al, 2007;Vandeleur et al, 2009), and oxidative gating of aquaporins has been reported to reduce hydraulic conductivity by 90% in cells of the giant algae Chara (Henzler et al, 2004). The results of previous work suggest that aquaporins play an important role in the regulation of water movement during the development of flowers, seeds, and fruits (Maurel et al, 1995;Gao et al, 1999;Picaud et al, 2003;Shiota et al, 2006;Zhou et al, 2007). Changes in the expression of the plasma membrane intrinsic protein (PIP) PIP1 and PIP2 aquaporin gene families have been noted in ripening grapes (Picaud et al, 2003;Fei et al, 2004), although the effects of these changes on water transport (membrane conductivity) have not been documented.…”

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confidence: 99%

Vascular Function in Grape Berries across Development and Its Relevance to Apparent Hydraulic Isolation

et al. 2009

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During the latter stages of development in fleshy fruit, water flow through the xylem declines markedly and the requirements of transpiration and further expansion are fulfilled primarily by the phloem. We evaluated the hypothesis that cessation of water transport through the xylem results from disruption or occlusion of pedicel and berry xylem conduits (hydraulic isolation). Xylem hydraulic resistance (R h ) was measured in developing fruit of grape (Vitis vinifera 'Chardonnay') 20 to 100 d after anthesis (DAA) and compared with observations of xylem anatomy by light and cryo-scanning electron microscopy and expression of six plasma membrane intrinsic protein (PIP) aquaporin genes (VvPIP1;1, VvPIP1;2, VvPIP1;3, VvPIP2;1, VvPIP2;2, VvPIP2;3). There was a significant increase in whole berry R h and receptacle R h in the latter stages of ripening (80-100 DAA), which was associated with deposition of gels or solutes in many receptacle xylem conduits. Peaks in the expression of some aquaporin isoforms corresponded to lower whole berry R h 60 to 80 DAA, and the increase in R h beginning at 80 DAA correlated with decreases in the expression of the two most predominantly expressed PIP genes. Although significant, the increase in berry R h was not great enough, and occurred too late in development, to explain the decline in xylem flow that occurs at 60 to 75 DAA. The evidence suggests that the fruit is not hydraulically isolated from the parent plant by xylem occlusion but, rather, is "hydraulically buffered" by water delivered via the phloem.

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Expression analysis of genes encoding plasma membrane aquaporins during seed and fruit development in tomato

Cited by 30 publications

References 28 publications

Advances in functional regulation mechanisms of plant aquaporins: Their diversity, gene expression, localization, structure and roles in plant soil-water relations (Review)

Advances in functional regulation mechanisms of plant aquaporins: Their diversity, gene expression, localization, structure and roles in plant soil-water relations (Review)

A fruit‐specific plasma membrane aquaporin subtype PIP1;1 is regulated during strawberry (Fragaria × ananassa) fruit ripening

Vascular Function in Grape Berries across Development and Its Relevance to Apparent Hydraulic Isolation

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