Expression analysis of dehydrin multigene family across tolerant and susceptible barley (Hordeum vulgare L.) genotypes in response to terminal drought stress

Karami, Amin; Shahbazi, Maryam; Niknam, Vahid; Shobbar, Zahra Sadat; Tafreshi, Razieh Sarabadani; Abedini, Raha; Mabood, H. E.

doi:10.1007/s11738-013-1266-1

Cited by 41 publications

(33 citation statements)

References 21 publications

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“…Based on the increase of their expression under different drought regimes, two dehydrins genes, HvDHN1 and HvDHN9, were chosen (Choi et al 1999;Suprunova et al 2004;Karami et al 2013). These genes showed increased expression under drought stress.…”

Section: Discussionmentioning

confidence: 99%

Physiological and Molecular Responses to Controlled Severe Drought in Two Barley (Hordeum VulgareL.) Genotypes

Harb

Samarah

2015

Journal of Crop Improvement

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Drought is a major abiotic stress that restricts growth and productivity of many crops. The objectives of this study were to assess the morphological and physiological responses of barley plants at the vegetative stage to controlled severe drought, and to test the expression of drought-responsive genes (HvHsdr4, HvNCED2, HvDHN1, HvDHN9, and HvCBF3) in a time course of drought treatment. Barley plants of two genotypes (Rum and Yarmouk) were exposed to controlled severe drought (25% field capacity) in a greenhouse experiment. The two genotypes showed a similar and significant reduction in net photosynthetic rate and plant height. At the molecular level, a differential gene expression of drought-responsive genes was shown between the two genotypes. The results indicate that drought-responsive genes can be useful marker genes to study the differential response of barley genotypes to drought stress conditions.

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Section: Discussionmentioning

confidence: 99%

Physiological and Molecular Responses to Controlled Severe Drought in Two Barley (Hordeum VulgareL.) Genotypes

Harb

Samarah

2015

Journal of Crop Improvement

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“…These three cultivars were treated with different concentrations (0,5,10,15,20,25, and 30%) of polyethylene glycol (PEG) 6000 (Sigma Aldrich, Saint Louis, MO, USA) at the three-leaf stage in an incubator at 25 o C with 2000 lx lighting intensity and a 14h:10h light:dark photoperiod. Sufficient PEG 6000 solution was added to each dish every day so that simulated drought conditions could be maintained for 3 d. Then the relative conductivity and malondialdehyde content of leaves were measured according to Karami et al (2013), while the soluble protein content of leaves was determined according to Bradford (1976).…”

Section: Polyethylene Glycol 6000 Treatmentmentioning

confidence: 99%

The response mechanism of the HVA1 Gene in hulless Barley under drought stress

Yao

et al. 2017

Ital J Agronomy

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HVA1, a member of LEA3 (late embryogenesis abundant protein, group 3), is closely related to water stress. However, the response of HVA1 to drought remains unknown in hulless barley. In this study, cultivars with high (Handizi), intermediate (Kunlun 12), and low (Dama) drought tolerance were selected from 28 hulless barley cultivars from the Tibet-Qinghai plateau to explore the drought response mechanism of HVA1. Then, HVA1 was cloned and the expression of the three cultivars was studied using exposure to polyethylene glycol (PEG) 6000. HVA1s in the three hulless barleys were highly homologous at the nucleotide and amino acid levels with over 99% identity. Real-time quantitative polymerase chain reaction showed that the expression level of HVA1 induced by PEG 6000 had a single peak curve in the three cultivars, but higher HVA1 transcript accumulation was seen in Handizi than in Kunlun 12 and Dama under the same drought stress. This result was also proved in eight hulless barleys. The expression level was a better predictor of drought resistance than the genetic structure of HVA1. IntroductionHulless barley (Hordeum vulgare L. var. nudum Hook. f.) is a selfing annual species, with naked grains when ripening. It is widely grown on the Qinghai-Tibet plateau (suffering serious drought stress) and has been a staple food for the Tibetan people since the fifth century CE (Liang et al., 2012). Drought is an important environmental constraint that limits the productivity of barley and other crops worldwide (Romanek et al., 2011). The growth and development of plants are restrained under the stress of drought with decreases in net photosynthetic rate, respiration, leaf osmoregulation ability, and cell membrane stability (Li et al., 2016). The most susceptible stages to drought are germination, early seedling growth, and grain filling. If plants can survive drought stress during these sensitive periods, the ability of the plant to survive additional drought exposure will increase (Liang et al., 2016).Plants have developed many physiological and biochemical reactions in response to adverse environmental conditions. Some compatible low-molecular-weight metabolites will be accumulated to protect cells against dehydration, and the most common of these is late embryogenesis abundant protein (LEA) (Park et al., 2003). LEA proteins are closely related to desiccation induced and regulated by abscisic acid (ABA) or dehydration signaling (Ramanjulu and Bartels, 2002). LEA proteins are involved in protection mechanisms against environmental stressors in plants (Liang et al., 2013). According to the homology of the amino acid sequence and presence of special primitive sequences, LEA proteins are categorised into six groups (Wise, 2003). The Hordeum vulgare aleurone1 (HVA1) gene, which belongs to group 3 LEA, is activated during cell dehydration caused by water deficit, salt stress, low temperature, or ABA induction (Romanek et al., 2011;Battaglia et al., 2008).Most researches on the HVA1 have focused on transformation. The H...

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“…Our finding is accordance with those of Kumar and Sharma, (2010) and Sharma et al (2016) observed reduction in relative water content when crop was imposed under drought environment. Karami et al 2013 find genotype whose maintain water status had more in antioxidant activity under drought condition. Behbahanizadeh, et al (2014) and Ram et al (2017) also reported that under drought stress conditions (moderate stress and severe stress) RWC, osmotic potential and water potential reduced in contrast to control condition and cultivars had a significant difference together in water relation traits.…”

Section: Relative Water Content (%)mentioning

confidence: 87%

Effect of Restricted Irrigation on Water Relation Traits and Antioxidant Activity

Kumar¹,

Ram²,

na³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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Expression analysis of dehydrin multigene family across tolerant and susceptible barley (Hordeum vulgare L.) genotypes in response to terminal drought stress

Cited by 41 publications

References 21 publications

Physiological and Molecular Responses to Controlled Severe Drought in Two Barley (Hordeum VulgareL.) Genotypes

Physiological and Molecular Responses to Controlled Severe Drought in Two Barley (Hordeum VulgareL.) Genotypes

The response mechanism of the HVA1 Gene in hulless Barley under drought stress

Effect of Restricted Irrigation on Water Relation Traits and Antioxidant Activity

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