Expression analysis of defense-related genes in wild kiwifruit (Actinidia rufa) tolerant to bacterial canker

Kaji, Ryoma; Yariuchi, Rina; Fujii, Yumi; Taniguchi, Shiduku; Uji, Yuya; Suzuki, Go; Kashihara, Keita; Kisaki, Gan; Suezawa, Katsuhiko; Ohtani, M.; Kataoka, Isao; Akimitsu, Kazuya; Gomi, Kenji

doi:10.1007/s10327-021-01024-7

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…Sinensis. 51,52 The nucleotide sequence of VcOMT contained 1107-bp and encoded a putative protein of 368 amino acids. The calculated isoelectric point was 4.99, and the predicted molecular mass of the amino acid sequence was 40.1 kDa.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Functional Analysis of a Methyltransferase Involved in Anthocyanin Biosynthesis from Blueberries (Vaccinium corymbosum)

Xie

Yang

Zhou

et al. 2022

J. Agric. Food Chem.

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Anthocyanins are natural water-soluble pigments that widely exist in plants, with various biological activities, including antioxidant, anti-obesity, and anti-diabetic activities. Currently, monomeric anthocyanins are mainly obtained through natural sources, which limits their availability. In the biosynthesis of anthocyanins, anthocyanin methyltransferases are recognized to play important roles in the water solubility and structural stability of anthocyanins. Blueberries are a rich source of anthocyanins with more than 30 chemical structures. However, the enzymes that were responsible for the methylation of anthocyanidin cores in blueberries had not been reported. Here, blueberries (Vaccinium corymbosum) have been selected as the candidate for characterization of the key enzyme. Phylogenic analysis, enzymatic activity assay, homology modeling, molecular simulation, protein expression and purification assay, site-directed mutation, isothermal titration calorimetry assay, and enzyme kinetic assay were used to identify the enzymatic function and molecular mechanism of VcOMT, which was responsible for the methylation of anthocyanidin cores. VcOMT could use delphinidin as a substrate but not cyanidin, petunidin, anthocyanins, flavonols, and flavonol glycosides. Ile191 and Glu198 were both identified as important amino acid residues for the binding interactions of anthocyanidins with VcOMT.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Functional Analysis of a Methyltransferase Involved in Anthocyanin Biosynthesis from Blueberries (Vaccinium corymbosum)

Xie

Yang

Zhou

et al. 2022

J. Agric. Food Chem.

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“…Impairments in several amino acids, including L-serine and aspartate, were also observed in Psa-infected plants, leading to extensive reprogramming of carbon (C) and N cycling in various metabolic pathways, including photosynthesis, C fixation, and the tricarboxylic acid cycle [127,140,164]. Several sugars, e.g., sucrose and fructose, were differently impaired in plant leaves, stems, or bleeding saps in later stages of Psa infection (20 days after infection) [127].…”

Section: Primary and Secondary Metabolismmentioning

confidence: 99%

“…In contrast, in the tolerant A. chinensis var. deliciosa Jinkui, PAL activity decreased, but lignin was constitutively higher or increased in several tolerant genotypes, including Jinkui, A. rufa, and A. arguta [75,127,143,164]. Considering these pieces of evidence, it has been hypothesized that plant responses against Psa are regulated by the allocation of phenolic substances into lignin biosynthesis through a mechanism involving the downregulation of MYB16 [143].…”

Section: Primary and Secondary Metabolismmentioning

confidence: 99%

Mitigation of Emergent Bacterial Pathogens Using Pseudomonas syringae pv. actinidiae as a Case Study—From Orchard to Gene and Everything in Between

Silva

Santos

Vasconcelos

et al. 2022

Crops

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Globalization propelled human migration and commercial exchanges at the global level, but woefully led to the introduction of non-indigenous organisms into several agroecological systems. These include pathogenic bacteria with devastating consequences for numerous crops of agronomical importance for food production worldwide. In the last decade, research efforts have focused on these noxious organisms, aiming to understand their evolutionary processes, degree of pathogenicity, and mitigation strategies, which have allowed stakeholders and policymakers to develop evidence-based regulatory norms to improve management practices and minimize production losses. One of these cases is the bacterium Pseudomonas syringae pv. actinidiae (Psa), the causal agent of the kiwifruit bacterial canker, which has been causing drastic production losses and added costs related to orchard management in the kiwifruit industry. Although Psa is presently considered a pandemic pathogen and far from being eradicated, the implementation of strict regulatory norms and the efforts employed by the scientific community allowed the mitigation, to some extent, of its negative impacts through an integrated pest management approach. This included implementing directive guidelines, modifying cultural practices, and searching for sources of plant resistance. However, bacterial pathogens often have high spatial and temporal variability, with new strains constantly arising through mutation, recombination, and gene flow, posing constant pressure to agroecosystems. This review aims to critically appraise the efforts developed to mitigate bacterial pathogens of agronomical impact, from orchard management to genome analysis, using Psa as a case study, which could allow a prompter response against emerging pathogens in agroecosystems worldwide.

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Genome-Wide Identification of MYB Transcription Factors and Screening of Members Involved in Stress Response in Actinidia

Xia

Liu

Lin

et al. 2022

IJMS

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MYB transcription factors (TFs) play an active role in plant responses to abiotic stresses, but they have not been systematically studied in kiwifruit (Actinidia chinensis). In this study, 181 AcMYB TFs were identified from the kiwifruit genome, unevenly distributed on 29 chromosomes. The high proportion (97.53%) of segmental duplication events (Ka/Ks values less than 1) indicated that AcMYB TFs underwent strong purification selection during evolution. According to the conservative structure, 91 AcR2R3-MYB TFs could be divided into 34 subgroups. A combination of transcriptomic data under drought and high temperature from four AcMYB TFs (AcMYB2, AcMYB60, AcMYB61 and AcMYB102) was screened out in response to stress and involvement in the phenylpropanoid pathway. They were highly correlated with the expression of genes related to lignin biosynthesis. qRT-PCR analysis showed that they were highly correlated with the expression of genes related to lignin biosynthesis in different tissues or under stress, which was consistent with the results of lignin fluorescence detection. The above results laid a foundation for further clarifying the role of MYB in stress.

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Expression analysis of defense-related genes in wild kiwifruit (Actinidia rufa) tolerant to bacterial canker

Cited by 4 publications

References 31 publications

Functional Analysis of a Methyltransferase Involved in Anthocyanin Biosynthesis from Blueberries (Vaccinium corymbosum)

Functional Analysis of a Methyltransferase Involved in Anthocyanin Biosynthesis from Blueberries (Vaccinium corymbosum)

Mitigation of Emergent Bacterial Pathogens Using Pseudomonas syringae pv. actinidiae as a Case Study—From Orchard to Gene and Everything in Between

Genome-Wide Identification of MYB Transcription Factors and Screening of Members Involved in Stress Response in Actinidia

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