Expression analysis of an APETALA1/FRUITFULL-like gene in Phalaenopsis sp. ‘Hatsuyuki’ (Orchidaceae)

Abstract

Members of the APETALA1 (AP1)/FRUITFULL (FUL)-like gene family of MADS-box genes play important roles in controlling the development of floral organs. To understand the molecular mechanisms of floral development in orchid, we isolated and characterized a Phalaenopsis AP1/FUL-like gene, PhalFUL. The results of phylogenetic analysis indicated that PhalFUL is in the monocots group of AP1/FUL-like gene. PhalFUL transcripts were detected in the flower buds, but not in vegetative organs. Moreover, in situ hybridization experiments revealed PhalFUL hybridization signals in all floral organ primordia at a very early stage of floral development, and continued expression in the column of whorls 3 and 4 until late developmental stages. These expression patterns were similar to those of the FUL-like genes in Arabidopsis (FUL) and Antirrhinum (DEFH28), suggesting that the PhalFUL is similar in function to FUL and DEFH28. Additional key words: A-class, AP1/FUL-like gene, floral organs Hort.

DOAP1 Promotes Flowering in the Orchid Dendrobium Chao Praya Smile

“…So far AP1 orthologs have been isolated in a wide range of plant species, such as pea (Pisum sativum) ( Berbel et al, 2001 ), apple (Malus domestica) ( Kotoda et al, 2002 ), common wheat ( Triticum aestivum ) ( Adam et al, 2007 ), moth orchid ( Phalaenopsis ‘Hatsuyuki’) ( Song et al, 2011 ), longan ( Dimocarpus longan) ( Winterhagen et al, 2013 ), trifoliate orange ( Poncirus trifoliata L. Raf.)…”

Integrated proteomic, transcriptomic, and metabolomic profiling reveals that the gibberellin–abscisic acid hub runs flower development in the Chinese orchid Cymbidium sinense

“…The ap1 mutation in Arabidopsis causes the absence of petals, petal transformation into bract-like structures, and the production of axillary flowers [ 25 ]; however, its overexpression causes early flowering and transformation of shoot apical meristem into floral meristem [ 26 ]. So far, AP1 orthologs have been identified in a number of species, such as apple [ 27 ], pea [ 27 ], longan [ 28 ], common wheat [ 29 ], birch [ 30 ], trifoliate orange [ 31 ], poplar [ 32 ], and moth orchid [ 33 ]. Conserved function of AP1 orthologs in the flowering process and floral organ formation has been found in different plants.…”