Expression analysis in response to heat stress in moth bean (Vigna aconitifolia (Jacq.) Marechal)

Bhavana, T; Shahina, K; Pooja, B; Sanjay, Kumar; Kumari, Ratna; Barman, Paramananda; Bhat, K. V.

doi:10.5958/0975-928x.2017.00185.5

Cited by 3 publications

(4 citation statements)

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“…4,15 The expression of genes for abiotic stress responsiveness was carried out by various gene expression studies in legumes. 3,13,16 Similarly, in the present study, the expression characterization of selected genes was done by semi-qRT PCR analysis. This was done to select the gene which shows the exponential up-regulation during the time course experiment of drought in moth bean.…”

Section: Assessment Of Drought Stress-responsive Expression Of Moth B...mentioning

confidence: 98%

“…Moth bean possesses resistance to extreme temperature regimes. 3,14 Our experiment conducted on moth bean during withholding of water and grown at 35 ºC has shown that these seedlings can efficiently live and perform their metabolic activity. Thus, owing to resistance against heat and drought stress, it is quite imperative to identify and characterize genes related to drought stress tolerance mechanisms at the molecular level.…”

Section: Assessment Of Drought Stress-responsive Expression Of Moth B...mentioning

confidence: 98%

“…Few studies have been brought to understand the drought tolerant mechanism in moth bean. 12,2,3,4 However, molecular cloning and characterization of stressresponsive genes have not been reported in moth bean. Thus, in order to combat the adverse effects of drought, it is very essential to develop water-deficit stress-tolerant genotypes.…”

Section: Journal Of Agriculture and Ecology | Research Articlementioning

confidence: 99%

“…Since, moth bean is a drought-hardy pulse thus it can prove the usefulness of drought stress tolerance mechanisms. 2,3,4 Additionally, the moth bean is a native crop of hot and dry habitats of northern and western parts of India. In arid and semi-arid regions drought as abiotic stress, is one of the major factors limiting plant growth at various stages of their life.…”

Section: Introductionmentioning

confidence: 99%

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Identification and characterization of CAT1 gene during drought stress in moth bean [Vigna aconitifolia (Jacq.) Marechal]

Meena¹,

Dogra²,

Sudarsan³

et al. 2022

J. Agric. Ecol.

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Moth bean [Vigna aconitifolia (Jacq.) Marechal] is a drought hardy orphan pulse crop. However, the information related to the identification and characterization of drought stress tolerance genes is very limited. Therefore, the present investigation was formulated to identified and characterized drought tolerant gene(s) from moth bean. Five genes were selected from available expression studies of moth bean and their expression pattern was assessed during time course experiment of drought stress in moth bean. During the time course experiment of drought stress in moth bean, the catalase1 (CAT1) gene was exponentially expressed in up-regulated manner. Thus, CAT1 gene of moth bean was identified as potential candidate gene and validated through Sanger’s sequencing. The genomic sequence CAT1 gene was named as VacoCAT1 and was further characterized using various bioinformatics tools. The VacoCAT1 showed an ORF with incomplete length of 213 bp which encoded 71 amino acids. The coding sequence of VacoCAT1 gene was shown a single exon due to incomplete nature of genomic sequences. The multiple sequence alignment of VacoCAT1 revealed the highly conserved region at 3’ site of the gene as compared to CAT1 gene of other crop species including legumes. The phylogenetic analysis of VacoCAT1 and CAT1 gene of other crop species including legumes revealed three clusters. The cluster VacoCAT1 gene showed close proximity with V. radiata CAT1 in cluster one of phylogenetic tree. The identified and characterized VacoCAT1 gene can be utilized as a genomic resource for enhance drought tolerance in susceptible pulses as well as other crops.

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