Expression analysis and prognostic significance of human kallikrein 11 in prostate cancer

Stavropoulou, Panagiota; Gregorakis, Alkiviades K.; Plebani, Mario; Scorilas, Andreas

doi:10.1016/j.cccn.2005.03.026

Cited by 38 publications

(25 citation statements)

References 27 publications

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“…The hK11 (also known as hippostatin) is a member of the kallikrein family of secreted serine proteases, which includes PSA (hK3). In previous work, hK11 mRNA was found to be over-expressed in 43/66 (65%) of prostate cancer tissue samples [28], and isoform 2 protein was shown to have an increased abundance in the serum of 60% of men with prostate cancer compared with normal [24]. Over-expression in the tumor environment could be responsible for the generation of an immune response against the protein, as observed in this work.…”

Section: Discussionsupporting

confidence: 68%

An experimental strategy for quantitative analysis of the humoral immune response to prostate cancer antigens using natural protein microarrays

Forrester

Qiu

Mangold

et al. 2007

Proteomics Clinical Apps

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The identification of human tumor antigens has potential utility in the diagnosis and treatment of cancers. We demonstrate here a complete strategy to profile immunoreactivity and identify tumor antigens from proteins derived from tumor cell lines. Microarrays of proteins produced from 2-D LC fractionation of prostate tumor cell-line lysates were used to profile immunoreactivity in the sera of prostate cancer patients and control subjects. Cancer-associated immunoreactivity to distinct groups of chromatography fractions was present in about 50% of the patients, with greater immunoreactivity present in patients with non-organ-confined cancer than in patients with organ-confined cancer. We grouped the immunoreactive fractions by similarities in elution order and patterns of immunoreactivity to guide and interpret the MS analysis of selected fractions, which was used to identify the proteins that may be responsible for the immunoreactivity. As a complementary method to further characterize and validate the immunoreactivity of the proteins identified by mass spectrometry, we demonstrate the use of focused microarrays of recombinant proteins. Disease-associated immunoreactivity was confirmed for one of the identified proteins, human Kallikrein 11. These results demonstrate a practical approach to screening, identifying, and validating immunoreactive proteins that could be applied to diverse studies on humoral immune responses.

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Section: Discussionsupporting

confidence: 68%

An experimental strategy for quantitative analysis of the humoral immune response to prostate cancer antigens using natural protein microarrays

Forrester

Qiu

Mangold

et al. 2007

Proteomics Clinical Apps

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“…Although there are studies that point to its potential as PCa marker, there is insignificant information regarding KLK4 expression in cancer and benign tissue [159,160]. In addition to these genes, there are also reports that KLK5 [161,162], KLK11 [163,164], KLK14 [165,166] and KLK15 [167] are expressed in the prostate and might be of value as diagnostic or prognostic biomarkers in PCa. What about the utility of kallikrein-related peptidases as biomarkers in tumors other than PCa?…”

Section: Kallikrein-related Peptidases As Tumor Markersmentioning

confidence: 96%

Kallikrein-related peptidases

Lundwall¹,

Brattsand²

2008

Cell. Mol. Life Sci.

131

111

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Kallikrein 1 (KLK1), a key component of the kallikrein-kinin system, originates from a locus on the long arm of chromosome 19 that contains several related serine endopeptidases. The biological role of these kallikrein-related peptidases is not clear, but emerging evidence suggests that they might be important in several physiological systems, e.g., in male reproduction, skin homeostasis, tooth enamel formation and neural development and plasticity. The kallikrein locus has undergone some major evolutionary events. Most spectacular are relatively recent duplications of KLK1 that have created 13 and 9 functional genes that are unique to the mouse and the rat, respectively. Human paralogs are KLK2 and KLK3: the latter encoding the cancer biomarker prostate-specific antigen. In this review on kallikrein-related peptidases, the focus is on their evolution, their role in skin homeostasis and semen liquefaction, and their utility as cancer biomarkers.

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“…It is under steroid hormone regulation in cancer cell lines and expressed in two alternative splicing isoforms [9,10]. All of the KLKs are thought to possess serine protease catalytic activity and may be able to activate each other as well as other molecules, such as growth factors and cytokines, in a cascade of events associated with tumorigenesis [11].…”

Section: Discussionmentioning

confidence: 99%

Over-expression of human kallikrein 11 is associated with poor prognosis in patients with low rectal carcinoma

Tang

et al. 2009

Med Oncol

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Expression analysis and prognostic significance of human kallikrein 11 in prostate cancer

Cited by 38 publications

References 27 publications

An experimental strategy for quantitative analysis of the humoral immune response to prostate cancer antigens using natural protein microarrays

An experimental strategy for quantitative analysis of the humoral immune response to prostate cancer antigens using natural protein microarrays

Kallikrein-related peptidases

Over-expression of human kallikrein 11 is associated with poor prognosis in patients with low rectal carcinoma

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