Expression analysis and functional characterization of a cold-responsive gene COR15A from Arabidopsis thaliana

Liu, Dong; Li, Weichun; Cheng, Jianfeng; Hou, Ling

doi:10.1007/s11738-014-1615-8

Cited by 9 publications

(3 citation statements)

References 57 publications

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“…Transgenic plants over‐expressing AtGSTU19 activated the expression of some late stress‐response genes, such as RD29A , COR15A and KIN1 , even under normal growth conditions. The three genes were sensitive to various abiotic stressors and were induced by dehydration, high salinity or low temperature (Wang et al , Msanne et al , Liu et al ). Our studies showed that plants over‐expressing AtGSTU19 may activate stress response pathways to a certain degree under normal conditions.…”

Section: Discussionmentioning

confidence: 99%

Over‐expression of AtGSTU19 provides tolerance to salt, drought and methyl viologen stresses in Arabidopsis

Tian

Xing

et al. 2015

Physiologia Plantarum

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The plant-specific tau class of glutathione S-transferases (GSTs) is often highly stress-inducible and expressed in a tissue-specific manner, thereby suggesting its important protective roles. Although activities associated with the binding and transport of reactive metabolites have been proposed, little is known about the regulatory functions of GSTs. Expression of AtGSTU19 is induced by several stimuli, but the function of this GST remains unknown. In this study, we demonstrated that transgenic over-expressing (OE) plants showed enhanced tolerance to different abiotic stresses and increased percentage of seed germination and cotyledon emergence. Transgenic plants exhibited an increased level of proline and activities of antioxidant enzymes, along with decreased malonyldialdehyde level under stress conditions. Real-time polymerase chain reaction (PCR) analyses revealed that the expression levels of several stress-regulated genes were altered in AtGSTU19 OE plants. These results indicate that AtGSTU19 plays an important role in tolerance to salt/drought/methyl viologen stress in Arabidopsis.

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Section: Discussionmentioning

confidence: 99%

Over‐expression of AtGSTU19 provides tolerance to salt, drought and methyl viologen stresses in Arabidopsis

Tian

Xing

et al. 2015

Physiologia Plantarum

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“…Changes in plastid gene expression (PGE) and the photosynthetic electron transport chain (PET) also trigger chloroplast-to-nucleus retrograde signalling 19 21 23 . Several recent studies have investigated the role of retrograde signalling in regulating the expression of nuclear genes during several stresses, including high light, drought, low temperature, heat, and excess ammonium 24 25 26 27 28 29 . Therefore, the disruption of the functional and developmental state of the chloroplast by endogenous factors or exogenous environmental cues generates a wide variety of chloroplast retrograde signals that regulate the expression of many nuclear genes encoding chloroplast-localized proteins.…”

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confidence: 99%

The novel protein DELAYED PALE-GREENING1 is required for early chloroplast biogenesis in Arabidopsis thaliana

Liu

Cheng

2016

Sci Rep

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Chloroplast biogenesis is one of the most important subjects in plant biology. In this study, an Arabidopsis early chloroplast biogenesis mutant with a delayed pale-greening phenotype (dpg1) was isolated from a T-DNA insertion mutant collection. Both cotyledons and true leaves of dpg1 mutants were initially albino but gradually became pale green as the plant matured. Transmission electron microscopic observations revealed that the mutant displayed a delayed proplastid-to-chloroplast transition. Sequence and transcription analyses showed that AtDPG1 encodes a putatively chloroplast-localized protein containing three predicted transmembrane helices and that its expression depends on both light and developmental status. GUS staining for AtDPG1::GUS transgenic lines showed that this gene was widely expressed throughout the plant and that higher expression levels were predominantly found in green tissues during the early stages of Arabidopsis seedling development. Furthermore, quantitative real-time RT-PCR analyses revealed that a number of chloroplast- and nuclear-encoded genes involved in chlorophyll biosynthesis, photosynthesis and chloroplast development were substantially down-regulated in the dpg1 mutant. These data indicate that AtDPG1 plays an essential role in early chloroplast biogenesis, and its absence triggers chloroplast-to-nucleus retrograde signalling, which ultimately down-regulates the expression of nuclear genes encoding chloroplast-localized proteins.

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“…qRT-PCR was performed on the Applied Biosystems 7500 real time PCR System using SYBR Green/ROX Master Mix (Thermo Scientific). The primers used for qRT-PCR examination of RD29A , ABF3 , P5CS , COR15A , and ACT2 have been described previously ( Strizhov et al, 1997 ; Nakashima et al, 2006 ; Liu et al, 2014 , 2015 ; Lu et al, 2015 ; Wang et al, 2015 ). Other primers used for qRT-PCR were: ABI5 , 5′-GGAGATTGCGGACATTG-ATGAG-3′ and 5′-GGGAACACTAGTAAAGCAGATC-3′, ABF4 , 5′-ACTGGAAGCCGAAATTGAAAAGCTC-3′ and 5′-CACCATGGTCCGGTTAATGTCCT-3′, ERF95 , 5′-CCAT-TCTCAATTTTCCTCAC-3′ and 5′-AACTCAATAACTTCC-CTCCC-3′, ERF96 , 5′-GCGGCTAGAGCCTATG-3′ and 5′-GTACTTG GATGATAGTG-3′, ERF97 , 5′-ACCGTGGAGT-AAGGAGAC-3′ and 5′-GAAGTTGAGAATGGCAGC-3′, ERF98 , 5′-GGAGCAGCAACAACCAAT-3′ and 5′-AGCGAG-ATGACCCCTAAG-3′.…”

Section: Methodsmentioning

confidence: 99%

The Small Ethylene Response Factor ERF96 is Involved in the Regulation of the Abscisic Acid Response in Arabidopsis

et al. 2015

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Ethylene regulates many aspects of plant growth and development including seed germination, leaf senescence, and fruit ripening, and of plant responses to environmental stimuli including both biotic and abiotic stresses. Ethylene response factors (ERFs) are plant-specific transcription factors and are a subfamily of the AP2 (APETALA2)/ERF transcription factor family. The function of many members in this large gene family remains largely unknown. ERF96, a member of the Group IX ERF family transcription factors, has recently been shown to be a transcriptional activator that is involved in plant defense response in Arabidopsis. Here we provide evidence that ERF96 is a positive regulator of abscisic acid (ABA) responses. Bioinformatics analysis indicated that there are a total four small ERFs in Arabidopsis including ERF95, ERF96, ERF97, and ERF98, and that ERF96 forms a cluster with ERF95 and ERF97. By using quantitative RT-PCR, we found that ERF96 is expressed in all tissues and organs examined except roots, with relatively high expression in flowers and seeds. Results from the protoplast transfection assay indicated that the EDLL motif-containing C-terminal domain is responsible for ERF96’s transcriptional activity. Although loss-of-function mutant of ERF96 was morphologically similar to wild type plants, transgenic plants overexpressing ERF96 had smaller rosette size and were delayed in flowering time. In ABA sensitivity assays, we found that ERF96 overexpression plants were hypersensitive to ABA in terms of ABA inhibition of seed germination, early seedling development and root elongation. Consistent with these observations, elevated transcript levels of some ABA-responsive genes including RD29A, ABI5, ABF3, ABF4, P5CS, and COR15A were observed in the transgenic plants in the presence of ABA. However, in the absence of ABA treatment, the transcript levels of these ABA-responsive genes remained largely unchanged. Our experiments also showed that water loss in ERF96 overexpression plants was slower than that in Col wild type plants. Stomatal closure assays indicated that ERF96 overexpression plants had reduced stomatal aperture in the presence of ABA. Taken together, our results suggest that ERF96 positively regulates ABA responses in Arabidopsis.

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Expression analysis and functional characterization of a cold-responsive gene COR15A from Arabidopsis thaliana

Cited by 9 publications

References 57 publications

Over‐expression of AtGSTU19 provides tolerance to salt, drought and methyl viologen stresses in Arabidopsis

Over‐expression of AtGSTU19 provides tolerance to salt, drought and methyl viologen stresses in Arabidopsis

The novel protein DELAYED PALE-GREENING1 is required for early chloroplast biogenesis in Arabidopsis thaliana

The Small Ethylene Response Factor ERF96 is Involved in the Regulation of the Abscisic Acid Response in Arabidopsis

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