Expression analyses of PLEKHG2, a Rho family-specific guanine nucleotide exchange factor, during mouse brain development

Nishikawa, Masashi; Ito, Hidenori; Noda, Mariko; Hamada, Naoki; Tabata, Hidenori; Nagata, Koh‐ichi

doi:10.1007/s00795-020-00275-1

Cited by 8 publications

(14 citation statements)

References 18 publications

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“…Sample preparation was performed as described [10-12]. Briefly, ICR adult mice were purchased from Japan SLC (Shizuoka, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…Protein concentration was estimated using BCA protein assay reagent kit (Pierce, Rockford, IL, USA) with bovine serum albumin as a standard. SDS-PAGE (10% gel) and immunoblotting was conducted as described ([10]).…”

Section: Methodsmentioning

confidence: 99%

“…Sample preparation was performed as described [10, 13]. Briefly, adult mouse brains were homogenized in 5 mM HEPES (pH 7.4) containing 1 mM MgCl 2 , 0.5 mM CaCl 2 , phosphatase inhibitors, and the protease inhibitor mix with a Teflon homogenizer.…”

Section: Methodsmentioning

confidence: 99%

“…Mouse brains of either sex fixed with 4% paraformaldehyde at postnatal day 2 or 18 were cut into coronal or sagittal sections (14 μm thickness) with a cryostat (Leica CM1900, Leica Microsystems, Wetzlar, Germany). After blocking in a bovine serum albumin-based blocking buffer (0.1% [w/v] in PBS with 0.5% [v/v] Triton X-100), sections were treated with HistoVT One (Nacalai Tesque, Kyoto, Japan) and then processed for immunohistochemistry as reported previously [10]. Images were captured using LSM-880 confocal laser microscope (Carl Zeiss, Germany).…”

Section: Methodsmentioning

confidence: 99%

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Expression Analyses of Rac3, a Rho Family Small GTPase, during Mouse Brain Development

et al. 2021

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Rac3 is a member of Rho family small GTPases which regulates cellular signaling and cytoskeletal dynamics. The RAC3 gene abnormalities have been shown to cause neurodevelopmental disorders with structural brain anomalies, including polymicrogyria/dysgyria, callosal abnormalities, brainstem anomalies, and cerebellar dysplasia. Although this evidence indicates that Rac3 is essential in brain development, not only its molecular mechanism but also the expression profile is yet to be elucidated. In this study, we carried out expression analyses of Rac3 with mouse brain tissues. In immunoblotting, Rac3 exhibited a tissue-dependent expression profile in the young adult mouse and was expressed in a developmental stage-dependent manner in brain. In primary cultured hippocampal neurons, while Rac3 was distributed mainly in the cytoplasm, it was visualized in axon and dendrites with partial localization at synapses, in consistent with the observation in biochemical fractionation analyses. In immunofluorescence analyses with brain slices, Rac3 was distributed strongly and moderately in the axon and cytoplasm, respectively, of cerebral cortex at postnatal day (P) 2 and P18. Similar distribution profile was also observed in hippocampus. Taken together, the results obtained strongly suggest that Rac3 plays an important physiological role in neuronal tissues during corticogenesis, and defects in the Rac3 function induce structural brain anomalies leading to pathogenesis of neurodevelopmental disorders.

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“…Sample preparation was performed as described [10-12]. Briefly, ICR adult mice were purchased from Japan SLC (Shizuoka, Japan).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Expression Analyses of Rac3, a Rho Family Small GTPase, during Mouse Brain Development

et al. 2021

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“…Intriguingly, FHL1 may also modulate GPCR signaling by binding to Pleckstrin homology and RhoGEF domain containing G2 (Plekhg2) (Sato et al 2016 ), a Rho family-specific guanine nucleotide exchange factor that is known to associate with small G-proteins Gβɣ and Gsα (Sugiyama et al 2017 ). While Plekhg2 is known to be expressed in heart or skeletal muscles (Nishikawa et al 2021 ), its role in modulating FHL1-dependent signaling in cross-striated muscles has not been further investigated. The linkage of FHL1 gene activity to GPCR signaling suggests a positive feedback loop that may be modulated by actions of FHL1 itself.…”

Section: Fhl Proteins and Protein Kinases As Regulators For Titin-based Signaling And Stiffnessmentioning

confidence: 99%

The titin N2B and N2A regions: biomechanical and metabolic signaling hubs in cross-striated muscles

et al. 2021

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Muscle specific signaling has been shown to originate from myofilaments and their associated cellular structures, including the sarcomeres, costameres or the cardiac intercalated disc. Two signaling hubs that play important biomechanical roles for cardiac and/or skeletal muscle physiology are the N2B and N2A regions in the giant protein titin. Prominent proteins associated with these regions in titin are chaperones Hsp90 and αB-crystallin, members of the four-and-a-half LIM (FHL) and muscle ankyrin repeat protein (Ankrd) families, as well as thin filament-associated proteins, such as myopalladin. This review highlights biological roles and properties of the titin N2B and N2A regions in health and disease. Special emphasis is placed on functions of Ankrd and FHL proteins as mechanosensors that modulate muscle-specific signaling and muscle growth. This region of the sarcomere also emerged as a hotspot for the modulation of passive muscle mechanics through altered titin phosphorylation and splicing, as well as tethering mechanisms that link titin to the thin filament system.

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Expression analyses of WAC, a responsible gene for neurodevelopmental disorders, during mouse brain development

Nishikawa¹,

Matsuki²,

Hamada³

et al. 2023

Med Mol Morphol

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Expression analyses of PLEKHG2, a Rho family-specific guanine nucleotide exchange factor, during mouse brain development

Cited by 8 publications

References 18 publications

Expression Analyses of Rac3, a Rho Family Small GTPase, during Mouse Brain Development

Expression Analyses of Rac3, a Rho Family Small GTPase, during Mouse Brain Development

The titin N2B and N2A regions: biomechanical and metabolic signaling hubs in cross-striated muscles

Expression analyses of WAC, a responsible gene for neurodevelopmental disorders, during mouse brain development

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