Expressing the sweet potato orange gene in transgenic potato improves drought tolerance and marketable tuber production

Cho, Kwang-Soo; Han, Eun-Heui; Kwak, Sang‐Soo; Cho, Ji-Hong; Im, Ju-Seong; Hong, Seung Ho; Sohn, Hwang Bae; Kim, Yun-Hee; Lee, Shin-Woo

doi:10.1016/j.crvi.2016.04.010

Cited by 30 publications

(17 citation statements)

References 27 publications

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“…These findings suggest that Or is a multi-functional protein. Interestingly, recent work showed that transgenic sweetpotato calli, Arabidopsis , alfalfa, and potato overexpressing IbOr accumulate carotenoids and maintain higher photosystem II (PSII) efficiency and chlorophyll content under abiotic stress (Kim et al, 2013; Park et al, 2015, 2016; Wang et al, 2015; Cho et al, 2016). However, the mechanisms underlying the effects of Or on carotenoid biosynthesis and the abiotic stress response are currently unknown.…”

Section: Introductionmentioning

confidence: 99%

IbOr Regulates Photosynthesis under Heat Stress by Stabilizing IbPsbP in Sweetpotato

et al. 2017

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The Orange (Or) protein regulates carotenoid biosynthesis and environmental stress in plants. Previously, we reported that overexpression of the sweetpotato [Ipomoea batatas (L.) Lam] Or gene (IbOr) in transgenic Arabidopsis (referred to as IbOr-OX/At) increased the efficiency of photosystem II (PSII) and chlorophyll content after heat shock. However, little is known about the role of IbOr in PSII-mediated protection against abiotic stress. In this study, comparative proteomics revealed that expression of PsbP (an extrinsic subunit of PSII) is up-regulated in heat-treated IbOr-OX/At plants. We then identified and functionally characterized the PsbP-like gene (IbPsbP) from sweetpotato. IbPsbP is predominantly localized in chloroplast, and its transcripts are tissue-specifically expressed and up-regulated in response to abiotic stress. In addition, IbOr interacts with IbPsbP and protects it from heat-induced denaturation, consistent with the observation that transgenic sweetpotato overexpressing IbOr maintained higher PSII efficiency and chlorophyll content upon exposure to heat stress. These results indicate that IbOr can protect plants from environmental stress not only by controlling carotenoid biosynthesis but also by directly stabilizing PSII.

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Section: Introductionmentioning

confidence: 99%

IbOr Regulates Photosynthesis under Heat Stress by Stabilizing IbPsbP in Sweetpotato

et al. 2017

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“…Expression levels of some genes encoding for plant DnaJ which are targeted to the chloroplast are induced by different abiotic stresses [38][39][40] . In addition, in sweet potato Or expression was differentially affected by salt, drought, oxidative stress and heat stress, depending on the analyzed tissue 10,[41][42][43] . In sweet potato, Or was downregulated by heat stress in leaves, as the case of CsOr-a and CsOr-b in heat-treated stigmas.…”

Section: Discussionmentioning

confidence: 99%

Differential interaction of Or proteins with the PSY enzymes in saffron

Ahrazem

López

Argandoña

et al. 2020

Sci Rep

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Colored apocarotenoids accumulate at high concentrations in few plant species, where display a role in attraction of pollinators and seed dispersers. Among these apocarotenoids, crocins accumulate at high concentrations in the stigma of saffron and are responsible for the organoleptic and medicinal properties of this spice. Phytoene synthase and Orange protein are key for carotenoid biosynthesis and accumulation. We previously isolated four phytoene synthase genes from saffron with differential roles in carotenoid and apocarotenoid biosynthesis. However, the implications of Orange genes in the regulation of apocarotenoid accumulation are unknown. Here, we have identified two Orange genes from saffron, with different expression patterns. CsOr-a was mainly expressed in vegetative tissues and was induced by light and repressed by heat stress. Both CsOr-a and CsOr-b were expressed in stigmas but showed a different profile during the development of this tissue. The interactions of CsOr-a and CsOr-b were tested with all the four phytoene synthase proteins from saffron and with CsCCD2. None interactions were detected with CCD2 neither with the phytoene synthase 2, involved in apocarotenoid biosynthesis in saffron. The obtained results provide evidence of different mechanisms regulating the phytoene synthase enzymes in saffron by Orange for carotenoid and apocarotenoid accumulation in saffron.Nucleic acid purification and cDNA isolation. Total RNA was isolated from red stigmas 48 , following the manufacturer's protocols (Qiagen, Hilden, Germany). First-strand cDNAs were synthesized by RT from 2 µg of total RNA using an 18-base pair oligo dT primer and a first-strand cDNA synthesis kit (GE Healthcare Life Sciences, www.gelifesciences.com) according to manufacturer's instructions. These cDNAs were used as templates for PCR using specific primers for CsOr-a and CsOr-b (Suppl . Table 1). Thermal cycling parameters were 2 min at 95 °C, 35 cycles of 20 s at 95 °C, 20 s at 60 °C and 1 min 30 s at 72 °C, followed by a final extension of 5 min at 72 °C. The PCR products were separated in a 1% agarose gel, purified, ligated into pGEM-T (Promega, www. promega.com), and then introduced into E. coli. Scientific RepoRtS |(2020) 10:552 | https://doi.

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“…In addition, transgenic alfalfa and potato plants overexpressing IbOr were generated and characterized (Cho et al 2016, Goo et al 2015, Wang et al 2015). Transgenic alfalfa and potato plants exhibit elevated tolerance to various abiotic stresses including oxidative, salt and drought stress, as well as higher carotenoid content (Goo et al 2015, Wang et al 2015).…”

Section: Accumulation Of Carotenoids By Ibor Genementioning

confidence: 99%

Metabolic engineering of carotenoids in transgenic sweetpotato

Kang

Park

et al. 2017

Breed. Sci.

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Sweetpotato [Ipomoea batatas (L.) Lam], which contains high levels of antioxidants such as ascorbate and carotenoids in its storage root, is one of the healthiest foods, as well as one of the best starch crops for growth on marginal lands. In plants, carotenoid pigments are involved in light harvesting for photosynthesis and are also essential for photo-protection against excess light. As dietary antioxidants in humans, these compounds benefit health by alleviating aging-related diseases. The storage root of sweetpotato is a good source of both carotenoids and carbohydrates for human consumption. Therefore, metabolic engineering of sweetpotato to increase the content of useful carotenoids represents an important agricultural goal. This effort has been facilitated by cloning of most of the carotenoid biosynthetic genes, as well as the Orange gene involved in carotenoid accumulation. In this review, we describe our current understanding of the regulation of biosynthesis, accumulation and catabolism of carotenoids in sweetpotato. A deeper understanding of these topics should contribute to development of new sweetpotato cultivars with higher levels of nutritional carotenoids and abiotic stress tolerance.

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Expressing the sweet potato orange gene in transgenic potato improves drought tolerance and marketable tuber production

Cited by 30 publications

References 27 publications

IbOr Regulates Photosynthesis under Heat Stress by Stabilizing IbPsbP in Sweetpotato

IbOr Regulates Photosynthesis under Heat Stress by Stabilizing IbPsbP in Sweetpotato

Differential interaction of Or proteins with the PSY enzymes in saffron

Metabolic engineering of carotenoids in transgenic sweetpotato

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