Expressing AtNHX1 in barley (Hordium vulgare L.) does not improve plant performance under saline conditions

Adem, Getnet Dino; Roy, Stuart J.; Plett, Darren; Zhou, Meixue; Bowman, John P.; Shabala, Sergey

doi:10.1007/s10725-015-0063-9

Cited by 22 publications

(17 citation statements)

References 51 publications

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“…Similar observation was also reported in barley (Hordeum vulgare L.) expressing AtNHX1, which showed accumulation of Na + and K + (Adem et al, 2015). The greater tolerance of the transgenic soybean in our studies could be attributed to ion homeostasis sustainability resulting from greater accumulation of Na + in root and leaf tissues by the expression of AtNHX1 and AtAVP1, which have been reported in Arabidopsis, tomato, sugar beet, and barley (Gaxiola et al, 2001;Bhaskaran and Savithramma, 2011;Adem et al, 2015;Wu et al, 2015). The increased accumulation of Na + was possibly a result of the activity of the vacuolar Na + / H + antiporter AtNHX1 and the increased sequestration of Na + in the vacuole and enhanced vacuolar osmoregulatory capacity of H + pyrophosphatase in the transgenic plants.…”

Section: Transgenic Soybean Did Not Outperform the Wild Type In Long-supporting

confidence: 88%

“…Co-expression of xerophyte AVP1 and NHX1 in sugar beet showed greater accumulation of Na + than nontransgenic plants under 8 d of exposure to 400 mM NaCl stress (Wu et al, 2015). Similar observation was also reported in barley (Hordeum vulgare L.) expressing AtNHX1, which showed accumulation of Na + and K + (Adem et al, 2015). The greater tolerance of the transgenic soybean in our studies could be attributed to ion homeostasis sustainability resulting from greater accumulation of Na + in root and leaf tissues by the expression of AtNHX1 and AtAVP1, which have been reported in Arabidopsis, tomato, sugar beet, and barley (Gaxiola et al, 2001;Bhaskaran and Savithramma, 2011;Adem et al, 2015;Wu et al, 2015).…”

Section: Transgenic Soybean Did Not Outperform the Wild Type In Long-supporting

confidence: 83%

“…Targeting stresssignaling regulators was also beneficial to improve salt tolerance. Similar observation was also found in barley where the transgenic plants expressing AtNHX1 had no effect on tolerance (Adem et al, 2015). Together, these studies suggest that it is possible to improve salt tolerance in soybean by genetic engineering.…”

Section: Transgenic Soybean Did Not Outperform the Wild Type In Long-supporting

confidence: 81%

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Co‐expression of Arabidopsis AtAVP1 and AtNHX1 to Improve Salt Tolerance in Soybean

Nguyen

et al. 2019

Crop Science

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Section: Transgenic Soybean Did Not Outperform the Wild Type In Long-supporting

confidence: 88%

Section: Transgenic Soybean Did Not Outperform the Wild Type In Long-supporting

confidence: 83%

Section: Transgenic Soybean Did Not Outperform the Wild Type In Long-supporting

confidence: 81%

See 1 more Smart Citation

Co‐expression of Arabidopsis AtAVP1 and AtNHX1 to Improve Salt Tolerance in Soybean

Nguyen

et al. 2019

Crop Science

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“…The NHX1 proteins may operate principally as K + rather than Na + exchangers (Bassil et al 2011;Barragan et al 2012), with their major role being the regulation of cytosolic K + or pH. Furthermore, transcriptional changes in NHX1 and NHX2 expression were not correlated with salt tolerance in three barley cultivars (Adem et al 2014), and overexpression of AtNHX1 in barley did not improve its salt tolerance (Adem et al 2015). This may be because these proteins are regulated post-translationally.…”

Section: Genes Involved In Tissue Tolerance -Can These Provide a Molementioning

confidence: 99%

Tissue tolerance: an essential but elusive trait for salt-tolerant crops

Munns

James

Gilliham

et al. 2016

Functional Plant Biol.

172

139

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Abstract. For a plant to persist in saline soil, osmotic adjustment of all plant cells is essential. The more salt-tolerant species accumulate Na + and Cl -to concentrations in leaves and roots that are similar to the external solution, thus allowing energy-efficient osmotic adjustment. Adverse effects of Na + and Cl -on metabolism must be avoided, resulting in a situation known as 'tissue tolerance'. The strategy of sequestering Na + and Cl -in vacuoles and keeping concentrations low in the cytoplasm is an important contributor to tissue tolerance. Although there are clear differences between species in the ability to accommodate these ions in their leaves, it remains unknown whether there is genetic variation in this ability within a species. This viewpoint considers the concept of tissue tolerance, and how to measure it. Four conclusions are drawn: (1) osmotic adjustment is inseparable from the trait of tissue tolerance; (2) energy-efficient osmotic adjustment should involve ions and only minimal organic solutes; (3) screening methods should focus on measuring tolerance, not injury; and (4) high-throughput protocols that avoid the need for control plants and multiple Na + or Cl -measurements should be developed. We present guidelines to identify useful genetic variation in tissue tolerance that can be harnessed for plant breeding of salt tolerance.

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“…Adem et al . () suggested that several genes need to be pyramided for antiporter transformation to achieve a meaningful effect. The meta‐analytic perspective across the literature provides some support for this idea, in that the TC‐induced improvement was largest for some plant characteristics when more than one gene was transferred to recipient plants relative to transformation with a single gene.…”

Section: Discussionmentioning

confidence: 99%

Increased salt tolerance with overexpression of cation/proton antiporter 1 genes: a meta‐analysis

Augé

Dong

et al. 2016

Plant Biotechnology Journal

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SummaryCation/proton antiporter 1 (CPA1) genes encode cellular Na+/H+ exchanger proteins, which act to adjust ionic balance. Overexpression of CPA1s can improve plant performance under salt stress. However, the diversified roles of the CPA1 family and the various parameters used in evaluating transgenic plants over‐expressing CPA1s make it challenging to assess the complex functions of CPA1s and their physiological mechanisms in salt tolerance. Using meta‐analysis, we determined how overexpression of CPA1s has influenced several plant characteristics involved in response and resilience to NaCl stress. We also evaluated experimental variables that favour or reduce CPA1 effects in transgenic plants. Viewed across studies, overexpression of CPA1s has increased the magnitude of 10 of the 19 plant characteristics examined, by 25% or more. Among the ten moderating variables, several had substantial impacts on the extent of CPA1 influence: type of culture media, donor and recipient type and genus, and gene family. Genes from monocotyledonous plants stimulated root K+, root K+/Na+, total chlorophyll, total dry weight and root length much more than genes from dicotyledonous species. Genes transformed to or from Arabidopsis have led to smaller CPA1‐induced increases in plant characteristics than genes transferred to or from other genera. Heterogeneous expression of CPA1s led to greater increases in leaf chlorophyll and root length than homologous expression. These findings should help guide future investigations into the function of CPA1s in plant salt tolerance and the use of genetic engineering for breeding of resistance.

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Expressing AtNHX1 in barley (Hordium vulgare L.) does not improve plant performance under saline conditions

Cited by 22 publications

References 51 publications

Co‐expression of Arabidopsis AtAVP1 and AtNHX1 to Improve Salt Tolerance in Soybean

Co‐expression of Arabidopsis AtAVP1 and AtNHX1 to Improve Salt Tolerance in Soybean

Tissue tolerance: an essential but elusive trait for salt-tolerant crops

Increased salt tolerance with overexpression of cation/proton antiporter 1 genes: a meta‐analysis

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