Expressing Arabidopsis thaliana V-ATPase subunit C in barley (Hordeum vulgare) improves plant performance under saline condition by enabling better osmotic adjustment

Adem, Getnet Dino; Roy, Stuart J.; Huang, Yuqing; Chen, Zhong‐Hua; Wang, Feifei; Zhou, Meixue; Bowman, John P.; Holford, Paul; Shabala, Sergey

doi:10.1071/fp17133

Cited by 21 publications

(11 citation statements)

References 65 publications

(77 reference statements)

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“…Contrary to no differences in Mott and Wang (2007), NHX1 and NHX2 expression levels varied between treatments and wheat lines ( Figure S5 ). Like Adem et al (2017), we found no correlation between expression levels of NHX genes and grain yield. However, other studies have found that increased expression of NHX genes can enhance growth under saline conditions (Apse et al, 1999; Zhang et al, 2001; Bayat et al, 2011).…”

Section: Discussioncontrasting

confidence: 66%

Bread Wheat With High Salinity and Sodicity Tolerance

et al. 2019

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Soil salinity and sodicity are major constraints to global cereal production, but breeding for tolerance has been slow. Narrow gene pools, over-emphasis on the sodium (Na+) exclusion mechanism, little attention to osmotic stress/tissue tolerance mechanism(s) in which accumulation of inorganic ions such as Na+ is implicated, and lack of a suitable screening method have impaired progress. The aims of this study were to discover novel genes for Na+ accumulation using genome-wide association studies, compare growth responses to salinity and sodicity in low-Na+ bread Westonia with Nax1 and Nax2 genes and high-Na+ bread wheat Baart-46, and evaluate growth responses to salinity and sodicity in bread wheats with varying leaf Na+ concentrations. The novel high-Na+ bread wheat germplasm, MW#293, had higher grain yield under salinity and sodicity, in absolute and relative terms, than the other bread wheat entries tested. Genes associated with high Na+ accumulation in bread wheat were identified, which may be involved in tissue tolerance/osmotic adjustment. As most modern bread wheats are efficient at excluding Na+, further reduction in plant Na+ is unlikely to provide agronomic benefit. The salinity and sodicity tolerant germplasm MW#293 provides an opportunity for the development of future salinity/sodicity tolerant bread wheat.

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Section: Discussioncontrasting

confidence: 66%

Bread Wheat With High Salinity and Sodicity Tolerance

et al. 2019

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“…Arabidopsis mutants lacking an H + -ATPase isoform showed increased sensitivity to salt and accumulate higher concentrations of Na + in leaves compared to wild type plants ( Vitart et al, 2001 ). On the contrary, expressing Arabidopsis thaliana V-ATPase subunit C in barley improved plant performance under saline condition by enabling better osmotic adjustment ( Adem et al, 2017 ). When comparing MP with waterlogging/salt tolerance scores from the same population, MP showed significant correlations with both waterlogging and salinity tolerance ( Figure 4 ).…”

Section: Discussionmentioning

confidence: 99%

Cell-Based Phenotyping Reveals QTL for Membrane Potential Maintenance Associated with Hypoxia and Salinity Stress Tolerance in Barley

et al. 2017

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Waterlogging and salinity are two major abiotic stresses that hamper crop production world-wide resulting in multibillion losses. Plant abiotic stress tolerance is conferred by many interrelated mechanisms. Amongst these, the cell’s ability to maintain membrane potential (MP) is considered to be amongst the most crucial traits, a positive relationship between the ability of plants to maintain highly negative MP and its tolerance to both salinity and waterlogging stress. However, no attempts have been made to identify quantitative trait loci (QTL) conferring this trait. In this study, the microelectrode MIFE technique was used to measure the plasma membrane potential of epidermal root cells of 150 double haploid (DH) lines of barley (Hordeum vulgare L.) from a cross between a Chinese landrace TX9425 and Japanese malting cultivar Naso Nijo under hypoxic conditions. A major QTL for the MP in the epidermal root cells in hypoxia-exposed plants was identified. This QTL was located on 2H, at a similar position to the QTL for waterlogging and salinity tolerance reported in previous studies. Further analysis confirmed that MP showed a significant contribution to both waterlogging and salinity tolerance. The fact that the QTL for MP was controlled by a single major QTL illustrates the power of the single-cell phenotyping approach and opens prospects for fine mapping this QTL and thus being more effective in marker assisted selection.

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“…The increased accumulation of Na + and K + in leaves in response to NaCl stress was also observed in transgenic S. lycopersicum M cv. Tianjinbaiguo overexpressing V-H + ATPase subunit B1 gene (MdVHA-B1) from M. domestica, although quantitative data on growth are lacking (Hu et al, 2015) and in H. vulgare overexpressing V-H + ATPase subunit C gene (AtVHA-C) from A. thaliana (Adem et al, 2017). Adem et al (2017) suggested that higher Na + (1.2-to 1.3-fold) and K + (1.3-to 1.5-fold) concentrations in leaves of transgenic H. vulgare grown in 300 mM NaCl, as compared to wild-type, may be due to improvement of vacuolar H + pump activity enhancing Na + and K + sequestration; overexpressing of AtVHA-C resulted in significant acidification of vacuolar pH.…”

Section: Vacuolar H + -Atpasementioning

confidence: 99%

Improving crop salt tolerance using transgenic approaches: An update and physiological analysis

Kotula

Caparrós

Zörb

et al. 2020

Plant Cell & Environment

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Salinization of land is likely to increase due to climate change with impact on agricultural production. Since most species used as crops are sensitive to salinity, improvement of salt tolerance is needed to maintain global food production. This review summarises successes and failures of transgenic approaches in improving salt tolerance in crop species. A conceptual model of coordinated physiological mechanisms in roots and shoots required for salt tolerance is presented. Transgenic plants overexpressing genes of key proteins contributing to Na + 'exclusion' (PM-ATPases with SOS1 antiporter, and HKT1 transporter) and Na + compartmentation in vacuoles (V-H + ATPase and V-H + PPase with NHX antiporter), as well as two proteins potentially involved in alleviating water deficit during salt stress (aquaporins and dehydrins), were evaluated. Of the 51 transformations, with gene(s) involved in Na + 'exclusion' or Na + vacuolar compartmentation that contained quantitative data on growth and include a non-saline control, 48 showed improvements in salt tolerance (less impact on plant mass) of transgenic plants, but with only two tested in field conditions. Of these 51 transformations, 26 involved crop species. Tissue ion concentrations were altered, but not always in the same way. Although glasshouse data are promising, field studies are required to assess crop salinity tolerance.

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Expressing Arabidopsis thaliana V-ATPase subunit C in barley (Hordeum vulgare) improves plant performance under saline condition by enabling better osmotic adjustment

Cited by 21 publications

References 65 publications

Bread Wheat With High Salinity and Sodicity Tolerance

Bread Wheat With High Salinity and Sodicity Tolerance

Cell-Based Phenotyping Reveals QTL for Membrane Potential Maintenance Associated with Hypoxia and Salinity Stress Tolerance in Barley

Improving crop salt tolerance using transgenic approaches: An update and physiological analysis

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