Expressed sequence tags of Aspergillus fumigatus: Extension of catalogue and their evaluation as putative drug targets and/or diagnostic markers

Upadhyay, Santosh Kumar; Shankar, Jata; Singh, Yogendra; Basir, Seemi Farhat; Madan, Taruna; Sarma, P. Usha

doi:10.1007/s12291-009-0024-z

Cited by 2 publications

(2 citation statements)

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“…There is a 5.9–6.9-fold increase in hsp60 mRNA expression that was observed in A. fumigatus and Aspergillus terreus at 40°C [ 13 ]. In a limited data set of expressed sequence tag analyses of A. fumigatus derived at 37°C did not observe gene encoding for Hsps [ 4 , 107 ]. Trichophyton mentagrophyte showed 4.9-fold increased expression of hsp60 when incubated at 40°C [ 13 ].…”

Section: Role Of Hsp In Stress Tolerancementioning

confidence: 99%

Role of Heat-Shock Proteins in Cellular Function and in the Biology of Fungi

Tiwari

Thakur

Shankar

2015

Biotechnology Research International

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187

130

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Stress (biotic or abiotic) is an unfavourable condition for an organism including fungus. To overcome stress, organism expresses heat-shock proteins (Hsps) or chaperons to perform biological function. Hsps are involved in various routine biological processes such as transcription, translation and posttranslational modifications, protein folding, and aggregation and disaggregation of proteins. Thus, it is important to understand holistic role of Hsps in response to stress and other biological conditions in fungi. Hsp104, Hsp70, and Hsp40 are found predominant in replication and Hsp90 is found in transcriptional and posttranscriptional process. Hsp90 and Hsp70 in combination or alone play a major role in morphogenesis and dimorphism. Heat stress in fungi expresses Hsp60, Hsp90, Hsp104, Hsp30, and Hsp10 proteins, whereas expression of Hsp12 protein was observed in response to cold stress. Hsp30, Hsp70, and Hsp90 proteins showed expression in response to pH stress. Osmotic stress is controlled by small heat-shock proteins and Hsp60. Expression of Hsp104 is observed under high pressure conditions. Out of these heat-shock proteins, Hsp90 has been predicted as a potential antifungal target due to its role in morphogenesis. Thus, current review focuses on role of Hsps in fungi during morphogenesis and various stress conditions (temperature, pH, and osmotic pressure) and in antifungal drug tolerance.

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Section: Role Of Hsp In Stress Tolerancementioning

confidence: 99%

Role of Heat-Shock Proteins in Cellular Function and in the Biology of Fungi

Tiwari

Thakur

Shankar

2015

Biotechnology Research International

Self Cite

187

130

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“…A. fumigatus is known to produce multifunctional enzymes and toxins that facilitate the adherence and hydrolysis of the components of the host cell, and complex allergens which cause severe allergic reactions. In view of the clinical importance of the allergens/antigens of Aspergillus species in the allergic and invasive disease, over 20 genes encoding A. fumigatus antigens have been recombinantly expressed and evaluated for their diagnostic importance (Table 1) [11][12][13][14][15][16][17]. So far, 34 allergens of A. fumigatus, from Asp f1 to Asp f34, have been designated by IUIS Allergen Nomenclature Committee [18].…”

Section: Interesting Biomolecules Of Aspergillimentioning

confidence: 99%

Allergens/Antigens, Toxins and Polyketides of Important Aspergillus Species

et al. 2011

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The medical, agricultural and biotechnological importance of the primitive eukaryotic microorganisms, the Fungi was recognized way back in 1920. Among various groups of fungi, the Aspergillus species are studied in great detail using advances in genomics and proteomics to unravel biological and molecular mechanisms in these fungi. Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger, Aspergillus parasiticus, Aspergillus nidulans and Aspergillus terreus are some of the important species relevant to human, agricultural and biotechnological applications. The potential of Aspergillus species to produce highly diversified complex biomolecules such as multifunctional proteins (allergens, antigens, enzymes) and polyketides is fascinating and demands greater insight into the understanding of these fungal species for application to human health. Recently a regulator gene for secondary metabolites, LaeA has been identified. Gene mining based on LaeA has facilitated new metabolites with antimicrobial activity such as emericellamides and antitumor activity such as terrequinone A from A. nidulans. Immunoproteomic approach was reported for identification of few novel allergens for A. fumigatus. In this context, the review is focused on recent developments in allergens, antigens, structural and functional diversity of the polyketide synthases that produce polyketides of pharmaceutical and biological importance. Possible antifungal drug targets for development of effective antifungal drugs and new strategies for development of molecular diagnostics are considered.

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Expressed sequence tags of Aspergillus fumigatus: Extension of catalogue and their evaluation as putative drug targets and/or diagnostic markers

Abstract: Aspergillus fumigatus a fungal pathogen is implicated in a spectrum

Cited by 2 publications

References 18 publications

Role of Heat-Shock Proteins in Cellular Function and in the Biology of Fungi

Role of Heat-Shock Proteins in Cellular Function and in the Biology of Fungi

Allergens/Antigens, Toxins and Polyketides of Important Aspergillus Species

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