Expressed Sequence Tags from <i>Phytophthora sojae</i> Reveal Genes Specific to Development and Infection

Torto-Alalibo, Trudy; Tripathy, Sucheta; Smith, Brian M.; Arredondo, Francisco; Zhou, Lecong; Li, Hua; Chibucos, Marcus C.; Qutob, Dinah; Gijzen, Mark; Mao, Chunhong; Sobral, Bruno W. S.; Waugh, Mark E.; Mitchell, Thomas K.; Dean, Ralph A.; Tyler, Brett M.

doi:10.1094/mpmi-20-7-0781

Cited by 60 publications

(66 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With few exceptions, our proteomics study agrees with or complements results from several transcriptomics studies carried out in Phytophthora (82)(83)(84)(85). Recent studies of P. sojae (83,85) and Phytophthora parasitica (82) identified multiple expressed sequence tags representing genes involved in glycolysis in mycelium or in planta.…”

Section: Candidate P Sojae Proteins Involved In Soybean Specificitysupporting

confidence: 86%

“…Recent studies of P. sojae (83,85) and Phytophthora parasitica (82) identified multiple expressed sequence tags representing genes involved in glycolysis in mycelium or in planta. Contrary to our results, differential hybridization analysis of cDNA libraries from Phytophthora nicotianae identified glycolytic genes to be upregulated in the germinating cyst (84).…”

Section: Candidate P Sojae Proteins Involved In Soybean Specificitymentioning

confidence: 99%

See 1 more Smart Citation

Cross-species Global Proteomics Reveals Conserved and Unique Processes in Phytophthora sojae and Phytophthora ramorum

Savidor

Donahoo

Hurtado‐Gonzales

et al. 2008

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

Phytophthora ramorum and Phytophthora sojae are destructive plant pathogens. P. sojae has a narrow host range, whereas P. ramorum has a wide host range. A global proteomics comparison of the vegetative (mycelium) and infective (germinating cyst) life stages of P. sojae and P. ramorum was conducted to identify candidate proteins involved in host range, early infection, and vegetative growth. Sixty-two candidates for early infection, 26 candidates for vegetative growth, and numerous proteins that may be involved in defining host specificity were identified. In addition, common life stage proteomic trends between the organisms were observed. In mycelia, proteins involved in transport and metabolism of amino acids, carbohydrates, and other small molecules were up-regulated. In the germinating cysts, up-regulated proteins associated with lipid transport and metabolism, cytoskeleton, and protein synthesis were observed. It appears that the germinating cyst catabolizes lipid reserves through the ␤-oxidation pathway to drive the extensive protein synthesis necessary to produce the germ tube and initiate infection. Once inside the host, the pathogen switches to vegetative growth in which energy is derived from glycolysis and utilized for synthesis of amino acids and other molecules that assist survival in the plant tissue. Molecular & Cellular Proteomics 7:1501-1516, 2008.

show abstract

Section: Candidate P Sojae Proteins Involved In Soybean Specificitysupporting

confidence: 86%

Section: Candidate P Sojae Proteins Involved In Soybean Specificitymentioning

confidence: 99%

Cross-species Global Proteomics Reveals Conserved and Unique Processes in Phytophthora sojae and Phytophthora ramorum

Savidor

Donahoo

Hurtado‐Gonzales

et al. 2008

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

show abstract

“…Additionally, the ␤-(1, 3)-endoglucanases are cell wall-associated in P. ramorum, suggesting an important role for this gene family in the interaction with the host plants (32). Finally, in P. sojae, these genes were found to be specifically expressed during infection (33).…”

Section: Resultsmentioning

confidence: 98%

“…2, families 3403 and 990), glutaredoxin (family 3430), and the catalase-peroxidase (family 220). These families were classified as putative pathogenicity genes by TortoAlalibo and colleagues (33). Finally, the expansion of two other gene families can be explained by their role in the process of pathogenesis: first, the glucokinases (family 2414) that are expressed during the first 6 h of interaction with its host Glycine max (34), and second, the lipases (family 845), which are also shown to be potentially infection related (11).…”

Section: Resultsmentioning

confidence: 99%

Whole-genome analysis reveals molecular innovations and evolutionary transitions in chromalveolate species

Martens

Vandepoele

Peer

2008

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The chromalveolates form a highly diverse and fascinating assemblage of organisms, ranging from obligatory parasites such as Plasmodium to free-living ciliates and algae such as kelps, diatoms, and dinoflagellates. Many of the species in this monophyletic grouping are of major medical, ecological, and economical importance. Nevertheless, their genome evolution is much less well studied than that of higher plants, animals, or fungi. In the current study, we have analyzed and compared 12 chromalveolate species for which wholesequence information is available and provide a detailed picture on gene loss and gene gain in the different lineages. As expected, many gene loss and gain events can be directly correlated with the lifestyle and specific adaptations of the organisms studied. For instance, in the obligate intracellular Apicomplexa we observed massive loss of genes that play a role in general basic processes such as amino acid, carbohydrate, and lipid metabolism, reflecting the transition of a free-living to an obligate intracellular lifestyle. In contrast, many gene families show species-specific expansions, such as those in the plant pathogen oomycete Phytophthora that are involved in degrading the plant cell wall polysaccharides to facilitate the pathogen invasion process. In general, chromalveolates show a tremendous difference in genome structure and evolution and in the number of genes they have lost or gained either through duplication or horizontal gene transfer.gene gain ͉ gene loss

show abstract

“…The AeCHS1 and AeCHS2 sequences were used as probes to perform BLASTp similarity searches (1) against the nonredundant protein database at the National Center for Biotechnology (NCBI) (http://www.ncbi.nlm.nih.gov/) and tBLASTn similarity searches against the Phytophthora genome project databases at the U.S. Department of Energy (DOE) Joint Genome Institute (http://www.jgi.doe.gov/) and the Broad Institute (http://www.broad.mit.edu/). The identified Phytophthora sojae and P. infestans CHS sequences corresponded to ESTs found at the Oomycete Genomics Database (OGD) (http://www.oomycete.org/ogd/), Phytophthora Functional Genomics Database (PFGD) (http://www.pfgd.org/), or Phytophthora Soybean EST database (PhESTDB 1.0) (http://phytophthora.vbi.vt.edu/EST/) (31,80). A series of consensus sequences for the aligned oomycete CHSs, spanning from 50 to 90% similarity, was built using the software Consensus (http://www.bork.embl .de/).…”

mentioning

confidence: 91%

Cell Wall Chitosaccharides Are Essential Components and Exposed Patterns of the Phytopathogenic OomyceteAphanomyces euteiches

et al. 2008

View full text Add to dashboard Cite

Chitin is an essential component of fungal cell walls, where it forms a crystalline scaffold, and chitooligosaccharides derived from it are signaling molecules recognized by the hosts of pathogenic fungi. Oomycetes are cellulosic fungus-like microorganisms which most often lack chitin in their cell walls. Here we present the first study of the cell wall of the oomycete Aphanomyces euteiches, a major parasite of legume plants. Biochemical analyses demonstrated the presence of ca. 10% N-acetyl-D-glucosamine (GlcNAc) in the cell wall. Further characterization of the GlcNAc-containing material revealed that it corresponds to noncrystalline chitosaccharides associated with glucans, rather than to chitin per se. Two putative chitin synthase (CHS) genes were identified by data mining of an A. euteiches expressed sequence tag collection and Southern blot analysis, and full-length cDNA sequences of both genes were obtained. Phylogeny analysis indicated that oomycete CHS diversification occurred before the divergence of the major oomycete lineages. Remarkably, lectin labeling showed that the Aphanomyces euteiches chitosaccharides are exposed at the cell wall surface, and study of the effect of the CHS inhibitor nikkomycin Z demonstrated that they are involved in cell wall function. These data open new perspectives for the development of antioomycete drugs and further studies of the molecular mechanisms involved in the recognition of pathogenic oomycetes by the host plants.

show abstract

Expressed Sequence Tags from Phytophthora sojae Reveal Genes Specific to Development and Infection

Cited by 60 publications

References 64 publications

Cross-species Global Proteomics Reveals Conserved and Unique Processes in Phytophthora sojae and Phytophthora ramorum

Cross-species Global Proteomics Reveals Conserved and Unique Processes in Phytophthora sojae and Phytophthora ramorum

Whole-genome analysis reveals molecular innovations and evolutionary transitions in chromalveolate species

Cell Wall Chitosaccharides Are Essential Components and Exposed Patterns of the Phytopathogenic OomyceteAphanomyces euteiches

Contact Info

Product

Resources

About