Expressed Sequence Tag Analysis of<i>Physa acuta</i>: A Freshwater Pulmonate in Korea

Lee, Yong Seok; Lee, Seung-Gyu; Kang, Se Won; Jeong, Ji Eun; Baek, Moon Ki; Choi, Sang-Haeng; Chae, Soo-Cheon; Jo, Yong Hun; Han, Yeon Soo; Park, Hong-Seog

doi:10.2983/035.030.0119

Cited by 7 publications

(3 citation statements)

References 50 publications

(23 reference statements)

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“…Complete mt genomes were characterized from single individual snails, one each from P. acuta isolates A and B. PCR primers (Table 1 ) were designed and optimized using Primer3 ( Rozen & Skaletsky, 2000 ) to target conserved regions of mt genes that were identified in alignments of previously reported complete mt genome sequences from panpulmonate species and EST data available from GenBank ( Lee et al , 2011 ; White et al , 2011 ). High fidelity, long distance (LD)-PCR (Advantage Genomic LA Polymerase Mix, Clontech) was used to generate overlapping amplicons that encompassed the complete mt genome.…”

Section: Methodsmentioning

confidence: 99%

“…Physella acuta ( Draparnaud, 1805 ), frequently designated Physa acuta , is a widely used model snail that is widely distributed, readily obtainable and can be maintained with ease in the laboratory. This species serves as an aquatic biomarker due to its ability to live in polluted water ( Sánchez-Argüello, Fernández & Tarazona, 2009 ; Lee et al , 2011 ), it has high salinity thresholds ( Kefford & Nugegoda, 2005 ) and it is used in population and mating studies ( Bousset et al , 2004 ; Dillon, Wethington & Lydeard, 2011 ). As an invasive species, P. acuta has been studied for competitiveness with indigenous gastropod fauna ( Madsen & Frandsen, 1989 ; Albrecht et al , 2009 ).…”

Section: Introductionmentioning

confidence: 99%

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Physella acuta: atypical mitochondrial gene order among panpulmonates (Gastropoda)

Nolan

Bergthorsson

Adema

2014

Journal of Molluscan Studies

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Mitochondrial (mt) sequences are frequently used for phylogenetic reconstruction and for identification of species of molluscs. This study expands the phylogenetic range of Hygrophila (Panpulmonata) for which such sequence data are available by characterizing the full mt genome of the invasive freshwater snail Physella acuta (Physidae). The mt genome sequences of two P. acuta isolates from Stubblefield Lake, New Mexico, USA, differed in length (14,490 vs 14,314 bp) and showed 11.49% sequence divergence, whereas ITS1 and ITS2 sequences from the nuclear genome differed by 1.75%. The mt gene order of P. acuta (cox1, P, nad6, nad5, nad1, D, F, cox2, Y, W, nad4L, C, Q, atp6, R, E, rrnS, M, T, cox3, I, nad2, K, V, rrnL, L1, A, cytb, G, H, L2, atp8, N, nad2, S1, S2, nad4) differs considerably from the relatively conserved gene order within Panpulmonata. Phylogenetic trees show that the 13 protein-encoding mt gene sequences (equivalent codons) of P. acuta group according to gastropod phylogeny, yet branch lengths and dN/dS ratios for P. acuta indicate elevated amino acid substitutions relative to other gastropods. This study indicates that mt sequences of P. acuta are phylogenetically informative despite a considerable intraspecific divergence and the atypical gene order in its mt genome.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Physella acuta: atypical mitochondrial gene order among panpulmonates (Gastropoda)

Nolan

Bergthorsson

Adema

2014

Journal of Molluscan Studies

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“…Die feit dat waterbronne as stortplek gebruik word vir 'n verskeidenheid van antropogeniese afvalstowwe en dat varswatermolluske permanente waterbewoners is, wat oor beperkte voortbewegingsvermoë beskik, kan moontlik tot bogenoemde statistieke bydra. Varswatermolluske beskik egter in die algemeen, volgens die literatuur, oor 'n relafief wye verdraagsaamheid teenoor faktore soos pH (Boycott 1936;Deschiens 1956;Malek 1958;Brown 1994) en geleidingsvermoë (Schutte & Frank 1964;Jennings 1976;Brown 1994); terwyl genetiese biomerkers by sommige spesies geïdentifiseer is wat hulle in staat stel om stres, veroorsaak deur 'n verskeidenheid van besoedelstowwe, te verwerk (Lee et al 2011). Verder is.…”

unclassified

Short-term threats for the sustained survival of freshwater Mollusca in the Olifants River and selected tributaries.

Kock

Wolmarans

Kemp

et al. 2013

Suid-Afrikaanse Tydskr Natuurwetenskap Tegnol

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The conservation status of less than 2% of the more or less 7000 mollusk species known worldwide have been properly assessed. Consequently the general level of imperilment is poorly documented and almost certainly underestimated. Freshwater mollusks live permanently in water, have limited means of movement and are exposed to a variety of anthropogenic waste products due to the fact that waterbodies often act as sinks for a large array of harmful pollutants. The Olifants River is often described as one of the most polluted rivers in Southern Africa and is progressively subjected to extremely high pressure with regard to natural resources and associated rural transformation and pollution. Little is on record regarding the diversity of the Mollusca in the Olifants River; therefore, in the present study, four surveys of the molluscs were conducted in this river and selected tributaries during two consecutive years at three localities situated on the Highveld and four localities situated in the Lowveld respectively. The pH and electric conductivity of the water were determined during each survey at each one of the localities and values ranged from 6.93 to 9.50, and 110 µS to 1336 µS, for pH and conductivity respectively. A total of 25 mollusk species were collected during the four surveys which included the exotic invader species Lymnaea columella, Physa acuta, Aplexa marmorata and Tarebia granifera. The latter species yielded the highest number of specimens by far, mainly at a locality which could be described as largely transformed. The results of this investigation can serve as a point of departure for future surveys to evaluate the impact of anthropogenic disturbances on the mollusc diversity in the Olifants River and catchment.

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Characterization of Physa acuta expressed sequence tags and transcript mining following cadmium exposure

et al. 2015

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Expressed Sequence Tag Analysis ofPhysa acuta: A Freshwater Pulmonate in Korea

Cited by 7 publications

References 50 publications

Physella acuta: atypical mitochondrial gene order among panpulmonates (Gastropoda)

Physella acuta: atypical mitochondrial gene order among panpulmonates (Gastropoda)

Short-term threats for the sustained survival of freshwater Mollusca in the Olifants River and selected tributaries.

Characterization of Physa acuta expressed sequence tags and transcript mining following cadmium exposure

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