Expresión génica del factor de crecimiento BMP15, GDF9, FGF2 y sus receptores en células foliculares bovinas

Abstract

Introduction. Growth and follicular maturation involve transformations of various components of the follicle, such as the oocyte, granulosa and techa cells. Several growth factors, including differentiation growth factor 9 (GDF9), bone morphogenic protein 15 (BMP15) and basic fibroblast growth factor (FGF2) are important for follicular development and oocyte maturation, by its ability to increase the proliferation of granulosa, techa cells and the ovarian stroma. Objetive. Evaluate mRNA expression of GDF9, BMP15, FGF2 and their main receptors, transforming growth factor beta receptor 1 (TGFβ-R1), bone morphogenetic protein receptor, type IB (BMPR-IB) and fibroblast growth factor receptor 2 (FGFR2) in bovine follicular cells. Materials and methods. Total RNA was isolated from pooled samples of oocytes (OOs), cumulus cells (CCs) of cumulus oocyte complexes (COCs) and follicular cell pellets (PCs) of 70 ovaries obtained from 96 beef heifers, collected at a local abattoir. The expression pattern of growth factors and their receptors in follicular bovine cells was evaluated by reverse transcriptase polymerase chain reaction (RT-PCR). Results. The mRNA transcripts encoding GDF9, BMP15, FGF2, TGFβ-R1, BMPR-IB and FGFR2 genes were detected, by RT-PCR, in all studied cells. This is the first time that the expression of TGFβ-R1 and BMPR-IB receptors is reported in bovine oocytes. Conclusions. The presence of growth factors and receptor transcripts in the studied cells indicate that these factors could act as paracrine and autocrine regulators of folliculogenesis.

Epidermal Growth Factor (EGF) and Basic Fibroblast Growth Factor (FGF2) Profiles in Follicular Fluid, Maturation Media and Embryo Culture of Bali Cattle

“…Known ECM is different during in vitro maturation and in vivo (Dunning et al, 2012). Research by Reineri et al (2018) on the evaluation of mRNA expression from bone morphogenetic protein 15 (BMP15), Growth factor and differentiation factor 9 (GDF9), FGF2 and its main receptors, altering growth factor 1 beta receptor (TGFβ-R1), bone morphogenetic protein receptor, IB type (BMPR-IB) and fibroblast growth factor receptor 2 (FGFR2) in bovine follicle cells, found in oocyte cumulus and follicle cells in cattle.…”

Follicular wave dynamics and Growth factors gene expression in Braford heifers

“…The growth differentiation factor 9 (GDF9), the bone morphogenetic protein 15 (BMP15) and the basic fibroblast growth factor (FGF2), with their main receptors, have been involved in dominant follicle selection, regulating important events such as proliferation of granulosa cells, differentiation and steroidogenesis (Chang, Qiao, & Leung, 2016; Mishra et al., 2016). Furthermore, the presence of GDF9, BMP15, FGF2, transforming growth factor beta receptor 1 (TGFβ‐R1), bone morphogenetic protein receptor type IB (BMPR‐IB) and fibroblast growth factor receptor 2 (FGFR2) transcripts in the follicular cells suggest that these factors could act as paracrine and autocrine regulators in folliculogenesis (Reineri et al., 2018). Many studies correlating cumulus gene expression of growth factors with different outcome measures such as oocyte maturity, fertilization, embryo development, implantation and pregnancy have been performed (Li et al., 2014, 2015; Mckenzie et al., 2004; Zhang, Jafari, Barnes, & Confino, 2005).…”

“…Furthermore, the presence of GDF9, BMP15, FGF2, transforming growth factor beta receptor 1 (TGFβ-R1), bone morphogenetic protein receptor type IB (BMPR-IB) and fibroblast growth factor receptor 2 (FGFR2) transcripts in the follicular cells suggest that these factors could act as paracrine and autocrine regulators in folliculogenesis (Reineri et al, 2018). Many studies correlating cumulus gene expression of growth factors with different outcome measures such as oocyte maturity, fertilization, embryo development, implantation and pregnancy have been performed (Li et al, 2014(Li et al, , 2015Mckenzie et al, 2004;Zhang, Jafari, Barnes, & Confino, 2005).…”