Exposure to waterpipe smoke induces renal functional and oxidative biomarkers variations in mice

Rababa’h, Abeer; Sultan, Bilal B.; Alzoubi, Karem H.; Khabour, Omar F.; Ababneh, Mera A.

doi:10.1080/08958378.2016.1210703

Cited by 21 publications

(17 citation statements)

References 23 publications

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“…We found that C57BL/6J strain was more susceptible to acute WPS-induced oxidative stress response among the two strains. Our results are consistent with earlier findings that WPS induces oxidative stress by generation of ROS and depletion in GSH levels and increase in GSSG levels in several organs (Nemmar et al 2013;Ali et al 2017;Alzoubi et al 2015;Rababa'h et al 2016). It could also be possible due to the formation of GSH conjugates or rapid consumption of GSH during the breakdown of free radicals induced by WPS, thereby altering GSH redox system (Rahman and MacNee 1999).…”

Section: Discussionsupporting

confidence: 94%

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Strain- and sex-dependent pulmonary toxicity of waterpipe smoke in mouse

2018

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Waterpipe smoking is emerging as a form of tobacco smoking, but its lung health/risks is not known. It has been shown that different mouse strains show differences in susceptibility to tobacco smoke. However, the effect of waterpipe smoke (WPS) exposure and strain differences in susceptibility to oxidative and inflammatory responses is not known. Here, we showed acute WPS exposure induced oxidative stress and inflammatory response in C57BL/6J and BALB/cJ mouse strains. WPS exposure induced inflammatory cell influx (neutrophils and T‐lymphocytes) in bronchoalveolar lavage fluid (BAL fluid), which varied among mouse strains. Proinflammatory cytokines release differed among both the strains, but was significantly increased in C57BL/6J mice. Myeloperoxidase levels in BAL fluid were increased significantly in both the strains. Total reduced glutathione (GSH) level was decreased, whereas the level of oxidized or glutathione disulfide (GSSG) increased in lungs of both the strains. Similarly, the level of lipid peroxidation markers, 15‐isoprostane (plasma), malondialdehyde and 4‐hydroxy‐2‐nonenal (lung homogenates) were increased by WPS. Our data suggest that, oxidative stress and inflammatory responses are influenced by strain characteristics during acute WPS exposure. Overall, C57BL/6J mice showed more susceptibility to oxidative stress and inflammatory responses compared to BALB/cJ mice. Acute WPS mediated pulmonary toxicity is differentially regulated in different mouse strains.

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Section: Discussionsupporting

confidence: 94%

“…; Rababa'h et al. ). It could also be possible due to the formation of GSH conjugates or rapid consumption of GSH during the breakdown of free radicals induced by WPS, thereby altering GSH redox system (Rahman and MacNee ).…”

Section: Discussionmentioning

confidence: 97%

Strain- and sex-dependent pulmonary toxicity of waterpipe smoke in mouse

2018

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“…Together, these SOD enzymes scavenge superoxide anion into molecular oxygen and hydrogen peroxide, then CAT and GPx reduce hydrogen peroxide, thus preventing production of highly toxic hydroxyl radical [64]. Growing evidence indicates that cigarette smoking or CSE significantly decrease the activities of anti-oxidative enzymes both in vitro and in vivo researches [65,66]. In the present study, our results displayed a significant reduction in activities of SOD1, SOD2, CAT and total GPx in CSE rat placentas, however, the decreased activities of the anti-oxidative enzymes were improved with NaHS application.…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Hydrogen Sulfide Against Cigarette Smoke Exposure-Induced Placental Oxidative Damage by Alleviating Redox Imbalance via Nrf2 Pathway in Rats

Zhao¹,

Fang²,

Yan³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Cigarette smoke exposure (CSE) during pregnancy is a well-recognized health hazard that causes placental damage. Hydrogen sulfide (H₂S) has been reported to protect multiple organs from injury. However, the protective effects of H₂S have not been tested in the placenta. This study aimed to explore the potential of H₂S in protecting placenta against oxidative injury induced by CSE during pregnancy and the possible underlying mechanisms. Methods: Pregnant SD rats were randomly divided into 4 groups: NaCl, NaHS (a donor of H₂S), CSE and CSE+NaHS. Placental oxidative damage was detected by 8-hydroxy-2-deoxyguanosine (8-OHdG) stain and malondialdehyde (MDA) assay. Placental redox status was assessed by measuring reactive oxygen species (ROS), total antioxidant capacity (T-AOC) and glutathione (GSH) levels, as well as copper/zinc SOD (SOD1), manganese SOD (SOD2), catalase (CAT) and glutathione peroxidase (GPx) activities and expressions. Meanwhile, nuclear factor erythroid 2-related factor 2 (Nrf2) was analyzed by immunohistochemistry, real-time PCR and Western blot. Results: We found that NaHS markedly reduced the elevated levels of 8-OHdG and MDA induced by CSE. Further, NaHS treatment effectively mitigated CSE-induced placental redox imbalance by inhibiting ROS production, restoring T-AOC level, increasing GSH/GSSG ratio, and augmenting SOD1 SOD2, CAT and GPx activities and expressions. More notably, NaHS administration also reversed the aberrant decrease of Nrf2 due to CSE in rat placentas. Conclusion: Our data demonstrate that H₂S can protect against CSE-induced placental oxidative damage probably by alleviating redox imbalance via Nrf2 pathway.

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“…While some studies have reported negative health effects of CS on the kidney (Xia et al, 2017), little is known about the potential adverse impact of WPS exposure on the kidney. It has been recently reported that exposure to WPS causes oxidative stress in the kidney (Rababa'h et al, 2016). However, the mechanisms underlying this effect remain unknown.…”

Section: Introductionmentioning

confidence: 99%

Nose-Only Water-Pipe Smoke Exposure in Mice Elicits Renal Histopathological Alterations, Inflammation, Oxidative Stress, DNA Damage, and Apoptosis

et al. 2020

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The prevalence of water-pipe tobacco smoking is increasing worldwide, and is relatively high among youth and young adults. Exposure to water-pipe smoke (WPS) has been reported to affect various systems including the respiratory, cardiovascular and reproductive systems. However, the impact of WPS exposure on the kidney has received only scant attention. Here, we assessed the effect of nose-only WPS exposure for one or four consecutive weeks on renal histology, inflammation, oxidative stress, DNA damage, and apoptosis. The duration of the session was 30 min/day and 5 days/week. Control mice were exposed to air. Light and electron microcopy analysis revealed that the WPS exposure (especially at 4-week time point) caused degeneration of the endothelial cells of the glomerular capillaries and vacuolar degenerations of the proximal convoluted tubules. WPS exposure also significantly decreased the creatinine clearance, and significantly increased proteinuria and urinary kidney injury molecule-1 (KIM-1) concentration. Kidney lipid peroxidation, reactive oxygen species, and oxidized glutathione were significantly increased. WPS exposure also affected the concentration of reduced glutathione and the activity of catalase. Likewise, renal concentrations of interleukin (IL)-6, IL-1β and KIM-1 were augmented by WPS exposure. Moreover, WPS caused DNA damage as evaluated by comet assay, and increased the expression of cleaved caspase-3 and cytochrome C in the kidney. We conclude that exposure of mice to WPS caused renal histopathological alterations, inflammation, oxidative stress, DNA damage, and apoptosis. If the latter findings could be substantiated by controlled human studies, it would be an additional cause for disquiet about an established public health concern.

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Exposure to waterpipe smoke induces renal functional and oxidative biomarkers variations in mice

Abstract: In conclusion, chronic waterpipe smoke affects the kidney parameter and antioxidant markers, therefore affecting its functionality of detoxifying and removal of poisonous material from the body.

Cited by 21 publications

References 23 publications

Strain- and sex-dependent pulmonary toxicity of waterpipe smoke in mouse

Strain- and sex-dependent pulmonary toxicity of waterpipe smoke in mouse

Protective Effects of Hydrogen Sulfide Against Cigarette Smoke Exposure-Induced Placental Oxidative Damage by Alleviating Redox Imbalance via Nrf2 Pathway in Rats

Nose-Only Water-Pipe Smoke Exposure in Mice Elicits Renal Histopathological Alterations, Inflammation, Oxidative Stress, DNA Damage, and Apoptosis

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