Exposure to Vinyl Chloride and Its Influence on Western Diet-Induced Cardiac Remodeling

Liang, Yaqin; Lang, Anna L.; Zhang, Jian; Chen, Jing; Wang, Kai; Chen, Liya; Beier, Juliane I.; Yan, Qian; Cai, Lu

doi:10.1021/acs.chemrestox.8b00043

Cited by 12 publications

(18 citation statements)

References 47 publications

(101 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All mice underwent transthoracic echocardiography (echo) for assessment of cardiac function, as described elsewhere 25. Briefly, a high-resolution imaging system (Vevo 770, Visual Sonics, Canada) equipped with a high-frequency RMV 707B ultrasound probe was used to examine isoflurane anesthetized mice.…”

Section: Methodsmentioning

confidence: 99%

Protective Effect of Lactobacillus rhamnosus GG and its Supernatant against Myocardial Dysfunction in Obese Mice Exposed to Intermittent Hypoxia is Associated with the Activation of Nrf2 Pathway

Wang

Cai

et al. 2019

Int. J. Biol. Sci.

Self Cite

View full text Add to dashboard Cite

Prolonged intermittent hypoxia (IH) has been shown to impair myocardial function (mainly via oxidative stress and inflammation) and modify gut microbiota in mice. Gut microbiota plays an important role in health and disease, including obesity and cardiovascular disease (CVD). Probiotics refer to live microorganisms that confer health benefits on the host after administration in adequate amounts. Research on novel probiotics related therapies has evoked much attention. In our previous study, both Lactobacillus rhamnosus GG (LGG) and LGG cell-free supernatant (LGGs) were found to protect against alcohol-induced liver injury and steatosis; however, the effects of LGG and LGGs on cardiac tissues of obese mice exposed to IH have not been determined. Here we exposed high-fat high-fructose diet (HFHFD)-induced obese mice to IH, to establish a model of obesity with obstructive sleep apnea (OSA). Mice were divided into four groups: (1) HFHFD for 15 weeks; (2) HFHFD for 15 weeks with IH in the last 12 weeks (HFHFD/IH); (3) and (4) HFHFD/IH plus oral administration of either LGG (10 9 CFU bacteria/day) or LGGs (dose equivalent to 10 9 CFU bacteria/day) over the 15 weeks, respectively. Compared to HFHFD mice, HFHFD/IH-mice showed heart dysfunction with significant cardiac remodeling and inflammation; all these pathological and functional alterations were prevented by treatment with both LGG and LGGs (no significant difference between LGG and LGGs in this respect). The cardioprotective effect of LGG and LGGs against IH/HFHFD was associated with up-regulation of nuclear factor erythroid 2-related factor 2(Nrf2)-mediated antioxidant pathways. Our findings suggest a cardioprotective effect of LGG and LGGs in obese mice with OSA.

show abstract

Section: Methodsmentioning

confidence: 99%

Protective Effect of Lactobacillus rhamnosus GG and its Supernatant against Myocardial Dysfunction in Obese Mice Exposed to Intermittent Hypoxia is Associated with the Activation of Nrf2 Pathway

Wang

Cai

et al. 2019

Int. J. Biol. Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although the protocol presented here is a software-driven approach, our experience has shown that the manual approach is also an accurate and consistent method of exposure 12,24 . Moreover, it is easily applicable to multiple research areas including other organ damage 23 caused by volatile organic compound exposure 22 . Notably, this model may more closely resemble the pathogenesis of human co-exposures to environmental chemicals and underlying disease 5 .…”

Section: Representative Resultsmentioning

confidence: 99%

“…This model of toxicant-induced liver injury can be used for other volatile organic compounds and also to study other interactions that may impact the liver and other organ systems 8,22,23 . Moreover, this model has been, and can be further, used to investigate intervention therapies and indepth mechanistic studies of the mode of action for this prevalent toxicant 24 .…”

Section: Representative Resultsmentioning

confidence: 99%

“…NOTE: There are multiple inhalation exposure systems commercially available, ranging from 'nose-only' to 'whole-body' exposure and manual to automated systems. Data previously published by this group was derived from a whole-body manual system 12,23,24 . A diagram describing the automated inhalation exposure system is shown in Figure 2.…”

Section: Vinyl Chloride Inhalation Exposure Systemmentioning

confidence: 99%

“…The length of time and concentration of compound administered can be varied. Although initially developed for determination of liver injury, other organ systems can and have been studied with this model 23 . Investigators who aim to study chronic exposures with animals, but wish to minimize animal stress, should consider using this model.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Vinyl Chloride and High-Fat Diet as a Model of Environment and Obesity Interaction

Lang

Goldsmith

Schnegelberger

et al. 2020

JoVE

Self Cite

View full text Add to dashboard Cite

Vinyl chloride (VC), an abundant environmental contaminant, causes steatohepatitis at high levels, but is considered safe at lower levels.Although several studies have investigated the role of VC as a direct hepatotoxicant, the concept that VC modifies sensitivity of the liver to other factors, such as nonalcoholic fatty liver disease (NAFLD) caused by high-fat diet (HFD) is novel. This protocol describes an exposure paradigm to evaluate the effects of chronic, low-level exposure to VC. Mice are acclimated to low-fat or high-fat diet one week prior to the beginning of the inhalation exposure and remain on these diets throughout the experiment. Mice are exposed to VC (sub-OSHA level: <1 ppm) or room air in inhalation chambers for 6 hours/day, 5 days/week, for up to 12 weeks. Animals are monitored weekly for body weight gain and food consumption. This model of VC exposure causes no overt liver injury with VC inhalation alone. However, the combination of VC and HFD significantly enhances liver disease. A technical advantage of this co-exposure model is the whole-body exposure, without restraint. Moreover, the conditions more closely resemble a very common human situation of a combined exposure to VC with underlying nonalcoholic fatty liver disease and therefore support the novel hypothesis that VC is an environmental risk factor for the development of liver damage as a complication of obesity (i.e., NAFLD). This work challenges the paradigm that the current exposure limits of VC (occupational and environmental) are safe. The use of this model can shed new light and concern on the risks of VC exposure. This model of toxicant-induced liver injury can be used for other volatile organic compounds and to study other interactions that may impact the liver and other organ systems.

show abstract

Mechanism of subchronic vinyl chloride exposure combined with a high‐fat diet on hepatic steatosis

Jin

Chen

Pan

et al. 2021

J of Applied Toxicology

View full text Add to dashboard Cite

Vinyl chloride (VC) is a common industrial organic chlorine and environmental pollutant. In recent years, the dietary structure of residents especially Chinese has gradually shifted to western dietary patterns. VC aggravates dietary fatty acid‐induced hepatic steatosis, but its mechanism is still unclear. And if the risk factors for steatosis persist, more severe diseases such as fibrosis and cirrhosis will occur. Therefore, we studied the effects and mechanisms of VC (160 and 800 mg/m3) and its metabolite (chloroacetaldehyde, 2.25, 4.5, and 9 μM) on hepatic steatosis of high‐fat diet (HFD)‐fed mice and palmitic acid (PA, 100 μM) treated HepG2 cells. Liver and serum biochemical indicators and pathological staining of the liver showed that the hepatic steatosis of VC combined with HFD groups was more severe than that of single‐exposure groups (HFD group, low‐dose VC group, and high‐dose VC group). Moreover, VC enhanced HFD‐induced oxidative stress (OS) and endoplasmic reticulum stress (ERS) and further upregulated the expression of sterol regulatory element‐binding protein 1 (SREBP‐1) and FAS. Besides, antioxidants and ERS inhibitors reduced the steatosis of HepG2 cells induced by VC metabolites and PA. These results suggest that VC exposure can enhance the degree of hepatic steatosis in HFD‐fed mice. VC combined with HFD led to OS and ERS and upregulated the expression of de novo lipogenesis‐related proteins, which may be related to the occurrence of hepatic steatosis. And the increased expression of CYP2E1 induced by VC combined with HFD may be the cause of OS.

show abstract

Exposure to Vinyl Chloride and Its Influence on Western Diet-Induced Cardiac Remodeling

Cited by 12 publications

References 47 publications

Protective Effect of Lactobacillus rhamnosus GG and its Supernatant against Myocardial Dysfunction in Obese Mice Exposed to Intermittent Hypoxia is Associated with the Activation of Nrf2 Pathway

Protective Effect of Lactobacillus rhamnosus GG and its Supernatant against Myocardial Dysfunction in Obese Mice Exposed to Intermittent Hypoxia is Associated with the Activation of Nrf2 Pathway

Vinyl Chloride and High-Fat Diet as a Model of Environment and Obesity Interaction

Mechanism of subchronic vinyl chloride exposure combined with a high‐fat diet on hepatic steatosis

Contact Info

Product

Resources

About