Aims
To understand the species composition, the relative content, and diversity of nitrogen fixing bacteria in Cenchrus fungigraminus rhizophere, and to screen nitrogen-fixing bacteria to study their potential role in plant growth promotion.
Methods
Soil samples were collected from 4 depth (G1, G2, G3 and G4) of C. fungigraminus rhizophere and physical and chemical properties were determined. The diversity and abundance of nitrogen-fixing bacteria and nifH gene copy were analyzed. Nitrogen-fixing bacteria were screened and selected for studying the C. fungigraminus seedlings growth promotion.
Results
The highest diversity and abundance of nitrogen-fixing bacteria were observed in C. fungigraminus rhizosphere (G2), which mainly included Proteobacteria (93.91%), Actinobacteria (0.42%), Firmicutes (0.18%) and significantly effected by total nitrogen, available nitrogen and depth. The nifH gene copy was also highest (1.56 ± 0.17×107 copies/g) in G2. Rhizobium pusense NO.8 and NO.28 were isolated in G1 and G2, respectively, with nitrogenase activity of 1.45 ± 0.04 and 2.00 ± 0.07 U/g. The promotion experiment revealed that plant height, root length, leaf length of C. fungigramminus seedlings treated with both strains were significantly increased 56.79%, 76.99% and 55.71%, and significantly increased on moisture and total nitrogen of planting soil compare with control (P < 0.05). The available nitrogen, organic matter and organic carbon in soil with R. pusense NO.28 have significantly increased 3.09, 5.77 and 5.77 times.
Conclusion
Nitrogen-fixing bacteria in C. fungigraminus rhizosphere soil is rich and play a significant role in promoting its seedlings growth. The study provides the development of PGPRs from C. fungigraminus rhizosphere to be biological fertilizers.