Exposure to Ti4Al4V Titanium Alloy Leads to Redox Abnormalities, Oxidative Stress, and Oxidative Damage in Patients Treated for Mandible Fractures

Abstract

Due to the high biotolerance, favourable mechanical properties, and osseointegration ability, titanium is the basic biomaterial used in maxillofacial surgery. The passive layer of titanium dioxide on the surface of the implant effectively provides anticorrosive properties, but it can be damaged, resulting in the release of titanium ions to the surrounding tissues. The aim of our work was to evaluate the influence of Ti6Al4V titanium alloy on redox balance and oxidative damage in the periosteum surrounding the titanium miniplates and screws as well as in plasma and erythrocytes of patients with mandibular fractures. The study included 31 previously implanted patients (aged 21–29) treated for mandibular fractures and 31 healthy controls. We have demonstrated increased activity/concentration of antioxidants both in the mandibular periosteum and plasma/erythrocytes of patients with titanium mandibular fixations. However, increased concentrations of the products of oxidative protein and lipid modifications were only observed in the periosteum of the study group patients. The correlation between the products of oxidative modification of the mandible and antioxidants in plasma/erythrocytes suggests a relationship between the increase of oxidative damage at the implantation site and central redox disorders in patients with titanium miniplates and screws.

Redox Balance, Antioxidant Defense, and Oxidative Damage in the Hypothalamus and Cerebral Cortex of Rats with High Fat Diet-Induced Insulin Resistance

“…However, membrane lipids are the most exposed to OH • and other ROS [ 34 ]. Cellular lipids are also a rich source of polyunsaturated fatty acids (PUFAs) that are particularly susceptible to oxidation via oxidative stress [ 39 , 48 ]. Therefore, this is not surprising that the hypothalamic oxidative damage has only been reported to the lipid molecules.…”

Salivary Antioxidants and Oxidative Stress in Psoriatic Patients: Can Salivary Total Oxidant Status and Oxidative Status Index Be a Plaque Psoriasis Biomarker?

“…Under laboratory conditions, the samples were centrifuged in order to separate the plasma from erythrocytes (1500 × g; 4°C, 10 minutes). Erythrocytes were rinsed three times with 0.9% cold NaCl (v/v) and then haemolysed by adding 50 mM cold phosphate buffer (pH 7.4) 1 : 9 (v/v) [28]. To prevent sample oxidation, 0.5 M BHT (Sigma-Aldrich, Saint Louis, MO, USA; 10 μL/mL blood) was added to the blood [28].…”

“…Erythrocytes were rinsed three times with 0.9% cold NaCl (v/v) and then haemolysed by adding 50 mM cold phosphate buffer (pH 7.4) 1 : 9 (v/v) [28]. To prevent sample oxidation, 0.5 M BHT (Sigma-Aldrich, Saint Louis, MO, USA; 10 μL/mL blood) was added to the blood [28]. The samples with signs of undesired haemolysis were excluded from the study.…”

Salivary Biomarkers of Oxidative Stress in Children with Chronic Kidney Disease

“…To separate plasma and erythrocytes, the samples were centrifuged (1500× g ; 4 °C, 10 min). Erythrocytes were washed three times in cold 0.9% NaCl ( v : v ) and haemolysed by the addition of cold 50 mM phosphate buffer (pH 7.4) 1:9 ( v : v ) [ 21 ]. In order to prevent sample oxidation, 0.5 M butylated hydroxytoluene (Sigma-Aldrich, Nümbrecht, Germany; 10 μL/mL blood) was added [ 21 ].…”