Exposure to the neonicotinoid imidacloprid disrupts sex allocation cue use during superparasitism in the parasitoid wasp <i><scp>N</scp>asonia vitripennis</i>

Cook, Nicola; Green, Jade; Shuker, David M.; Whitehorn, Penelope R.

doi:10.1111/een.12344

Cited by 22 publications

(12 citation statements)

References 39 publications

(58 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a neurotoxin and agonist of nAChRs, IMD had high binding affinity for nAChRs, thereby disrupting neurotransmission (Ffrench-Constant et al, 2016). IMD was thought to impede information receiving and processing in N. vitripennis , which led to the disruption of sexual communication and foraging behavior (Cook et al, 2016; Tappert et al, 2017). Furthermore, in Solenopsis invicta , when treated with 0.25 μg/ml IMD, there was a significant reduction in food consumption, digging and foraging behavior, while the neurotoxins flubendiamide and indoxacarb increased the walking time of Copidosoma truncatellum (Wang L. et al, 2015; Ramos et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Effect of Sublethal Doses of Imidacloprid on the Biological Performance of Aphid Endoparasitoid Aphidius gifuensis (Hymenoptera: Aphidiidae) and Influence on Its Related Gene Expression

et al. 2018

View full text Add to dashboard Cite

The integrated pest management (IPM) strategy was developed and used in combination with pesticides and beneficial biological control agents. To further develop IPM efficiency, it is important to evaluate the side effects of pesticides on biological control agents. Aphidius gifuensis is one of the most important aphid natural enemies and has been successfully used to control Myzys persicae and other aphid species. Imidacloprid (IMD) is a popular pesticide used worldwide and is highly toxic to non-target arthropods. Here, we investigated the short-term sublethal toxicity of IMD in Aphidius gifuensis and its impact on the biological performance and gene expression of this parasitoid. We found that sublethal IMD doses had a significant negative effect on the life history traits of female A. gifuensis, including shortening the lifespan and lowering parasitic capacity. Moreover, exposure to sublethal IMD also adversely affected the response of A. gifuensis to aphid-infested plant volatiles. Based on the transcriptome analysis, we found that the exposure to sublethal IMD doses significantly affected expression of genes involved in the central nervous system, energy metabolism, olfactory, and detoxification system of A. gifuensis. RT-qPCR also revealed that short term expose to sublethal IMD doses significantly induced the gene expression of genes related to the central nervous system (nAChRa7, nAChRa9, TbH, OAR1, NFR, TYR, and DAR1), olfactory system (OR28 and IR8a1), and detoxification system (CYP49p3, CYP6a2, and POD), while it suppressed the expression of genes involved in the central nervous system (nAChRa4 and nAChRb1), olfactory system (Orco1, IR8a2, and GR1), and detoxification system (GST2). Furthermore, exposure to sublethal doses of IMD also significantly increased the activities of CarEs and POD, whereas we observed no influence on the activities of CAT, GST, and SOD. Our results indicate that sublethal IMD doses might adversely affect the biological performance of A. gifuensis by altering gene expression related to the function of olfactory, nervous, energy metabolism, and detoxification systems. Thus, how the use of pesticides directly affect insect population should be considered when used in conjunction with natural pest parasitoids in IPM strategies.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of Sublethal Doses of Imidacloprid on the Biological Performance of Aphid Endoparasitoid Aphidius gifuensis (Hymenoptera: Aphidiidae) and Influence on Its Related Gene Expression

et al. 2018

View full text Add to dashboard Cite

show abstract

“…We also note that sub-lethal exposure to neurotoxic neonicotinoid pesticides does also influence sex allocation, albeit in the opposite direction to that seen with 5-aza-dC; i.e. more female-biased sex ratios are seen than expected: Whitehorn et al 2015, Cook et al 2016.…”

Section: Quantifying the Efficacy Of 5-aza-dcmentioning

confidence: 66%

Validating the Demethylating Effects of 5-aza-2′-deoxycytidine in Insects Requires a Whole-Genome Approach

Cook

Parker

Tauber

et al. 2019

The American Naturalist

Self Cite

View full text Add to dashboard Cite

We previously demonstrated that treatment with the demethylating agent 5-aza-2′-deoxycytidine (5-aza-dC) alters the offspring sex ratios produced by females of the parasitoid wasp Nasonia vitripennis . Females allocate offspring sex ratio in line with Local Mate Competition theory, producing more or less female-biased sex ratios as the number of other females laying eggs on a patch varies, thereby reducing competition amongst their sons for mates. Interestingly, treatment with 5-aza-dC did not ablate the facultative sex allocation response. Instead, sex ratios became less female-biased, a shift in the direction of the optimum sex ratio for paternally-inherited alleles according to genomic conflict theory. This was the first (albeit indirect) experimental evidence for genomic conflict over sex allocation. Ellers et al. (2019) have since assayed the effects of 5-aza-dC on DNA methylation in ten Nasonia genes, finding no evidence of demethylation in these 10 genes, from which they conclude that 5-aza-dC has no demethylating capability in Nasonia vitripennis. Quantifying the efficacy of 5-aza-dC in terms of demethylation is indeed crucial to in-depth interpretation of studies using 5-aza-dC to link phenotypes to epigenetic regulation. Here, we outline the mode of action of 5-aza-dC and demonstrate that determining the efficacy of 5-aza-dC in insect systems requires a whole-genome approach.

show abstract

“…Therefore, the effects observed here as well as the decreased fecundity and survival found in other species after exposure to neonicotinoids 2 50 , likely compromise the key function of parasitoid wasps as natural enemies in terrestrial ecosystems. More subtle fitness-relevant effects caused by neonicotinoids such as a hampered ability to adjust the optimal offspring sex ratio 45 51 might additionally decrease the reproductive success of parasitoid wasps in the field.…”

Section: Discussionmentioning

confidence: 99%

Sublethal doses of imidacloprid disrupt sexual communication and host finding in a parasitoid wasp

Tappert

Pokorny

Hofferberth

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Neonicotinoids are widely used insecticides, but their use is subject of debate because of their detrimental effects on pollinators. Little is known about the effect of neonicotinoids on other beneficial insects such as parasitoid wasps, which serve as natural enemies and are crucial for ecosystem functioning. Here we show that sublethal doses of the neonicotinoid imidacloprid impair sexual communication and host finding in the parasitoid wasp Nasonia vitripennis. Depending on the dose, treated females were less responsive to the male sex pheromone or unable to use it as a cue at all. Courtship behaviour of treated couples was also impeded resulting in a reduction of mating rates by up to 80%. Moreover, treated females were no longer able to locate hosts by using olfactory cues. Olfaction is crucial for the reproductive success of parasitoid wasps. Hence, sublethal doses of neonicotinoids might compromise the function of parasitoid wasps as natural enemies with potentially dire consequences for ecosystem services.

show abstract

Exposure to the neonicotinoid imidacloprid disrupts sex allocation cue use during superparasitism in the parasitoid wasp Nasonia vitripennis

Cited by 22 publications

References 39 publications

Effect of Sublethal Doses of Imidacloprid on the Biological Performance of Aphid Endoparasitoid Aphidius gifuensis (Hymenoptera: Aphidiidae) and Influence on Its Related Gene Expression

Effect of Sublethal Doses of Imidacloprid on the Biological Performance of Aphid Endoparasitoid Aphidius gifuensis (Hymenoptera: Aphidiidae) and Influence on Its Related Gene Expression

Validating the Demethylating Effects of 5-aza-2′-deoxycytidine in Insects Requires a Whole-Genome Approach

Sublethal doses of imidacloprid disrupt sexual communication and host finding in a parasitoid wasp

Contact Info

Product

Resources

About