Exposure to the BPA-Substitute Bisphenol S Causes Unique Alterations of Germline Function

Chen, Yi‐Chang; Shu, Le; Qiu, Zhiqun; Lee, Dong Yeon; Settle, Sara J.; Hee, Shane S. Que; Telesca, Donatello; Yang, Xia; Allard, Patrick

doi:10.1371/journal.pgen.1006223

Cited by 91 publications

(71 citation statements)

References 57 publications

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“…Yeast vaults packaged MnP also showed improved biocatalytic activity as compared with free MnPs. BPA, which is widely used in plastic and epoxy resin manufacturing, is one of the major endocrine disruptors found in the environment (Chen et al, ), and was used as a model compound to test the efficiency of rMnP‐yMVP. As the least stable MnP, rMnP only removed 30% BPA in 24 hr (Figure b).…”

Section: Resultsmentioning

confidence: 99%

Synthesis and assembly of human vault particles in yeast

Wang

Kickhoefer

Rome

et al. 2018

Biotech & Bioengineering

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Vault particles are the largest naturally occurring ribonucleoprotein complexes found in the cytoplasm. In all 78 copies of major vault protein (MVP) assemble on polyribosome templates, forming recombinant vault particles, which are of great interest as encapsulation carriers for therapeutics delivery and enzyme stabilization. Baculovirus-insect cell expression is the only system that has been developed for recombinant vault synthesis, but it has low scalability and slow production rate. In this study, we demonstrated the first use of yeast cells for the production of vault particles with full integrity and functionality solely by expressing the complementary DNA (cDNA) encoding MVP. Vaults synthesized in Pichia pastoris yeast cells are morphologically indistinguishable from recombinant vault particles produced in insect cells, and are able to package and stabilize enzymes resulting in improved longevity and catalytic efficiency. Thus, our results imply that the yeast system is an economical alternative to insect cells for the production of recombinant vaults. The consistency of vault morphology between yeast and insect cell systems also underlines that polyribosome templating may be conserved among eukaryotes, which promises the synthesis and assembly of recombinant human vault particles in other eukaryotic organisms.

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Section: Resultsmentioning

confidence: 99%

Synthesis and assembly of human vault particles in yeast

Wang

Kickhoefer

Rome

et al. 2018

Biotech & Bioengineering

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“…C. elegans is an ideal system for such studies given that meiosis in this system is well-characterized, this model organism has a rapid life cycle, and its cellular processes and pathways share a high degree of conservation with those found in mammals. Furthermore, C. elegans is a model that provides unique advantages for studies of reprotoxicity, including: (1) nuclei in the adult gonad are organized in a spatial-temporal gradient facilitating the study of meiosis and the clear visualization of alterations in chromosome morphogenesis at different meiotic stages resulting from the action of germline disruptors and (2) it is a highly predictive model of mammalian toxicities [29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Environmentally-relevant exposure to diethylhexyl phthalate (DEHP) alters regulation of double-strand break formation and crossover designation leading to germline dysfunction in Caenorhabditis elegans

et al. 2020

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Exposure to diethylhexyl phthalate (DEHP), the most abundant plasticizer used in the production of polyvinyl-containing plastics, has been associated to adverse reproductive health outcomes in both males and females. While the effects of DEHP on reproductive health have been widely investigated, the molecular mechanisms by which exposure to environmentally-relevant levels of DEHP and its metabolites impact the female germline in the context of a multicellular organism have remained elusive. Using the Caenorhabditis elegans germline as a model for studying reprotoxicity, we show that exposure to environmentallyrelevant levels of DEHP and its metabolites results in increased meiotic double-strand breaks (DSBs), altered DSB repair progression, activation of p53/CEP-1-dependent germ cell apoptosis, defects in chromosome remodeling at late prophase I, aberrant chromosome morphology in diakinesis oocytes, increased chromosome non-disjunction and defects during early embryogenesis. Exposure to DEHP results in a subset of nuclei held in a DSB permissive state in mid to late pachytene that exhibit defects in crossover (CO) designation/ formation. In addition, these nuclei show reduced Polo-like kinase-1/2 (PLK-1/2)-dependent phosphorylation of SYP-4, a synaptonemal complex (SC) protein. Moreover, DEHP exposure leads to germline-specific change in the expression of prmt-5, which encodes for an arginine methyltransferase, and both increased SC length and altered CO designation levels on the X chromosome. Taken together, our data suggest a model by which impairment of a PLK-1/2-dependent negative feedback loop set in place to shut down meiotic DSBs, together with alterations in chromosome structure, contribute to the formation of an excess number of DSBs and altered CO designation levels, leading to genomic instability.

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“…Regarding BPA analogues, only few studies have examined the effects of these BPs on female and the male reproductive systems. BPS elicits repro-ductive and germline toxicity in Caenorhabditis elegans [72]. This reproductive influence was also seen in some mice experiments.…”

Section: Effect On Male and Female Reproductive System Developmentmentioning

confidence: 62%

The dark side of the breastfeeding: In the light of endocrine disruptors

Chemek

Nevoral

2019

Medical Journal of Cell Biology

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Breastfeeding plays an essential role in the healthy development of a newborn, but human milk is obviously compromised by pollutants from our environment. The main contaminants of human milk with endocrine-disrupting compound (EDCs) have raised concern for public and environmental health. Bisphenol A (BPA), which can leach from plastics, are among the most well-studied. Since EDs are known to cross the mammary gland barrier and BPA may accumulate in the neonate, “BPA-free” products have been introduced to the market. However, recent studies have shown that alternative bisphenols (e.g. BPS, BPF) can be detected in breast milk, have ED activities and may have developmental effects similar to BPA.

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Exposure to the BPA-Substitute Bisphenol S Causes Unique Alterations of Germline Function

Cited by 91 publications

References 57 publications

Synthesis and assembly of human vault particles in yeast

Synthesis and assembly of human vault particles in yeast

Environmentally-relevant exposure to diethylhexyl phthalate (DEHP) alters regulation of double-strand break formation and crossover designation leading to germline dysfunction in Caenorhabditis elegans

The dark side of the breastfeeding: In the light of endocrine disruptors

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