Exposure to Sub-Acute Concentrations of Glyphosate Induce Clinico-Hematological, Serum Biochemical and Genotoxic Damage in Adult Cockerels

Abstract

Current experimental study was aimed to find out the clinico-hematological, serum biochemical and DNA damage impacts of commonly used herbicide (glyphosate) on non-target adult cockerels. Therefore, a total of 25 cockerels were randomly placed in wire cages in five different groups each containing five birds. After seven days of acclimatization, glyphosate-based herbicide was administered to cockerels of groups (B-E) @ 50, 75, 100 and 125 mg/kg BW respectively except group A (control) for 45 days. Blood and serum was collected at 15, 30 and 45 day of the trial from each cockerel. The treated birds at higher doses indicated different clinical signs such as ruffled feather, dullness, tremors, anemic wattle and comb, depression and reduced frequency of crowing. Feed consumption and body mass was significantly (P≤0.05) lowered in cockerels exposed to higher doses of herbicide. The hematological parameters including red blood cell counts, hematocrit and hemoglobin was significantly lower in treated cockerels. Results on different biochemical parameters showed significantly lower quantity of total proteins and albumin while significantly higher concentrations of liver function tests (alanine aminotransferase, aspartate aminotransferase, alkaline phosphate), kidney function tests (urea and creatinine), cardiac biomarkers (CK-Mb, triglycerides, cholesterol) and oxidative stress parameter (malondialdehyde) of treated cockerels in dose and time dependent manner. Results indicate that the frequency of cells with tail DNA was significantly (P≤0.001) in exposed cockerels. The findings of the study suggested that long term exposure of glyphosate induces adverse clinicohematological, serum biochemical and genotoxic effects on birds. A, 2019. Exposure to sub-acute concentrations of glyphosate induce clinicohematological, serum biochemical and genotoxic damage in adult cockerels. Pak Vet J, 39(2): 181-186. http://dx.

New Insights Into Hematological, Serum Biochemical and Histopathological Toxicity of Bisphenol a on Bighead Carp (Aristichthys Nobils) Under Long-Term Exposure

“…In the present study, Ash content increased after exposure of sh to high concentrations of bisphenol A. Similar results available previously, (Rao et al, 2010;Hussain et al, 2019). Limited information is available in the literature about an effect of industrial e uents on the proximate composition and amino acid pro le of freshwater shes and their use as a biomarker of toxicant contamination (Hussain et al, 2019).…”

“…Similar results available previously, (Rao et al, 2010;Hussain et al, 2019). Limited information is available in the literature about an effect of industrial e uents on the proximate composition and amino acid pro le of freshwater shes and their use as a biomarker of toxicant contamination (Hussain et al, 2019). The ndings of this research work have indicated that industrial contaminant (bisphenol A) probably had adversely affected the proximate composition of sh meat in A.nobilis.…”

“…Over the past few decades in addition to the pathogenic risk of microbes, the indiscriminate application of synthetic chemicals in agriculture, aquatic life, industries, veterinary practice, protection of environment and to improve public health has become serious threat (Arslan et al, 2017;Richardson et al, 2017;Leem et al, 2017;Hussain et al, 2019). Accidental exposure to industrial chemicals and various environmental polutants in aquatic and terrestrial ecosytems not only cuases deaths but also reduces the life expectancy of several target and non-target organisms (Glassmeyer et al, 2016;Richardson et al, 2017;Ghaffart et al, 2019;Ghaffar et al, 2020).…”

“…While in previous reports, similar results like RBCs reduction decreased in Hb, MCHC, and increase in WBCs and neutrophils were studied in common carp(Ghaffar et al, 2018), African cat sh(Nashwa, 2014;Sisodiya et al, 2018) Labeo rohita(Krishnapriya et al, 2017) and Clarias gariepinus(Pathania et al, 2019) exposed to sublethal concentrations of bisphenol A. Hematological abnormalities may be due to erythrocyte destruction in blood-forming cells, increase the production of free radicles, and poor supply of oxygen through gills. Moreover, many reports of hematological parameters are also available on other vertebrates like the Albino mouse(Moselhy, 2015), Rats(Karnam et al, 2015) Yellow n seabream(Yaghoobi., 2017), and adult cockerels(Hussain et al, 2019) exposed to toxicants. In the present research work, tissue damage observed in bighead carp caused by a higher concentration of BPA.…”

Metabolomics Approach Explore Diagnostic Biomarkers and Metabolic Changes in Heat-Stressed Dairy Cows

“…Instead, we found the upregulation of 3-Methylhistidine(1-MH)in the plasma of heat-stressed dairy cows. 1-MH is the degradation product of anserine (β-alany1-1-methy1-histidine) that is a dipeptide found in the muscles of many animals, including cows [ 33 ]. 1-MH is used as a biomarker for meat intake in humans because anserine is not found in human muscle [ 34 ].…”