Exposure to sediments from polluted rivers has limited phenotypic effects on larvae and adults of Chironomus riparius

Arambourou, Hélène; Beisel, Jean‐Nicolas; Branchu, Philippe; Debat, Vincent

doi:10.1016/j.scitotenv.2014.03.010

Cited by 26 publications

(22 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this regard, some studies based on ecotoxicological laboratory experiments queried the relationship between chironomid deformities and toxicity assessment in freshwater ecosystems [24,49,[72][73][74][75][76]. However, the high variability of those experimental results can be influenced by different variables, such as: (i) the endpoint used (mentum, mandibles, pectin epipharyngis); (ii) the deformity types considered; (iii) the larval instars used in the experiment; (iv) the type of cultures used which often cause a high incidence of deformities in the control test; (v) the exposure time of the larvae to the contaminants; and (vi) the type of contaminant mixture used in the test.…”

Section: Discussionmentioning

confidence: 99%

Use of Larval Morphological Deformities in Chironomus plumosus (Chironomidae: Diptera) as an Indicator of Freshwater Environmental Contamination (Lake Trasimeno, Italy)

Goretti

Pallottini

Pagliarini

et al. 2019

Water

View full text Add to dashboard Cite

The mentum deformity incidence in Chironomus plumosus larvae to assess the environmental contamination level in Lake Trasimeno, Central Italy, was investigated. The survey lasted from May 2018 to August 2019. Fifty-one samplings were carried out: 34 in the littoral zone and 17 in the central zone. The deformity assessment was based on 737 and 2767 larval specimens of C. plumosus collected from the littoral and central zones, respectively. Comparison of the larval morphometric variables between normal and deformed specimens highlighted that the deformities did not cause alterations of the larval growth. The deformity incidence amounted to 7.22% in the whole Trasimeno’s ecosystem, reaching 8.28% in the littoral zone and 6.94% in the central zone. Among the different seasonal cohorts, the spring cohort had overall the highest deformity value (11.41%). The deformity type assessment protocol highlighted that the most common deformity type was “round/filed teeth” (64%). The results of this 2018–2019 survey revealed a low deformity incidence, within the background range of relatively low-impacted freshwaters. Comparison with previous investigations (2000–2010) of the same habitat showed a clear decrease of the deformity incidence. This study further contributes to the evaluation of the mentum deformity in chironomids that represent an indicator endpoint of the anthropogenic contamination level in freshwaters.

show abstract

Section: Discussionmentioning

confidence: 99%

Use of Larval Morphological Deformities in Chironomus plumosus (Chironomidae: Diptera) as an Indicator of Freshwater Environmental Contamination (Lake Trasimeno, Italy)

Goretti

Pallottini

Pagliarini

et al. 2019

Water

View full text Add to dashboard Cite

show abstract

“…Further non-significant results were reported from mites [128] and wings of Trichogramma egg parasitoids [129]-but both studies reported results only from relatively small subsamples (≤30 specimens per sample) and tiny organisms, raising questions about statistical power and possible artifacts from mounting very small specimens. Further negative results come from two datasets of wings from Chironomus midges [116], whereas other studies found mixed results for wings and larval mouthparts [103,130,131]. Some studies, in a variety of species, have found mixed results, with some structures or subgroups yielding significant results, but others not [132][133][134][135][136][137][138].…”

Section: Directional Asymmetrymentioning

confidence: 99%

“…Several studies found greater fluctuating asymmetry in disturbed than in undisturbed habitats for skull shape of voles [250], in areas of worse rather than better climatic suitability for skull shape of Akodon rodents [370], in urban than in rural habitats for head shape of lizards [106], in polluted than unpolluted habitats for crab carapace shape [253] and for mandible shape of shrews [371]. Studies of the larval mentum and adult wings in chironomid midges showed that pollution in the rearing environment had no or only limited effects on fluctuating asymmetry [130,131] or that there was more fluctuating asymmetry in offspring reared in unpolluted sediment in the laboratory than in the parental generation collected from polluted sediment in the field [103]. Temperature stress had no significant effect on fluctuating asymmetry of body shape in bulb mites [372], but fluctuating asymmetry of shape has been shown to be influenced by temperature in aphid [164] and Drosophila wings [62,121].…”

Section: Developmental Instability Of Shape In Relation To Stress Andmentioning

confidence: 99%

Analyzing Fluctuating Asymmetry with Geometric Morphometrics: Concepts, Methods, and Applications

2015

View full text Add to dashboard Cite

Approximately two decades after the first pioneering analyses, the study of shape asymmetry with the methods of geometric morphometrics has matured and is a burgeoning field. New technology for data collection and new methods and software for analysis are widely available and have led to numerous applications in plants and animals, including humans. This review summarizes the concepts and morphometric methods for studying asymmetry of shape and size. After a summary of mathematical and biological concepts of symmetry and asymmetry, a section follows that explains the methods of geometric morphometrics and how they can be used to analyze asymmetry of biological structures. Geometric morphometric analyses not only tell how much asymmetry there is, but also provide information about the patterns of covariation in the structure under study. Such patterns of covariation in fluctuating asymmetry can provide valuable insight about the developmental basis of morphological integration, and have become important tools for evolutionary developmental biology. The genetic basis of fluctuating asymmetry has been studied from empirical and theoretical viewpoints, but serious challenges remain in this area. There are many promising areas for further research that are only little explored at present.

show abstract

“…However, diatom teratologies may affect several structural features (other than overall shape), such as striation pattern and raphe structure, resulting in multiple types of teratologies commonly observed (Arini et al 2013;Falasco et al 2009b;Pandey et al 2014). Geometric morphometry approaches accounting for other types of teratologies are used to characterize deformities in higher organisms, for example, deformities in insect wing structures (Arambourou et al 2014). Including this type of evaluation by incorporating additional landmarks in future investigations would complement our findings and could further improve the assessment of diatom teratologies.…”

Section: Advantages and Limitations Of Morphometric Geometry To Quantmentioning

confidence: 67%

“…This image-based approach relies on the changes in the location of landmarks positioned along the valve outline and/or on specific features (intersection between internal structures). It is a widely-used method for larger organisms such as chironomids (Arambourou et al 2014), drosophila (Debat et al 2003), and fish (Avigliano et al 2017). It has also been used to discriminate between morphologically closely-related diatoms (Fránková et al 2009;Pappas et al 2014;Potapova and Hamilton 2007;Woodard et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Assessing the severity of diatom deformities using geometric morphometry

et al. 2018

View full text Add to dashboard Cite

Deformities in diatoms are increasingly used as an indicator of toxic stress in freshwaters. However, the percentage of deformities alone often fails at highlighting the magnitude of toxic exposure. It has been suggested that the severity of the deformation (degree of departure from normal form) could be a valuable aspect to consider in addition to the percentage of deformities in an assemblage. An approach combining the assessment of deformities coupled with information on their severity could improve the sensitivity of this biomarker.With the aim of quantifying the deviation from the normal form, we tested the applicability of geometric morphometry to evaluate the degree of deformities in different diatom species. We used photomicrographs of normal and deformed specimens from laboratory cultures of Gomphonema gracile, Nitzschia palea, and of Achnanthidium minutissimum from field samples collected along a gradient of toxic contamination. The geometric morphometry approach is based on several landmarks positioned on the outline of the diatom valves, followed by a Procrustes superimposition analysis to determine shape variations.Statistical analyses were conducted based on the geometrical coordinates of the landmarks. This technique allowed to discriminate between normal and deformed individuals. The geometric morphometry approach revealed a gradient in the intensity of the deformities observed on Gomphonema gracile and Achnanthidium minutissimum, in-line with a priori, visually-determined (subjective) classifications. A relationship between the degree of deformity in Achnanthidium minutissimum and a gradient of zinc contamination was found. In contrast, the approach failed to obtain good fit for Nitzschia palea individuals because deformities in this species were more variable in terms of their location on the valves.Geometric morphometry provided encouraging results to objectively quantify the intensity of diatom deformities affecting valve outline, and could easily be implemented in further automatic diatom identification developments.

show abstract

Exposure to sediments from polluted rivers has limited phenotypic effects on larvae and adults of Chironomus riparius

Cited by 26 publications

References 55 publications

Use of Larval Morphological Deformities in Chironomus plumosus (Chironomidae: Diptera) as an Indicator of Freshwater Environmental Contamination (Lake Trasimeno, Italy)

Use of Larval Morphological Deformities in Chironomus plumosus (Chironomidae: Diptera) as an Indicator of Freshwater Environmental Contamination (Lake Trasimeno, Italy)

Analyzing Fluctuating Asymmetry with Geometric Morphometrics: Concepts, Methods, and Applications

Assessing the severity of diatom deformities using geometric morphometry

Contact Info

Product

Resources

About