Exposure to positively- and negatively-charged plasma cluster ions impairs IgE-binding capacity of indoor cat and fungal allergens

Nishikawa, Kazuo; Fujimura, Takashi; Ota, Yusuke; Abe, Takuya; ElRamlawy, Kareem Gamal; Nakano, Miyako; Takado, Tomoaki; Uenishi, A.; Kawazoe, Hidechika; Sekoguchi, Yoshinori; Tanaka, Akihiko; Ono, Kazuhisa; Kawamoto, Seiji

doi:10.1186/s40413-016-0118-z

Cited by 5 publications

(9 citation statements)

References 20 publications

(29 reference statements)

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“…We previously demonstrated that allergens from Japanese cedar pollen and fungus were degraded by PCI treatment in an experimental setting [9,10]. In the present study we confirmed that the PCI treatment also degraded HDM allergens in a manner similar to that reflected by the previous studies' results, by conducting an SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) analysis (data not shown).…”

Section: Discussionsupporting

confidence: 81%

“…We previously demonstrated that PCI treatment effectively inactivated atomized Japanese cedar pollen and fungal allergens [9,10]. Those results prompted us to examine the effect of PCIs on HDM allergens, one of the most important airborne environmental allergens.…”

Section: Discussionmentioning

confidence: 99%

“…The airborne dry HDM particles were treated with 3,000 counts/cm 3 of PCIs for an additional 15 min, and the particles were then collected on a membrane filter by pumping the air out of the chamber for 45 min. The collected HDM particles were suspended in 2 mL of phosphate-buffered saline to evaluate their IgE-binding capacity as a similar method reported in our previous studies [9,10]. …”

Section: Methodsmentioning

confidence: 99%

“…The cylindrical apparatus was filled with PCIs generated from a PCI-generating device (a second-generation Plasma Cluster™ device, Sharp Corp., Osaka, Japan) for 15 min, followed by 8 mL of atomized 200 ng/mL HDM extract solution. The PCI-treated HDM solution was then collected in a petri dish at the bottom of the apparatus [9,10]. A 1-m 3 acrylic box equipped with a PCI-generating device (a second-generation Plasma Cluster™ device, Sharp Corp.) and a stirring fan were used for the evaluation of the effects of PCIs under a simulated indoor environmental condition (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…In our previous studies, we employed a novel air-cleaning technology that emits both positively and negatively charged plasma cluster ions (PCIs) generated by plasma discharge, and we observed that the PCIs significantly reduced the IgE-binding capacity of atomized Japanese cedar pollen allergens and fungal allergens in an experimental cylindrical apparatus [9,10]. …”

Section: Introductionmentioning

confidence: 99%

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Plasma Cluster Ions Reduce the IgE-Binding Capacity of House Dust Mite Allergens under a Simulated Indoor Environmental Condition

Okano

Fujimura²,

Fukuoka³

et al. 2017

Int Arch Allergy Immunol

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Background: A high level of house dust mite (HDM) allergens in a living environment is a risk factor for both sensitization to these allergens and asthmatic attacks. We previously showed that plasma cluster ions (PCIs) impaired the IgE-binding capacity of atomized crude allergens prepared from Japanese cedar pollen and fungus under experimental conditions. Objective: We evaluated the capacity of PCIs to impair the IgE-binding capacity of airborne HDM allergens under a simulated indoor environmental condition. Methods: For the determination of the effects of PCIs on HDM allergens under an experimental condition, HDM extract was atomized as aqueous mist into a cylindrical experimental apparatus filled with PCIs. For the evaluation of the effects of PCIs under a simulated natural indoor environmental condition, dried HDM allergens were floated as airborne particles in an acryl cubic apparatus in the presence of PCIs. The IgE-binding capacities of the PCI- and sham-treated HDM allergens were analyzed by an ELISA. Results: The IgE-binding capacity of the HDM allergens was significantly impaired after PCI treatment compared to that after sham treatment under both experimental and simulated environmental conditions. The ELISA results demonstrated that the IgE-binding capacities of HDM allergens after PCI treatment showed 68 and 74% reductions compared to those after sham treatment under the experimental and simulated environmental conditions, respectively. Conclusions: PCIs have the capacity to impair the IgE-binding capacity of airborne HDM allergens in a simulated environmental condition.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Plasma Cluster Ions Reduce the IgE-Binding Capacity of House Dust Mite Allergens under a Simulated Indoor Environmental Condition

Okano

Fujimura²,

Fukuoka³

et al. 2017

Int Arch Allergy Immunol

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Analysis on the risks of severe adverse events in rabies post-exposure prophylaxis and appropriate decision-making procedure

Huang

Zhu

et al. 2018

Human Vaccines & Immunotherapeutics

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Severe adverse events (AEs) following post-exposure rabies vaccination had been occasionally described in previous studies. Once AEs occurred, immediate medical treatment and appropriate change of vaccine and vaccination schedule were of significance. It was also important and challengeable to determine the relationship among adverse reactions, vaccines residues and laboratory tests for patients, to choose a proper vaccine in resumed vaccination, to avoid the reoccurrence of AEs and to ensure adequate immune response. Here, we present steps about how to cope with AEs by giving an example with a two-year-old girl who was identified as category II exposure to rabies, suffered from anaphylaxis after first dose administration with human diploid rabies vaccine (HDCV) so vaccination was temporarily suspended. Dexamethasone was prescribed to her in anti-allergy therapy. Allergy tests indicated that the patient was not sensitive to allergens and heterologous proteins. Vaccine test report showed that residual kanamycin existed in that batch of vaccines. This reminded us to provide her antibiotic skin sensitivity test which found she was allergic to kanamycin. Thus, we could conclude it was the cause of AEs. Then, 0.5 mL lyophilized Purified Vero Cell Rabies Vaccine (PVRV) without any residues was enrolled in the resumed vaccination. To ensure successful immunization, immunogenicity test was also provided which showed adequate immune response (RVNA ≥ 0.5 IU/mL) starting from day14. Besides, no further AEs occurred afterward. This study emphasized the importance of in-depth survey, analysis and implied the necessity to scientifically and properly choose the optimal vaccine for patients and appropriately provide treatments if AEs occurred.

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World Allergy Organization Journal: the Editors Look Back at 2016

Jensen‐Jarolim

Fiocchi

2017

World Allergy Organization Journal

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Exposure to positively- and negatively-charged plasma cluster ions impairs IgE-binding capacity of indoor cat and fungal allergens

Cited by 5 publications

References 20 publications

Plasma Cluster Ions Reduce the IgE-Binding Capacity of House Dust Mite Allergens under a Simulated Indoor Environmental Condition

Plasma Cluster Ions Reduce the IgE-Binding Capacity of House Dust Mite Allergens under a Simulated Indoor Environmental Condition

Analysis on the risks of severe adverse events in rabies post-exposure prophylaxis and appropriate decision-making procedure

World Allergy Organization Journal: the Editors Look Back at 2016

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