Exposure to perfluorinated compounds: in vitro study on thyroid cells

Coperchini, Francesca; Pignatti, Patrizia; Lacerenza, Serena; Negri, Sara; Sideri, Riccardo; Testoni, Claudia; Martinis, Luca de; Cottica, Danilo; Magri, Flavia; Imbriani, Marcello; Rotondi, Mario; Chiovato, Luca

doi:10.1007/s11356-014-3480-9

Cited by 48 publications

(18 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, under the different conditions of our study, the MTT conversion percentage was increased by PFOA at the doses investigated. Contrary to studies showing that PFOS and PFOA did not increase the proliferation of the cells at concentrations below inhibitory concentrations (Shan et al, 2013;Coperchini et al, 2014), our results revealed that both PFASs were able to increase MTT conversion at concentrations below toxic level. Hormesis is a reproducible and generalizable phenomenon that explains many dose-response relationships (Calabrese et al, 1999).…”

Section: Discussioncontrasting

confidence: 99%

In vitro evaluation of the cytotoxicity and modulation of mechanisms associated with inflammation induced by perfluorooctanesulfonate and perfluorooctanoic acid in human colon myofibroblasts CCD-18Co

Giménez‐Bastida

Surma

Zieliński

2015

Toxicology in Vitro

View full text Add to dashboard Cite

Section: Discussioncontrasting

confidence: 99%

In vitro evaluation of the cytotoxicity and modulation of mechanisms associated with inflammation induced by perfluorooctanesulfonate and perfluorooctanoic acid in human colon myofibroblasts CCD-18Co

Giménez‐Bastida

Surma

Zieliński

2015

Toxicology in Vitro

View full text Add to dashboard Cite

“…Laboratory studies on thyroid cell cultures showed that in vitro exposure of thyroid cells to several PFAS can have various thyroid-disrupting effects. An accumulation of PFOS and PFOA was documented in thyroid cells, and a cytotoxic effect was observed after exposure to extremely high concentrations of these compounds ( 37 ). In addition, PFOS and PFOA reduce thyroperoxidase (TPO) activity in tumor thyroid cells lines ( 38 ) and PFOS was suggested to act as a thyroid hormone receptor (THR) agonist in GH3 cells as found by using THRα and THRβ-mediated luciferase reporter assay ( 39 ).…”

Section: Introductionmentioning

confidence: 99%

Thyroid Disrupting Effects of Old and New Generation PFAS

et al. 2021

Self Cite

View full text Add to dashboard Cite

Per- and polyfluoroalkyl substances (PFAS) represent a group of synthetic compounds widely used in industry plants due to their low grade of degradation, surfactant properties, thermic and flame resistance. These characteristics are useful for the industrial production, however they are also potentially dangerous for human health and for the environment. PFAS are persistent pollutants accumulating in waters and soil and recoverable in foods due to their release by food packaging. Humans are daily exposed to PFAS because these compounds are ubiquitous and, when assimilated, they are difficult to be eliminated, persisting for years both in humans and animals. Due to their persistence and potential danger to health, some old generation PFAS have been replaced by newly synthesized PFAS with the aim to use alternative compounds presumably safer for humans and the environment. Yet, the environmental pollution with PFAS remains a matter of concern worldwide and led to large-scale epidemiological studies both on plants’ workers and on exposed people in the general population. In this context, strong concern emerged concerning the potential adverse effects of PFAS on the thyroid gland. Thyroid hormones play a critical role in the regulation of metabolism, and thyroid function is related to cardiovascular disease, fertility, and fetal neurodevelopment. In vitro, ex vivo data, and epidemiological studies suggested that PFASs may disrupt the thyroid hormone system in humans, with possible negative repercussions on the outcome of pregnancy and fetal-child development. However, data on the thyroid disrupting effect of PFAS remain controversial, as well as their impact on human health in different ages of life. Aim of the present paper is to review recent data on the effects of old and new generation PFAS on thyroid homeostasis. To this purpose we collected information from in vitro studies, animal models, and in vivo data on exposed workers, general population, and pregnant women.

show abstract

“…Previous in vitro studies investigated the toxic effects of several PFAS in different types of cultured cells including thyroid cells [3,[17][18][19][20][21]. However, no information is still available regarding the in vitro effects of C6O4 on thyroid cells.…”

Section: Introductionmentioning

confidence: 99%

The new generation PFAS C6O4 does not produce adverse effects on thyroid cells in vitro

Coperchini

Croce

Pignatti

et al. 2020

J Endocrinol Invest

View full text Add to dashboard Cite

Purpose Per- and poly-fluoroalkyl-substances (PFASs) are synthetic compounds that raised concern due to their potential adverse effects on human health. Long-chain PFAS were banned by government rules in many states, and thus, new emerging PFAS were recently introduced as substitutes. Among these, Perfluoro{acetic acid, 2-[(5-methoxy-1,3-dioxolan-4-yl)oxy]}, ammonium salt (C6O4) was recently introduced to produce a range of food contact articles and literature data about this compound are scanty. The aim of this study was to evaluate the in vitro effects of exposure to C6O4, compared with PFOA and PFOS on thyroid cells. Methods FRTL5 rat-thyroid cell lines and normal human thyroid cells (NHT) were incubated with increasing concentrations of C6O4 for 24, 48, 72, and 144 h to assess cell viability by WST-1. Cell viability was confirmed by AnnexinV/PI staining. Long-chain PFAS (PFOA and PFOS) were used at same concentrations as positive controls. The proliferation of cells exposed to C6O4, PFOA, and PFOS was measured by staining with crystal violet and evaluation of optical density after incubation with SDS. Changes in ROS production by FRTL5 and NHT after exposure to C6O4 at short (10, 20, and 30 min) and long-time points (24 h) were evaluated by cytofluorimetry. Results C6O4 exposure did not modify FRTL5 and NHT cell viability at any concentration and/or time points with no induction of necrosis/apoptosis. At difference, PFOS exposure reduced cell viability of FRTL5 while and NHT, while PFOA only in FRTL5. FRTL5 and NHT cell proliferation was reduced by incubation with by PFOA and PFOS, but not with C6O4. ROS production by NHT and FRTL5 cells was not modified after C6O4 exposure, at any time/concentration tested. Conclusions The present in vitro study constitutes the first evaluation of the potential adverse effects of the new emerging PFAS C6O4 in cultured rat and human thyroid cells, suggesting its safety for thyroid cells in vitro.

show abstract

Exposure to perfluorinated compounds: in vitro study on thyroid cells

Cited by 48 publications

References 35 publications

In vitro evaluation of the cytotoxicity and modulation of mechanisms associated with inflammation induced by perfluorooctanesulfonate and perfluorooctanoic acid in human colon myofibroblasts CCD-18Co

In vitro evaluation of the cytotoxicity and modulation of mechanisms associated with inflammation induced by perfluorooctanesulfonate and perfluorooctanoic acid in human colon myofibroblasts CCD-18Co

Thyroid Disrupting Effects of Old and New Generation PFAS

The new generation PFAS C6O4 does not produce adverse effects on thyroid cells in vitro

Contact Info

Product

Resources

About