Exposure to mite and cat allergens on a range of clothing items at home and the transfer of cat allergen in the workplace

Lucca, Sandra D. De; O'Meara, T.J.; Tovey, Euan R.

doi:10.1067/mai.2000.110804

Cited by 95 publications

(85 citation statements)

References 22 publications

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“…These high allergen levels may be due to allergen transfer from the workplace through the clothes and hair of farmers. Human hair and clothing have been reported as a main carrier for mouse and cat allergens [37,38,39]. The data presented here support this route of exposure based on the high bovine allergen burden measured in changing rooms.…”

Section: Discussionsupporting

confidence: 73%

Development of a Sandwich ELISA to Measure Exposure to Occupational Cow Hair Allergens

Zahradnik

Sander

Bruckmaier

et al. 2011

Int Arch Allergy Immunol

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Background: Cow hair and dander are important inducers of occupational allergies in cattle-exposed farmers. To estimate allergen exposure in farming environments, a sensitive enzyme immunoassay was developed to measure cow hair allergens. Methods: A sandwich ELISA was developed using polyclonal rabbitantibodies against a mixture of hair extracts from different cattle breeds. To assess the specificity of the assay, extracts from other mammalian epithelia, mites, molds and grains were tested. To validate the new assay, cow hair allergens were measured in passive airborne dust samples from the stables and homes of farmers. Dust was collected with electrostatic dust fall collectors (EDCs). Results: The sandwich ELISA was found to be very sensitive (detection limit: 0.1 ng/ml) and highly reproducible, demonstrating intra- and interassay coefficients of variation of 4 and 10%, respectively. The assay showed no reactivity with mites, molds and grains, but some cross-reactivity with other mammalian epithelia, with the strongest reaction with goat. Using EDCs for dust sampling, high concentrations of bovine allergens were measured in cow stables (4,760–559,400 µg/m²). In addition, bovine allergens were detected in all areas of cattle farmer dwellings. A large variation was found between individual samples (0.3–900 µg/m²) and significantly higher values were discovered in changing rooms. Conclusion: The ELISA developed for the detection of cow hair proteins is a useful tool for allergen quantification in occupational and home environments. Based on its low detection limit, this test is sensitive enough to detect allergens in passive airborne dust.

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Section: Discussionsupporting

confidence: 73%

Development of a Sandwich ELISA to Measure Exposure to Occupational Cow Hair Allergens

Zahradnik

Sander

Bruckmaier

et al. 2011

Int Arch Allergy Immunol

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“…Usually, very little dust is collected from clothing by vacuum sampling (<0.1 g), and in most cases, small amounts of mites or allergen are recovered [80••,81]. Two studies report extrapolated mean Der p 1 allergen densities on various types of clothing [82,83]. The significance of extrapolated quantities is difficult to evaluate because they represent amounts that do not occur on clothing.…”

Section: Mites Found On Clothesmentioning

confidence: 98%

Dust mite allergens: Ecology and distribution

Arlian

Morgan

Neal

2002

Curr Allergy Asthma Rep

111

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The most important allergy-causing mites found in homes worldwide are the house dust mites Dermatophagoides farinae, D. pteronyssinus, Euroglyphus maynei, and the storage mite Blomia tropicalis. Most homes contain multiple species. The most prevalent mite species and allergen in homes differ geographically, between homes within a geographical region, and among areas within a home. Therefore, it is important to know which mite species are present in a geographical area when performing diagnostic testing and prescribing immunotherapy. The key factor that influences mite survival and prevalence is relative humidity. Mites are present in homes in humid geographical areas and are rare or absent in drier climates unless humidity is artificially raised. Generally speaking, dust mite allergen levels are low in public buildings and transportation compared to levels in homes.

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“…Median levels of allergens were lower in living rooms (4 g/m 2 ), bedrooms (12 g/m 2 ), and kitchens (19 g/m 2 ) and higher in home office (63 g/m 2 ) and changing rooms (105 g/m 2 ), suggesting that bovine allergens are spread from the workplace via the clothes and hair of farmers. Similarly, transport of mouse, cat, wheat allergen and fungal a-amylase from work to home through clothes, shoes and human hair has previously been reported (Krop et al, 2007;De Lucca et al, 2000;Karlsson and Renström, 2005;Vissers et al, 2001). Also for microorganisms a transfer between stable and home is suggested.…”

Section: Tablementioning

confidence: 89%

Exposure levels, determinants and IgE mediated sensitization to bovine allergens among Danish farmers and non-farmers

Schlünssen

Basinas

Zahradnik

et al. 2015

International Journal of Hygiene and Environmental Health

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Exposure to mite and cat allergens on a range of clothing items at home and the transfer of cat allergen in the workplace

Cited by 95 publications

References 22 publications

Development of a Sandwich ELISA to Measure Exposure to Occupational Cow Hair Allergens

Development of a Sandwich ELISA to Measure Exposure to Occupational Cow Hair Allergens

Dust mite allergens: Ecology and distribution

Exposure levels, determinants and IgE mediated sensitization to bovine allergens among Danish farmers and non-farmers

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