Exposure to metal oxide nanoparticles in physiological fluid induced synergistic biological effects in a keratinocyte model

Cathe, Deidre S.; Whitaker, Jasmine N.; Breitner, Emily K.; Comfort, Kristen K.

doi:10.1016/j.toxlet.2017.01.003

Cited by 13 publications

(9 citation statements)

References 38 publications

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“…Therefore, the next goal was to characterize the HepG2 stress response following exposure to the experimental, 70 nm PtNPs ( Figure 3 ). First, intracellular ROS levels were monitored, as its production is a documented precursor for apoptosis and a known cellular response following NP exposure [ 26 , 27 ]. As shown in Figure 3(a) , the PtNPs induced ROS production in a dose-dependent fashion, with a substantial response associated with the 25 μ g/mL condition.…”

Section: Resultsmentioning

confidence: 99%

Toxicological Implications of Platinum Nanoparticle Exposure: Stimulation of Intracellular Stress, Inflammatory Response, and Akt Signaling In Vitro

Labrador-Rached

Browning

Braydich‐Stolle

et al. 2018

Journal of Toxicology

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Due to their distinctive physicochemical properties, platinum nanoparticles (PtNPs) have emerged as a material of interest for a number of biomedical therapeutics. However, in some instances NP exposure has been correlated to health and safety concerns, including cytotoxicity, activation of cellular stress, and modification to normal cell functionality. As PtNPs have induced differential cellular responses in vitro, the goal of this study was to further characterize the behavior and toxicological potential of PtNPs within a HepG2 liver model. This study identified that a high PtNP dosage induced HepG2 cytotoxicity. However, lower, subtoxic PtNP concentrations were able to elicit multiple stress responses, secretion of proinflammatory cytokines, and modulation of insulin-like growth factor-1 dependent signal transduction. Taken together, this work suggests that PtNPs would not be overtly toxic for acute exposures, but sustained cellular interactions might produce long term health consequences.

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Section: Resultsmentioning

confidence: 99%

Toxicological Implications of Platinum Nanoparticle Exposure: Stimulation of Intracellular Stress, Inflammatory Response, and Akt Signaling In Vitro

Labrador-Rached

Browning

Braydich‐Stolle

et al. 2018

Journal of Toxicology

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“…Discrepancies between these models are due in part to the fact that NP physicochemical properties and dosimetry are dependent upon environmental factors. For example, when NPs are dispersed in physiological fluids, such as interstitial and lysosomal, the rate of ionic dissolution and extent of agglomeration are significantly altered versus cell culture media [ 53 , 54 ]. Additionally, NP exposure within a three-dimensional in vitro model diminished the extent of cytotoxic responses versus two-dimensional models, owing to the need for NPs to translocate through the cell systems prior to internalization [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Characterizing the Role of Biologically Relevant Fluid Dynamics on Silver Nanoparticle Dependent Oxidative Stress in Adherent and Suspension In Vitro Models

et al. 2021

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Silver nanoparticles (AgNPs) are being employed in numerous consumer goods and applications; however, they are renowned for inducing negative cellular consequences including toxicity, oxidative stress, and an inflammatory response. Nanotoxicological outcomes are dependent on numerous factors, including physicochemical, biological, and environmental influences. Currently, NP safety evaluations are carried out in both cell-based in vitro and animal in vivo models, with poor correlation between these mechanisms. These discrepancies highlight the need for enhanced exposure environments, which retain the advantages of in vitro models but incorporate critical in vivo influences, such as fluid dynamics. This study characterized the effects of dynamic flow on AgNP behavior, cellular interactions, and oxidative stress within both adherent alveolar (A549) and suspension monocyte (U937) models. This study determined that the presence of physiologically relevant flow resulted in substantial modifications to AgNP cellular interactions and subsequent oxidative stress, as assessed via reactive oxygen species (ROS), glutathione levels, p53, NFκB, and secretion of pro-inflammatory cytokines. Within the adherent model, dynamic flow reduced AgNP deposition and oxidative stress markers by roughly 20%. However, due to increased frequency of contact, the suspension U937 cells were associated with higher NP interactions and intracellular stress under fluid flow exposure conditions. For example, the increased AgNP association resulted in a 50% increase in intracellular ROS and p53 levels. This work highlights the potential of modified in vitro systems to improve analysis of AgNP dosimetry and safety evaluations, including oxidative stress assessments.

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“…AIF, presented by Stopford et al (2003), has been used to predict the behaviour of inhaled exogenous material, for example CuO and TiO 2 (Cathe et al 2017). However, this is not exclusive to the respiratory system and primarily mimics the fluids surrounding cells within skin tissue.…”

Section: Artificial Interstitial Fluid (Aif)mentioning

confidence: 99%

Simulated biological fluids – a systematic review of their biological relevance and use in relation to inhalation toxicology of particles and fibres

Innes

Yiu

McLean

et al. 2021

Critical Reviews in Toxicology

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Exposure to metal oxide nanoparticles in physiological fluid induced synergistic biological effects in a keratinocyte model

Cited by 13 publications

References 38 publications

Toxicological Implications of Platinum Nanoparticle Exposure: Stimulation of Intracellular Stress, Inflammatory Response, and Akt Signaling In Vitro

Toxicological Implications of Platinum Nanoparticle Exposure: Stimulation of Intracellular Stress, Inflammatory Response, and Akt Signaling In Vitro

Characterizing the Role of Biologically Relevant Fluid Dynamics on Silver Nanoparticle Dependent Oxidative Stress in Adherent and Suspension In Vitro Models

Simulated biological fluids – a systematic review of their biological relevance and use in relation to inhalation toxicology of particles and fibres

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