Exposure to mercury causes formation of male-specific structural deficits by inducing oxidative damage in nematodes

Liu, Peidang; He, Ke-Wen; Li, Yinxia; Wu, Qiuli; Yang, Peng; Wang, Dayong

doi:10.1016/j.ecoenv.2011.12.007

Cited by 55 publications

(16 citation statements)

References 51 publications

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“…In C. elegans , toxicants usually cause damage on animals by inducing oxidative stress [18], [29], [53]. We further examined the intestinal ROS production in clentuberol or ractopamine exposed nematodes.…”

Section: Resultsmentioning

confidence: 99%

Adverse Effects from Clenbuterol and Ractopamine on Nematode Caenorhabditis elegans and the Underlying Mechanism

Zhuang

Zhao

et al. 2014

PLoS ONE

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In the present study, we used Caenorhabditis elegans assay system to investigate in vivo toxicity from clentuberol and ractopamine and the possible underlying mechanism. Both acute and prolonged exposures to clentuberol or ractopamine decreased brood size and locomotion behavior, and induced intestinal autofluorescence and reactive oxygen species (ROS) production. Although acute exposure to the examined concentrations of clentuberol or ractopamine did not induce lethality, prolonged exposure to 10 µg/L of clentuberol and ractopamine reduced lifespan. At relatively high concentrations, ractopamine exhibited more severe toxicity than clentuberol on nematodes. Overexpression of sod-2 gene encoding a Mn-SOD to prevent induction of oxidative stress effectively inhibited toxicity from clentuberol or ractopamine. Besides oxidative stress, we found that clentuberol might reduce lifespan through influencing insulin/IGF signaling pathway; however, ractopamine might reduce lifespan through affecting both insulin/IGF signaling pathway and TOR signaling pathway. Ractopamine more severely decreased expression levels of daf-16, sgk-1, skn-1, and aak-2 genes than clentuberol, and increased expression levels of daf-2 and age-1 genes at the examined concentration. Therefore, the C. elegans assay system may be useful for assessing the possible toxicity from weight loss agents, and clentuberol and ractopamine may induce toxicity through different molecular mechanisms.

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Section: Resultsmentioning

confidence: 99%

Adverse Effects from Clenbuterol and Ractopamine on Nematode Caenorhabditis elegans and the Underlying Mechanism

Zhuang

Zhao

et al. 2014

PLoS ONE

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“…Nematodes pre-treated with 1 mg/mL of WGH for 48-hr were transferred to the 35°C condition for 16–hr or 2 mmol/L of paraquat for 6–hr [30], [41], and then the lifespan and the intestinal ROS production were measured as described above. Paraquat was used to induce the oxidative stress in nematodes [30].…”

Section: Methodsmentioning

confidence: 99%

Beneficial Effects of Wheat Gluten Hydrolysate to Extend Lifespan and Induce Stress Resistance in Nematode Caenorhabditis elegans

Zhang

et al. 2013

PLoS ONE

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Previous studies have showed that wheat gluten hydrolysate (WGH) has the anti-oxidative property. In the present study, we examined the possible safety property of WGH and the beneficial effects of WGH to extend lifespan and induce stress resistance using nematode Caenorhabditis elegans as the in vivo assay system. We found that WGH at concentrations of 0.1–1 mg/mL did not cause lethality, influence development, alter locomotion behavior and brood size, and induce significant intestinal autofluorescence and reactive oxygen species (ROS) production in young adults. Treatment with 0.1–1 mg/mL of WGH significantly extended lifespans of nematodes under the normal conditions. Moreover, WGH treatment significantly inhibited the induction of intestinal autofluorescence and suppressed the decrease in locomotion behavior during the aging process of nematodes. Furthermore, pre-treatment with 1 mg/mL of WGH significantly suppressed the adverse effects caused by heat-stress or oxidative stress on nematodes as indicated by the alterations of both lifespan and intestinal ROS production. Therefore, WGH treatment is relatively safe and has beneficial effects on nematodes under both the normal conditions and the stress conditions.

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“…33 In our comparison of the upper limits of potential elemental exposure (teabacco cigarettes versus traditional tobacco cigarettes), we identified 190 μg (0.19 mg) of lead per 20 teabacco cigarettes smoked. While there is currently no universally accepted allowance for safe lead ingestion, 34,35 a previous tolerable weekly intake of 25 μg per kg body weight was deemed unacceptably high. 31,36,37 As a result, any individuals smoking a 'pack-a-day' of teabacco each week are at risk of some negative health effects due to potential exposure nearing the withdrawn weekly intake.…”

Section: Identification Of Compounds By Gc-msmentioning

confidence: 99%

Constituents of “teabacco”: A forensic analysis of cigarettes made from diverted nicotine replacement therapy lozenges in smoke‐free prisons

Mitchell

Puljević

Coomber

et al. 2018

Drug Testing and Analysis

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Following the implementation of prison smoke-free policies, there have been reports of prisoners creating substitute cigarettes made from nicotine replacement therapy patches or lozenges infused with tea leaves ("teabacco"). No studies have analyzed the chemical constituents of teabacco made from nicotine lozenges, so as to document any potential related health hazards. Teabacco samples were made by a participant who reported creating teabacco while incarcerated in a smoke-free prison in Queensland, Australia, and the process was video-recorded for replication in a laboratory. A simple linear smoking system captured the teabacco smoke for analysis. Inductively coupled plasma optical emission spectroscopy (ICP-OES) was used to analyze elemental composition and gas chromatography coupled with a mass spectrometer (GC-MS) analyzed the captured smoke using the National Institute of Standards and Technology mass spectral library. Analyses determined that quantities of copper, aluminum, and lead concentrations, and levels of inhaled total particulate matter, were above recommended guidelines for safe ingestion. Analysis of teabacco smoke using GC-MS identified potentially toxic compounds catechol and nicotine. However, our findings show that smoking this form of teabacco is less harmful than smoking teabacco made from nicotine patches, or smoking traditional tobacco cigarettes. Considering the limited potential health harm of smoking teabacco made from lozenges, and that nicotine lozenges represent the only form of smoking cessation support for individuals entering smoke-free prisons, we caution against the removal of nicotine lozenges from Queensland's prisons, at least until further research directly establishes health harms associated with this form of teabacco.

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Exposure to mercury causes formation of male-specific structural deficits by inducing oxidative damage in nematodes

Cited by 55 publications

References 51 publications

Adverse Effects from Clenbuterol and Ractopamine on Nematode Caenorhabditis elegans and the Underlying Mechanism

Adverse Effects from Clenbuterol and Ractopamine on Nematode Caenorhabditis elegans and the Underlying Mechanism

Beneficial Effects of Wheat Gluten Hydrolysate to Extend Lifespan and Induce Stress Resistance in Nematode Caenorhabditis elegans

Constituents of “teabacco”: A forensic analysis of cigarettes made from diverted nicotine replacement therapy lozenges in smoke‐free prisons

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