Exposure to magnetic field (5 mT at 60 Hz) does not affect cell growth and c-myc gene expression.

Miyakoshi, Junji; Ohtsu, Shuji; Shibata, Toru; Takebe, Hiraku

doi:10.1269/jrr.37.185

Cited by 39 publications

(16 citation statements)

References 27 publications

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“…The exposure apparatus for 5 mT ELFMF is described elsewhere (19). Environmental 60 Hz ELFMF during the sham exposure was Ͻ0.5 T. Static magnetic fields other than geomagnetism were undetectable (Ͻ0.1 T) for all experiments and residual geomagnetism was Ͻ1 T. The atmosphere in the incubator for both units was saturated with humidified 95% air plus 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

Increase in X-Ray-Induced Mutations by Exposure to Magnetic Field (60 Hz, 5 mT) in NF-κB-Inhibited Cells

Ding

Yaguchi

Yoshida

et al. 2000

Biochemical and Biophysical Research Communications

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Section: Methodsmentioning

confidence: 99%

Increase in X-Ray-Induced Mutations by Exposure to Magnetic Field (60 Hz, 5 mT) in NF-κB-Inhibited Cells

Ding

Yaguchi

Yoshida

et al. 2000

Biochemical and Biophysical Research Communications

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“…ELFMF exposure, a sinusoidal magnetic field at a frequency of 60 Hz, 5 mT, was performed using a previously described magnetic field exposure apparatus [Miyakoshi et al, 1996;Ding et al, 2000]. The distribution of the magnetic density was measured using a Gauss meter (Model 3251, Yokogawa Electrical Co., Ltd., Tokyo).…”

Section: Elfmf Exposure Unitmentioning

confidence: 99%

An extremely low frequency magnetic field attenuates insulin secretion from the insulinoma cell line, RIN‐m

Sakurai

Satake

Sumi

et al. 2004

Bioelectromagnetics

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In this study, we investigated the effects of exposure to an extremely low frequency magnetic field (ELFMF) on hormone secretion from an islet derived insulinoma cell line, RIN-m. We stimulated RIN-m cells to secrete insulin under exposure to an ELFMF, using our established system for the exposure of cultured cells to an ELFMF at 5 mT and 60 Hz, or under sham exposure conditions for 1 h and observed the effects. In the presence of a depolarizing concentration of potassium (45 mM KCl), exposure to ELFMF significantly attenuated insulin release from RIN-m cells, compared to sham exposed cells. Treatment with nifedipine reduced the difference in insulin secretion between cells exposed to an ELFMF and sham exposed cells. The expression of mRNA encoding synaptosomal associated protein of 25 kDa (SNAP-25) and synaptotagmin 1, which play a role in exocytosis in hormone secretion and influx of calcium ions, decreased with exposure to an ELFMF in the presence of 45 mM KCl. These results suggest that exposure to ELFMF attenuates insulin secretion from RIN-m cells by affecting calcium influx through calcium channels.

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“…For example, low-intensity MF was reported to induce cmyc transcription [Phillips et al, 1992], increase HSP70 synthesis [Pipkin et al, 1999], and enhance ornithine decarboxylase (ODC) activity in L929 cells [Litovitz et al, 1991]. However, there are also reports showing MF does not affect these endpoints [Miyakoshi et al, 1996;Morehouse and Owen, 2000a,b]. Therefore, further studies are needed to solve the apparent inconsistency of these results.…”

Section: Introductionmentioning

confidence: 99%

Noise magnetic fields abolish the gap junction intercellular communication suppression induced by 50 hz magnetic fields

Zeng¹,

Ke²,

Gao³

et al. 2006

Bioelectromagnetics

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Previously, we have reported that exposure to 50 Hz coherent sinusoidal magnetic fields (MF) for 24 h inhibits gap junction intercellular communication (GJIC) in mammalian cells at an intensity of 0.4 mT and enhances the inhibition effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) at 0.2 mT. In the present study, we further explored the effects of incoherent noise MF on MF-induced GJIC inhibition. GJIC was determined by fluorescence recovery after photobleaching (FRAP) with a laser-scanning confocal microscope. The rate of fluorescence recovery (R) at 10 min after photobleaching was adopted as the functional index of GJIC. The R-value of NIH3T3 cells exposed to 50 Hz sinusoidal MF at 0.4 mT for 24 h was 30.85 +/- 14.70%, while the cells in sham exposure group had an R-value of 46.36 +/- 20.68%, demonstrating that the GJIC of NIH3T3 cells was significantly inhibited by MF exposure (P < .05). However, there were no significant differences in the R-values of the sham exposure, MF-plus-noise MF exposure (R: 49.58 +/- 19.38%), and noise MF exposure groups (R: 46.74 +/- 21.14%) (P > .05), indicating that the superposition of a noise MF alleviated the suppression of GJIC induced by the 50 Hz MF. In addition, although MF at an intensity of 0.2 mT synergistically enhanced TPA-induced GJIC inhibition (R: 24.90 +/- 13.50% vs. 35.82 +/- 17.18%, P < .05), further imposition of a noise MF abolished the synergistic effect of coherent MF (R: 32.51 +/- 18.37%). Overall, the present data clearly showed that although noise MF itself had no effect on GJIC of NIH3T3 cells, its superposition onto a coherent sinusoidal MF at the same intensity abolished MF-induced GJIC suppression. This is the first report showing that noise MF neutralizes 50 Hz MF-induced biological effect by using a signaling component as the test endpoint.

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Exposure to magnetic field (5 mT at 60 Hz) does not affect cell growth and c-myc gene expression.

Cited by 39 publications

References 27 publications

Increase in X-Ray-Induced Mutations by Exposure to Magnetic Field (60 Hz, 5 mT) in NF-κB-Inhibited Cells

Increase in X-Ray-Induced Mutations by Exposure to Magnetic Field (60 Hz, 5 mT) in NF-κB-Inhibited Cells

An extremely low frequency magnetic field attenuates insulin secretion from the insulinoma cell line, RIN‐m

Noise magnetic fields abolish the gap junction intercellular communication suppression induced by 50 hz magnetic fields

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