Exposure to iron ore attenuates the reproductive potential of adult male Wistar rats

Nwangwa, Eze Kingsley; Ekhoye, E. I.; Ologun, Seyi Rachael; Anuta, Alan

doi:10.5455/jeos.20150422071350

Cited by 2 publications

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Section: Resultsmentioning

confidence: 99%

“…An increase in the level of the liver enzymes shows impairment in hepatic function [22], [23]. The ALT enzyme localized in the liver parenchyma cells is more sensitive in indicating any damage to the hepatocytes when compared to AST, which is mostly found in the mitochondria [22]. The test result showed that there was no significant change in the levels of AST and ALT though the value of ALT in group 3 was slightly increased but still not significant, therefore partly opposing Nwangwaet al and Theresa and Jonas's findings [22], [23].…”

Section: Resultsmentioning

confidence: 99%

“…The sera were later centrifuged at 3,000 revolutions per minute (RPM) for 10 minutes to let any minute clot sediment. These sera were refrigerated at 5 °C and the sera Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), and total bilirubin were estimated using the Reitman and Frankel method [22]. Prolactin was assayed using prolactin Enzyme-Linked Immunosorbent Assay (ELISA) method in the Enzymology Department of Federal Teaching Hospital Abakaliki, Nigeria.…”

Section: Experimental Designmentioning

confidence: 99%

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Effects of Oral Consumption of Nicotiana Tabacum During Pregnancy on the Liver and Prolactin Levels of Adult Female Wistar Rats

Udeh

Ikwuka²,

Epete³

et al. 2023

EJVETMED

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Introduction: Tobacco (Nicotiana tabacum) is a globally consumed plant that contains harmful alkaloids such as nicotine. Aim: To study the effects of tobacco consumption during pregnancy on the liver and prolactin levels of adult female Wistar rats. Materials and Methods: The research was done using 21 female Wistar rats which were randomly and evenly separated into groups I, II and III. Pregnant rats in groups II (GII) and III(GIII) received 0.32 g/kg and 0.64 g/kg body weight of tobacco respectively with the use of an oral gavage while pregnant rats in the control group (GI) did not receive the stock solution. On the 23rd day, blood samples and the livers were collected for examination. Blood samples were collected from the mother rats forthe estimation of Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), and total bilirubin levels. The liverswere stained with Hematoxylin and Eosin stains to examine the histological architecture. Results: Liver enzyme assays on the mother rats showed that AST for GII (54.67±3.06 iu/L) and GIII(55.00±7.02 iu/L) were not significantly different (p>0.05) from GI(56.00±0.00 iu/L) using Tukey post hoc test. ALT for GII (41.67±3.51 iu/L) and GIII (47.00±2.58 iu/L) were also not significantly different (p>0.05) from GI (45.00±1.41 iu/L). The total bilirubin value for GII (0.87±0.29 mg/dL) was not significantly different (p>0.05) from GI (0.85±0.07 mg/dL), but total bilirubin for GIII (1.88±0.22 mg/dL) was significantly different(p<0.05) to that of GI. There was a significant decrease(p<0.05) in prolactin levels of mother rats in GII (1.75±0.35 ng/ml) and GIII (1.50±0.71 ng/ml) when compared to GI (11.15±0.35 ng/ml). Histological findings showed that the liver of mother rats in GII and GIII were damaged. Conclusion: The consumption of tobacco (Nicotiana tabacum) by female Wistar rats during pregnancy inhibits prolactin secretion and severely alters theliver histological architecture.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

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Effects of Oral Consumption of Nicotiana Tabacum During Pregnancy on the Liver and Prolactin Levels of Adult Female Wistar Rats

Udeh

Ikwuka²,

Epete³

et al. 2023

EJVETMED

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Toxicological outcome of phthalate exposure on male fertility: Ameliorative impacts of the co-administration of N-acetylcysteine and zinc sulfate in rats

Emojevwe

Nwangwa

Naiho

et al. 2022

Middle East Fertil Soc J

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Background Reports have shown that humans are consistently exposed to environmental toxicants such as phthalate (PHT) during their daily activities. This results in reproductive dysfunction and infertility-related issues as already noted in human and experimental animals. We therefore designed this study to investigate fertility outcome in phthalate-exposed male rats treated with N-acetylcysteine (NAC) and zinc sulfate (ZnSO4) with the view of providing a therapeutic alternative to reproductive toxicity caused by phthalate. The research was done in two phases. In phase 1, thirty-five male Wistar rats were randomly assigned to one of five (n = 7) groups given the following treatments for 21 days: group A was given distilled water as a control, while groups B, C, D, and E were given phthalate (750 mg/kg/day). Animals in groups C to E were also given ZnSO4 (0.5 mg/kg/day), N-acetylcysteine (100 mg/kg/day), and ZnSO4 (0.5 mg/kg/day) + N-acetylcysteine (100 mg/kg/day) in addition to phthalate. In phase 2, animals from groups in phase 1 were mated with females for fecundity testing. Results The result shows alteration in testicular and epididymis weight and testis/epididymis ratio, semen parameters, sperm capacitation and acrosome reaction, sperm DNA, serum Zn and Mg, testicular mitochondria apoptosis mechanisms (TNF-α and BCL-2), and testicular Ca2+-ATPase as well as fecundity outcome in the phthalate-treated group. However, ZnSO4 and NAC successfully ameliorated the deleterious effects of phthalate on semen parameters, sperm capacitation and acrosome reaction, serum electrolyte and mitochondria apoptosis mechanisms, and testicular electrogenic Ca2+-ATPase in phthalate-induced male rats with a better outcome in the combined therapy. Pregnancy outcome and litter sizes were also higher in the combined therapy when also compared with the phthalate-treated groups. Conclusion According to the result, ZnSO4 and NAC increased fertility outcome in phthalate-treated male rats through enhancement of testicular BCL-2, serum electrolyte, testicular Ca2+ATPase pumps, and cytoprotection.

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Exposure to iron ore attenuates the reproductive potential of adult male Wistar rats

Cited by 2 publications

References 0 publications

Effects of Oral Consumption of Nicotiana Tabacum During Pregnancy on the Liver and Prolactin Levels of Adult Female Wistar Rats

Effects of Oral Consumption of Nicotiana Tabacum During Pregnancy on the Liver and Prolactin Levels of Adult Female Wistar Rats

Toxicological outcome of phthalate exposure on male fertility: Ameliorative impacts of the co-administration of N-acetylcysteine and zinc sulfate in rats

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