2014
DOI: 10.3389/fgene.2014.00207
|View full text |Cite
|
Sign up to set email alerts
|

Exposure to hycanthone alters chromatin structure around specific gene functions and specific repeats in Schistosoma mansoni

Abstract: Schistosoma mansoni is a parasitic plathyhelminth responsible for intestinal schistosomiasis (or bilharzia), a disease affecting 67 million people worldwide and causing an important economic burden. The schistosomicides hycanthone, and its later proxy oxamniquine, were widely used for treatments in endemic areas during the twentieth century. Recently, the mechanism of action, as well as the genetic origin of a stably and Mendelian inherited resistance for both drugs was elucidated in two strains. However, seve… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

0
18
0

Year Published

2015
2015
2019
2019

Publication Types

Select...
7
1

Relationship

2
6

Authors

Journals

citations
Cited by 14 publications
(18 citation statements)
references
References 59 publications
0
18
0
Order By: Relevance
“…We had shown before that S. mansoni worms exposed transiently to hycanthone, an anthelmintic drug, possess 57 days later a characteristic chromatin profile different from unexposed worms (Roquis et al . ). At that time, we were not able to tell if the drug directly induced the different profiles, or if it acted as a selection agent, killing most of the parasites in the population, except those with a pre‐existing distinct chromatin structure.…”
Section: Discussionmentioning
confidence: 97%
“…We had shown before that S. mansoni worms exposed transiently to hycanthone, an anthelmintic drug, possess 57 days later a characteristic chromatin profile different from unexposed worms (Roquis et al . ). At that time, we were not able to tell if the drug directly induced the different profiles, or if it acted as a selection agent, killing most of the parasites in the population, except those with a pre‐existing distinct chromatin structure.…”
Section: Discussionmentioning
confidence: 97%
“…As a qPCR normalizer control we used the gene promoter region for the Sm Val19 gene (Smp_123090), which was not expressed either in the HDACi- or the vehicle-treated schistosomula assays, and has no histone acetylation and methylation marks at its promoter region, as seen in the public ChIP-Seq datasets from [37] available at the Schistosoma genome browser (http://schistosoma.usp.br). …”
Section: Methodsmentioning
confidence: 99%
“…To assess further evidence for the transcription start site (TSS) of S . mansoni genes, we downloaded the Chromatin Immunoprecipitation Sequencing (ChIP-Seq) dataset of adult worm Histone H3K4me3 (SRR1107840) [ 9 ], and we used the HOMER pipeline [ 35 ] to map the ChIP-Seq sequences to the genome and to find the genomic coordinates of H3K4me3 enriched peaks. HOMER found enriched peaks by calculating the density of the reads at the peaks that should be at least 4-fold higher than the peaks in the surrounding 10 kb region [ 35 ].…”
Section: Methodsmentioning
confidence: 99%
“…Our gene models (Trinity-assembled contigs) and all the histone mark data [ 9 ] mapped to the genome are accessible through a local installation of the UCSC Genome Browser in a Box (GBiB) [ 36 ] at http://schistosoma.usp.br/ .…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation