Exposure to human chorionic gonadotropin during in vitro maturation does not improve the maturation rate and developmental potential of immature oocytes from patients with polycystic ovary syndrome

Ge, Heng; Huang, Xuefeng; Zhang, Wei; Zhao, Junzhao; Lin, Jin-Jun; Zhou, Wei

doi:10.1016/j.fertnstert.2007.02.021

Cited by 40 publications

(26 citation statements)

References 45 publications

(69 reference statements)

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“…There is a limited number of reports describing the embryological aspects of IVM [4,8,17,18] particularly their developmental competence. This is a critical point in IVM procedures, as there are concerns that this approach leads to relatively high rates of developmental incompetent oocytes, which may affect gestation and subsequent birth rates.…”

Section: Discussionmentioning

confidence: 99%

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Fertilization outcome, embryo development and birth after unstimulated IVM

Bös-Mikich

Ferreira²,

Höher³

et al. 2010

J Assist Reprod Genet

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Background/Purposes A common observation in oocyte in vitro maturation (IVM) cycles is poor embryo quality. However, no study was dedicated to assess zygote and early cleavage embryo quality in IVM cycles. The objective of this study is to analyze fertilization outcome, embryo development and the resulting pregnancy and births in unstimulated IVM cycles. Methods IVM oocytes were collected 36 h post hCG and matured in vitro for 28-30 h. All oocytes were inseminated by ICSI. Resulting zygotes and embryos were assessed on day-1, 2 and 3, when transfers were made. Results The overall oocyte maturation and fertilization rates were 63% and 62%, respectively. Abnormal fertilization rate was 1.7%. Ninety five and 14.6% of the 2Pn zygotes reached the 2-cell and 8 -cell stage at day-2 and day-3, respectively. Embryo quality assessment on day-3 at transfer revealed that only 9% of the embryos were of very good quality. Most embryos showed developmental delay. An average of 3.29 embryos were transferred per patient resulting in implantation and clinical gestation rates of 16% and 32%, respectively. Overall 14 healthy babies were born and there is one ongoing pregnancy. ConclusionResults show a significant rate of abnormal fertilization and poor embryo quality after IVM, which is reflected in a higher than average number of embryos being transferred. However, pregnancy, implantation and birth rates are reasonably high and allow us to consider IVM a valuable approach for the treatment of infertility in PCO or PCOS patients.

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Section: Discussionmentioning

confidence: 99%

“…IVM reported success rates vary considerably among services [1,2,4,8,11,17,18,19,21]. It is acknowledged that the methodology for IVM of oocytes collected from unstimulated ovaries and embryo development is still not ideal [12].…”

Section: Introductionmentioning

confidence: 99%

Fertilization outcome, embryo development and birth after unstimulated IVM

Bös-Mikich

Ferreira²,

Höher³

et al. 2010

J Assist Reprod Genet

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“…Mean attempt number for all index cycles combined was 2.1. Most cycles were undertaken for male-factor infertility, although other diagnoses included oligo-ovulation [1], PCOS [1], tubal factor [2], unexplained [4], and diminished ovarian reserve [5]. An average of 12.5 oocytes was retrieved per cycle, 6.6 of which were subjected to IVM for an average of an additional 23.1 h subsequent to completion of first day ICSI.…”

Section: Patient and Cycle Demographicsmentioning

confidence: 99%

“…The literature regarding in-vitro maturation, however, is clouded by the use of identical terminology to describe disparate practices and techniques; IVM of immature oocytes has been described in stimulated or unstimulated cycles [5][6][7], cumulus retained or cumulus stripped oocytes [8,9], and for varying time periods of maturation, ranging from 4 to 48 h [2,3,10,11]. The diverse situations in which in-vitro maturation has been employed makes comparison between studies difficult, especially when laboratory techniques, culture media, and stimulatory types are not explicitly delineated.…”

Section: Introductionmentioning

confidence: 99%

Extended in vitro maturation of immature oocytes from stimulated cycles: an analysis of fertilization potential, embryo development, and reproductive outcomes

Reichman

Politch

Ginsburg

et al. 2010

J Assist Reprod Genet

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“…Although there is some research on this approach (27,28) , advanced studies on the role of gonadotropins are still needed to better understand the process of oocytes IVM. Serum in the culture media is a source of albumin and steroid precursor as well as growth factors for cells.…”

Section: Culture Mediamentioning

confidence: 99%