Exposure to high fluoride concentration in drinking water will affect spermatogenesis and steroidogenesis in male albino rats

Pushpalatha, T.; Marmamula, Srinivas; Reddy, P. Sreenivasula

doi:10.1007/s10534-005-0336-2

Cited by 65 publications

(35 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Sodium fluoride (NaF) was shown to elicit physiological effects in numerous clinical and laboratory studies, with the toxicity of NaF decreasing fertility in most animal species tested (Freni, 1994;Ortiz-P erez et al, 2003;Lu et al, 2014). An intensive literature search revealed that fluoride negatively affects sperm function, including morphology, motility, capacitation, and the acrosome reaction (Chinoy et al, 1994;Elbetieha et al, 2000;Pushpalatha et al, 2005; Dvora 0 kova 0 -Hortova 0 et al Izquierdo-Vega et al, 2008;Spittle, 2009;Sun et al, 2010) all of which are the key process in fertilization, both in vitro and in vivo Kwon et al, 2014a). Additionally, according to National Research Council annual report (ShraderFrechette, 2007), fluoride is an endocrine disruptor altering normal endocrine functions thus might affect sperm function by binding to its receptors on spermatozoa.…”

Section: Introductionmentioning

confidence: 99%

Effect of sodium fluoride on male mouse fertility

et al. 2015

View full text Add to dashboard Cite

SUMMARYSodium fluoride (NaF), an environmental pollutant, has been tested for its impact on fertility in several species of laboratory animals. A literature demonstrated that NaF adversely affects sperm motility, morphology, capacitation, and the acrosome reaction. However, the molecular mechanisms underlying these alterations have not yet been elucidated. Therefore, present study was designed to evaluate the regulatory pathways involved in the effect of NaF on sperm function and fertilization. In this in vitro study, mouse spermatozoa were incubated with a range of concentrations (2.5, 5, and 10 mM) of NaF for 90 min in media that support in vitro fertilization. Our results showed that NaF was associated with reduced intracellular ATP generation, motility, and motion kinematics. Likewise, short-term exposure of spermatozoa to NaF significantly reduced the intracellular calcium concentration, protein kinase-A activity, and tyrosine phosphorylation of sperm proteins, which were associated with a significant decrease in the rate of capacitation and the acrosome reaction. Finally, NaF significantly reduced the fertilization and blastocyst formation during early embryonic development. On the basis of these results, we propose that NaF reduces sperm motility, capacitation, and the acrosome reaction leading to poor fertilization and suppressed embryonic development.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of sodium fluoride on male mouse fertility

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Esse defeito espermático pode ter ocorrido como um artefato de coleta, em conseqüência de um possível choque térmico (SILVA et al, 1993). Estudos em animais de laboratório demonstraram que o consumo de NaF na água de beber (5, 10 e 150ppm) diminui a qualidade espermática (CHINOY et al, 1995;CHINOY et al, 1997;PUSHPALATHA et al, 2005;WAN et al, 2006), bem como induz alterações histológicas no epitélio seminífero (WAN et al, 2006). Em um experimento in vitro com sêmen ovino de animais de produção, foi observado decréscimo na motilidade espermática após adição de diferentes concentrações de NaF (20, 100, 200μmol L -1 e 0,1mol L -1 ) (ZAKRZEWSKA et al, 2002).…”

Section: Resultsunclassified

Qualidade seminal e histomorfometria dos órgãos reprodutivos de ovinos tratados com fluoreto de sódio

Filappi¹,

Prestes²,

Rocha³

et al. 2008

Cienc. Rural

View full text Add to dashboard Cite

“…The testosterone is an important androgen produced in the testis and is involved in the initiation of spermatogenesis [14]. Many studies have reported the male reproductive defects induced by fluoride, such as the deleterious effects of NaF on testosterone level, spermatogenesis, sperm motility and morphology, and fertilizing ability [1,15,16].…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Boerhaavia Diffusa L. On Disruption of Gonadotropins and Testosterone in Fluoride Intoxicated Male Rats

Aggarwal

Khan

2017

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Objective: The present study explored the influence of fluoride exposure on gonadotropins and reproductive hormones.Methods: Male Wistar albino rats weighing 100-150 g were administered with 100, 200, and 300 ppm/kg body weight of sodium fluoride daily for 20 and 40 days. The control group was given 1 ml deionized water/kgbw/day for the same period. At the end of the experimental period, half of the animals were sacrificed and blood serum was collected and testis sample was taken concurrently. Remaining half rats were administrated with two doses (250 mg and 500 mg/kg body weight) of leaf extract of Boerhaavia diffusa L. for another 20 days. Serum level of follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone, and intratesticular level of testosterone were evaluated.Results: It was found that fluoride intoxication resulted in significant increase in the level of FSH (p<0.01) and LH (p<0.0001) while significant decrease in the serum (p<0.01) and testis (p<0.0001) level of testosterone. A significant (p<0.0001) positive correlation was found between level of serum fluoride and gonadotropins: FSH (20 days, r=0.708 and 40 days, r=0.795) and LH (20 days, r=0.779 and 40 days, r=0.891). However, there was significant (p<0.0001) negative correlation existed between serum fluoride and testosterone levels in serum (20 days, r=−0.780 and 40 days, r=−0.862) as well as testis (20 days, r=−0.915 and 40 days, r=−0.938). Conclusion:It was found that administration of B. diffusa L. alleviated the adverse effects of fluoride on reproductive endocrine functions.

show abstract

Exposure to high fluoride concentration in drinking water will affect spermatogenesis and steroidogenesis in male albino rats

Cited by 65 publications

References 23 publications

Effect of sodium fluoride on male mouse fertility

Effect of sodium fluoride on male mouse fertility

Qualidade seminal e histomorfometria dos órgãos reprodutivos de ovinos tratados com fluoreto de sódio

Efficacy of Boerhaavia Diffusa L. On Disruption of Gonadotropins and Testosterone in Fluoride Intoxicated Male Rats

Contact Info

Product

Resources

About